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| id: ECO:0000267
name: enzyme-linked immunoabsorbent assay evidence
def: "A type of protein detection assay evidence where an antigen is bound to a substrate, and an antibody directly or indirectly linked to an enzyme is utilized to determine the amount of bound antigen which is visualized with a color change in the substrate." [ECO:MCC, ECO:SW, PMID:23949770]
synonym: "ELISA evidence" EXACT 
synonym: "enzyme-linked immunosorbent assay" NARROW 
xref: [ MI:0411 "enzyme linked immunosorbent assay"]
is_a: ECO:0005517 ! protein detection assay evidence
comment: ELISA is sometimes used in the study of transcriptional regulation to identify a produced protein, especially secreted protein.
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