PMID:4945191

Citation	Eriksson-Grennberg, KR, Nordström, K and Englund, P (1971) Resistance of Escherichia coli to penicillins. IX. Genetics and physiology of class II ampicillin-resistant mutants that are galactose negative or sensitive to bacteriophage C21, or both.J. Bacteriol. 108:1210-23
Abstract	Ampicillin-resistant mutants of class II are determined by a doubling of chromosomally and episomally mediated ampicillin resistance on agar plates. Several mutants were isolated from a female as well as from an Hfr strain. The mutants differed from each other in various properties such as response to colicin E2 and sodium cholate, response to the phages T4 and C21, and fermentation of galactose. By conjugation and transduction experiments, it was shown that mutations in at least four loci gave the class II phenotype. The mutations were found to be in the galU gene, the ctr gene, and two new genes close to mtl denoted lpsA and lpsB. The carbohydrate compositions of the lipopolysaccharides of the mutants were investigated and found to be changed compared to the parent strains. GalU mutants lacked rhamnose and galactose and had 11% glucose compared to the parent strain. The lpsA mutant also lacked rhamnose and had only traces of galactose and 58% glucose, whereas the lpsB mutant contained 14% rhamnose, traces of galactose, and 81% glucose compared to the parent strain.
Links	PubMed
Keywords	Ampicillin; Carbon Isotopes; Cell Wall; Chromosome Mapping; Conjugation, Genetic; Crosses, Genetic; Escherichia coli; Fermentation; Galactose; Glucose; Isomerases; Lipopolysaccharides; Lysogeny; Mutation; Penicillin Resistance; Phenotype; Phosphoglucomutase; Rhamnose; Salmonella Phages; Transduction, Genetic
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Main Points of the Paper

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Materials and Methods Used

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Phenotype Annotations

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Phenotype of	Taxon Information	Genotype Information (if known)	Condition Information	OMP ID	OMP Term Name	ECO ID	ECO Term Name	Notes	Status
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: D21e18 NCBI Taxon ID: 83333	Genotype of Reference Strain: ptsI Genotype of Experimental Strain : ptsI	Reference Condition:					Galactose negative (auxotrophic), table 7
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: D21e18 NCBI Taxon ID: 83333	Genotype of Reference Strain: ptsI Genotype of Experimental Strain : ptsI	Reference Condition:					resistant to phage C21
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: D21e18 NCBI Taxon ID: 83333	Genotype of Reference Strain: ptsI Genotype of Experimental Strain : ptsI	Reference Condition:					Was unable to grown in minimal medium with glucose as sole carbon source. Table 2
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: D21 NCBI Taxon ID: 83333	Genotype of Reference Strain: ptsI Genotype of Experimental Strain : ptsI in mutant strain D21e18	Reference Condition:					Ampicillin resistance in LA plates containing ampicillin.
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: D21 NCBI Taxon ID: 83333	Genotype of Reference Strain: galU Genotype of Experimental Strain : galU in mutant strain D21e8	Reference Condition:					Ampicillin resistance in LA plates containing ampicillin.
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: D21 NCBI Taxon ID: 83333	Genotype of Reference Strain: lpsA Genotype of Experimental Strain : lpsA in mutant strain D21e7	Reference Condition:					Ampicillin resistance in LA plates containing ampicillin.
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: D21 NCBI Taxon ID: 83333	Genotype of Reference Strain: lpsB Genotype of Experimental Strain : lpsB in mutant strain D21e19	Reference Condition:					Ampicillin resistance in LA plates containing ampicillin.
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