OMP ST:1026 ! Escherichia coli K-12 JW3595

From OMPwiki

Strain Summary

Strain Name

Escherichia coli K-12 JW3595

Synonyms

JW3595-2

Taxon Information
  • Pangenome: Escherichia coli
  • Subspecies and/or strain: K-12
  • NCBI Taxonomy ID: 83333
Genotype

DE(araD-araB)567 DE(rhaD-rhaB)568 F- Lambda- hsdR514 lacZ4787(del)(::rrnB-3) rph-1 rfaF732(del)::kan

Strain Reference

Coli Genetic Stock Center

Strain availability

Coli Genetic Stock Center

Ancestry

parent:OMP ST:800 ! Escherichia coli K-12 BW25113

Annotated phenotypes



Notes

Phenotype Annotation

Annotation ID Qualifier OMP ID OMP term name Relative phenotype information Experimental condition ECO ID ECO term name Reference Annotation Extension Notes

OMP_AN:937

OMP:0007092

decreased cell motility by swarming

Relative to: OMP_AN:881


Genotype differences:

+DE(araD-araB)567 +DE(rhaD-rhaB)568 +hsdR514 +lacZ4787(del)( +rfaF732(del) -IN(rrnD-rrnE)1


Condition differences:

no differences

  • temperature:33C
  • medium:swarming motility medium
ECO:0005803

motility assay evidence

PMID:17122336[1]

OMP_AN:1141

OMP:0005324

altered cell motility by swimming

Relative to: OMP_AN:882


Genotype differences:

+DE(araD-araB)567 +DE(rhaD-rhaB)568 +hsdR514 +lacZ4787(del)( +rfaF732(del) -IN(rrnD-rrnE)1


Condition differences:

no differences

  • temperature:33C
  • medium:swimming motility medium
ECO:0005803

motility assay evidence

PMID:17122336[1]

OMP_AN:33776

OMP:0006036

decreased resistance to macrolide

Relative to: OMP_AN:27518


Genotype differences:

+lacZ4787(del)( +rfaF732(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Azithromycin
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:2955) Azithromycin

  • chemical concentration= 1 ug/ml
  • normalized fitness= -7.97

OMP_AN:33777

OMP:0007249

decreased resistance to a chemical

Relative to: OMP_AN:27571


Genotype differences:

+lacZ4787(del)( +rfaF732(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:CCCP (Carbonyl cyanide 3-chlorophenylhydrazone)
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:3259) CCCP (Carbonyl cyanide 3-chlorophenylhydrazone)

  • chemical concentration= 2 ug/ml
  • normalized fitness= -3.69

OMP_AN:33778

OMP:0006044

decreased resistance to beta-lactam

Relative to: OMP_AN:27535


Genotype differences:

+lacZ4787(del)( +rfaF732(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Cefsulodin sodium salt hydrate
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:31380) Cefsulodin sodium salt hydrate

  • chemical concentration= 24 ug/ml
  • normalized fitness= -5.29

OMP_AN:33779

OMP:0007250

increased resistance to a chemical

Relative to: OMP_AN:27511


Genotype differences:

+lacZ4787(del)( +rfaF732(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:(-)-Epinephrine
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:28918) (-)-Epinephrine

  • chemical concentration= 250 uM
  • normalized fitness= 3.82

OMP_AN:33780

OMP:0006036

decreased resistance to macrolide

Relative to: OMP_AN:27520


Genotype differences:

+lacZ4787(del)( +rfaF732(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Erythromycin
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:42355) Erythromycin

  • chemical concentration= 10 ug/ml
  • normalized fitness= -3.61

OMP_AN:33781

OMP:0007879

decreased resistance to an antimicrobial agent

Relative to: OMP_AN:27515


Genotype differences:

+lacZ4787(del)( +rfaF732(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Fusidic acid sodium salt
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CID:126963539) Fusidic acid sodium salt

  • chemical concentration= 50 ug/ml
  • normalized fitness= -7.03

OMP_AN:33782

OMP:0006044

decreased resistance to beta-lactam

Relative to: OMP_AN:27537


Genotype differences:

+lacZ4787(del)( +rfaF732(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Mecillinam
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:51208) Mecillinam

  • chemical concentration= 0.03 ug/ml
  • normalized fitness= -3.46

OMP_AN:33783

OMP:0007249

decreased resistance to a chemical

Relative to: OMP_AN:27513


Genotype differences:

+lacZ4787(del)( +rfaF732(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Phenazine methosulfate (PMS)
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:8055) Phenazine methosulfate (PMS)

  • chemical concentration= 0.1 mM
  • normalized fitness= -4.25

OMP_AN:33784

OMP:0007249

decreased resistance to a chemical

Relative to: OMP_AN:27573


Genotype differences:

