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Rensing, C , Fan, B , Sharma, R , Mitra, B and Rosen, BP (2000) CopA: An Escherichia coli Cu(I)-translocating P-type ATPase. Proc. Natl. Acad. Sci. U.S.A. 97:652-6


The copA gene product, a putative copper-translocating P-type ATPase, has been shown to be involved in copper resistance in Escherichia coli. The copA gene was disrupted by insertion of a kanamycin gene through homologous recombination. The mutant strain was more sensitive to copper salts but not to salts of other metals, suggesting a role in copper homeostasis. The copper-sensitive phenotype could be rescued by complementation by a plasmid carrying copA from E. coli or copB from Enterococcus hirae. Expression of copA was induced by salts of copper or silver but not zinc or cobalt. Everted membrane vesicles from cells expressing copA exhibited ATP-coupled accumulation of copper, presumably as Cu(I). The results indicate that CopA is a Cu(I)-translocating efflux pump that is similar to the copper pumps related to Menkes and Wilson diseases and provides a useful prokaryotic model for these human diseases.


PubMed PMC15385


Adenosine Triphosphatases/genetics; Adenosine Triphosphatases/metabolism; Bacterial Proteins/genetics; Bacterial Proteins/metabolism; Biological Transport; Cadmium/metabolism; Cadmium/pharmacology; Carrier Proteins/genetics; Carrier Proteins/metabolism; Cation Transport Proteins; Cell Membrane/metabolism; Copper/metabolism; Copper/pharmacology; Copper Radioisotopes/diagnostic use; Escherichia coli/drug effects; Escherichia coli/enzymology; Escherichia coli/genetics; Mutagenesis; Plasmids/genetics; Recombinant Fusion Proteins/genetics; Recombinant Fusion Proteins/metabolism; Silver/pharmacology; Zinc/metabolism; Zinc/pharmacology

Main Points of the Paper

Please summarize the main points of the paper.

Materials and Methods Used

Please list the materials and methods used in this paper (strains, plasmids, antibodies, etc).

  • Preparation of Everted Membrane Vesicles (Rensing et al 2000)
  • 64 Cu Transport Assay (Rensing et al 2000)

Phenotype Annotations

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Phenotype of Taxon Information Genotype Information (if known) Condition Information OMP ID OMP Term Name ECO ID ECO Term Name Notes Status

a mutation or genetic difference within a strain

  • Taxon: Escherichia Coli
  • Strain: K-12
  • Substrain: W3110
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: copA
  • Genotype of Experimental Strain : copA(del) In strain LMG194
  • Reference Condition:


decreased resistance to antimicrobial compound


enzyme-linked immunoabsorbent assay evidence

Sensitivity to antimicrobial agent Copper Ion. See growth curve in figure 2.


OMP Term: Has been requested for metal resistance.


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