Category:ECO:0000076 ! far-Western blotting evidence

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id: ECO:0000076

name: far-Western blotting evidence
def: "A type of physical interaction evidence that is based on detection of protein-protein interactions by separation of target proteins by SDS-PAGE which are blotted to a membrane, followed by denaturation and renaturation, probing with purified bait proteins, and detection of the target-bait complexes." [PMID:18079728]
synonym: "IPI: far-Western analysis" RELATED []
xref: [ MI:0047]
is_a: ECO:0000021 ! physical interaction evidence
comment: The interacting protein is referenced in the evidence_with column.


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