OMP ST:71091 ! Escherichia coli K-12 BW25113 pgk::SPA-kan

Strain Summary

Strain Name	Escherichia coli K-12 BW25113 pgk::SPA-kan
Synonyms
Taxon Information	Pangenome: Escherichia coli Subspecies and/or strain: K-12 NCBI Taxonomy ID: 83333
Genotype	DE(araD-araB)567 DE(rhaD-rhaB)568 F- Lambda- hsdR514 lacZ4787(del)(::rrnB-3) rph-1 pgk::SPA-kan
Strain Reference	PMID:21185072
Strain availability
Ancestry	parent:OMP ST:800 ! Escherichia coli K-12 BW25113
Annotated phenotypes
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Phenotype Annotation

Annotation ID	Qualifier	OMP ID	OMP term name	Relative phenotype information	Experimental condition	ECO ID	ECO term name	Reference	Annotation Extension	Notes
OMP_AN:37487		OMP:0006044	decreased resistance to beta-lactam	Relative to: OMP_AN:27531 Genotype differences: +lacZ4787(del)( +pgk -lacZ4787(del) Condition differences: no differences	temperature:37C medium:LB agar medium:Ampicillin	ECO:0007005	high throughput direct assay evidence used in manual assertion	PMID:21185072	towards some (CHEBI:28971) Ampicillin	chemical concentration= 1 ug/ml normalized fitness= -4.41
OMP_AN:37488		OMP:0007896	decreased resistance to a surfactant	Relative to: OMP_AN:27581 Genotype differences: +lacZ4787(del)( +pgk -lacZ4787(del) Condition differences: no differences	temperature:37C medium:LB agar medium:Benzalkonium chloride	ECO:0007005	high throughput direct assay evidence used in manual assertion	PMID:21185072	towards some (CHEBI:3020) Benzalkonium chloride	chemical concentration= 10 ug/ml normalized fitness= -3.61
OMP_AN:37489		OMP:0007249	decreased resistance to a chemical	Relative to: OMP_AN:27592 Genotype differences: +lacZ4787(del)( +pgk -lacZ4787(del) Condition differences: no differences	temperature:37C medium:LB agar medium:Calcofluor White M2R (F3543 Fluorescent Brightener 28)	ECO:0007005	high throughput direct assay evidence used in manual assertion	PMID:21185072	towards some (CHEBI:50010) Calcofluor White M2R (F3543 Fluorescent Brightener 28)	chemical concentration= 50 ug/ml normalized fitness= -3.78
OMP_AN:37490		OMP:0007249	decreased resistance to a chemical	Relative to: OMP_AN:27511 Genotype differences: +lacZ4787(del)( +pgk -lacZ4787(del) Condition differences: no differences	temperature:37C medium:LB agar medium:(-)-Epinephrine	ECO:0007005	high throughput direct assay evidence used in manual assertion	PMID:21185072	towards some (CHEBI:28918) (-)-Epinephrine	chemical concentration= 1000 uM normalized fitness= -3.97
OMP_AN:37491		OMP:0007249	decreased resistance to a chemical	Relative to: OMP_AN:27505 Genotype differences: +lacZ4787(del)( +pgk -lacZ4787(del) Condition differences: no differences	temperature:37C medium:LB agar medium:Methotrexate hydrate	ECO:0007005	high throughput direct assay evidence used in manual assertion	PMID:21185072	towards some (CID:165528) Methotrexate hydrate	chemical concentration= 1 ug/ml normalized fitness= -4.7
OMP_AN:37492		OMP:0007879	decreased resistance to an antimicrobial agent	Relative to: OMP_AN:27564 Genotype differences: +lacZ4787(del)( +pgk -lacZ4787(del) Condition differences: no differences	temperature:37C medium:LB agar medium:Phleomycin	ECO:0007005	high throughput direct assay evidence used in manual assertion	PMID:21185072	towards some (CHEBI:75044) Phleomycin	chemical concentration= 0.2 ug/ml normalized fitness= -3.98
OMP_AN:37493		OMP:0007520	increased population growth in high osmolarity	Relative to: OMP_AN:27595 Genotype differences: +lacZ4787(del)( +pgk -lacZ4787(del) Condition differences: no differences	temperature:37C medium:LB agar medium:NaCl	ECO:0007005	high throughput direct assay evidence used in manual assertion	PMID:21185072	towards some (CHEBI:26710) NaCl	chemical concentration= 600 mM normalized fitness= 3.93
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References

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