+lacZ4787(del)( +rfaF732(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Procaine hydrochloride
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:8431) Procaine hydrochloride

  • chemical concentration= 10 mM
  • normalized fitness= -4.15

OMP_AN:33785

OMP:0007878

increased resistance to an antimicrobial agent

Relative to: OMP_AN:27553


Genotype differences:

+lacZ4787(del)( +rfaF732(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Puromycin dihydrochloride
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:8642) Puromycin dihydrochloride

  • chemical concentration= 5 ug/ml
  • normalized fitness= 5.52

OMP_AN:33786

OMP:0006040

decreased resistance to quinolone

Relative to: OMP_AN:27543


Genotype differences:

+lacZ4787(del)( +rfaF732(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Streptonigrin
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:9287) Streptonigrin

  • chemical concentration= 0.5 ug/ml
  • normalized fitness= -4.83

OMP_AN:33787

OMP:0006058

increased resistance to aminoglycoside

Relative to: OMP_AN:27529


Genotype differences:

+lacZ4787(del)( +rfaF732(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Tobramycin
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:28864) Tobramycin

  • chemical concentration= 0.05 ug/ml
  • normalized fitness= 4.04

OMP_AN:33788

OMP:0007879

decreased resistance to an antimicrobial agent

Relative to: OMP_AN:27588


Genotype differences:

+lacZ4787(del)( +rfaF732(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Triclosan/Irgasan
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:164200) Triclosan/Irgasan

  • chemical concentration= 0.05 ug/ml
  • normalized fitness= -15.74

OMP_AN:33789

OMP:0007250

increased resistance to a chemical

Relative to: OMP_AN:27568


Genotype differences:

+lacZ4787(del)( +rfaF732(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:(±)-Verapamil hydrochloride
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:53188) (±)-Verapamil hydrochloride

  • chemical concentration= 0.1 mM
  • normalized fitness= 4.11

OMP_AN:33790

OMP:0006041

increased resistance to quinolone

Relative to: OMP_AN:27539


Genotype differences:

+lacZ4787(del)( +rfaF732(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Ciprofloxacin hydrochloride hydrate
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:59936) Ciprofloxacin hydrochloride hydrate

  • chemical concentration= 0.008 ug/ml
  • normalized fitness= 3.69

OMP_AN:33791

OMP:0006040

decreased resistance to quinolone

Relative to: OMP_AN:27541


Genotype differences:

+lacZ4787(del)( +rfaF732(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Nalidixic acid sodium salt
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CID:3864541) Nalidixic acid sodium salt

  • chemical concentration= 2 ug/ml
  • normalized fitness= -4.7

OMP_AN:33792

OMP:0007010

decreased population growth at alkaline pH

Relative to: OMP_AN:27623


Genotype differences:

+lacZ4787(del)( +rfaF732(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:TAPS
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (**SID:24900259) TAPS

  • chemical concentration= pH 8
  • normalized fitness= -7.32

OMP_AN:33793

OMP:0007008

decreased population growth at acidic pH

Relative to: OMP_AN:27622


Genotype differences:

+lacZ4787(del)( +rfaF732(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:MES-HOMOPIPES
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (**SID:329817955 SID:57651422) MES-HOMOPIPES

  • chemical concentration= pH 4
  • normalized fitness= -3.43

OMP_AN:33794

OMP:0007009

increased population growth at acidic pH

Relative to: OMP_AN:27622


Genotype differences:

+lacZ4787(del)( +rfaF732(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:MES-HOMOPIPES
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (**SID:329817955 SID:57651422) MES-HOMOPIPES

  • chemical concentration= 6
  • normalized fitness= 6.5

OMP_AN:33795

OMP:0007879

decreased resistance to an antimicrobial agent

Relative to: OMP_AN:27591


Genotype differences:

+lacZ4787(del)( +rfaF732(del) -lacZ4787(del)


Condition differences:

no differences

ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:45924 + CHEBI:9331) Trimethoprim (CHEBI:45924) + Sulfamethizole (CHEBI:9331)

  • chemical concentration= 0.1 + 50 ug/ml
  • normalized fitness= -5.04


Additional Information

Links

References

See Help:References for how to manage references in OMPwiki.

  1. 1.0 1.1 Inoue, T et al. (2007) Genome-wide screening of genes required for swarming motility in Escherichia coli K-12. J. Bacteriol. 189 950-7 PubMed OMPwiki page
  2. 2.00 2.01 2.02 2.03 2.04 2.05 2.06 2.07 2.08 2.09 2.10 2.11 2.12 2.13 2.14 2.15 2.16 2.17 2.18 2.19 Nichols, RJ et al. (2011) Phenotypic landscape of a bacterial cell. Cell 144 143-56 PubMed OMPwiki page