Category:ECO:0006081 ! far-Western blotting evidence used in manual assertion

id: ECO:0006081

name: far-Western blotting evidence used in manual assertion
def: "A type of physical interaction evidence that is used in a manual assertion that is based on detection of protein-protein interactions by separation of target proteins by SDS-PAGE which are blotted to a membrane, followed by denaturation and renaturation, probing with purified bait proteins, and detection of the target-bait complexes." [PMID:18079728]
synonym: "IPI: far-Western analysis" BROAD []
is_a: ECO:0000076 ! far-Western blotting evidence
is_a: ECO:0000353 ! physical interaction evidence used in manual assertion
relationship: used_in: ECO:0000218 ! manual assertion
intersection_of: ECO:0000076 ! far-Western blotting evidence
intersection_of: used_in ECO:0000218 ! manual assertion

Last version checked
releases/2019-06-14.

14:06:2019 08:25.

rctauber

Last updated
releases/2019-06-14

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