PMID:4945191

From OMPwiki
Jump to: navigation, search
Citation

Eriksson-Grennberg, KR, Nordström, K and Englund, P (1971) Resistance of Escherichia coli to penicillins. IX. Genetics and physiology of class II ampicillin-resistant mutants that are galactose negative or sensitive to bacteriophage C21, or both.J. Bacteriol. 108:1210-23

Abstract

Ampicillin-resistant mutants of class II are determined by a doubling of chromosomally and episomally mediated ampicillin resistance on agar plates. Several mutants were isolated from a female as well as from an Hfr strain. The mutants differed from each other in various properties such as response to colicin E2 and sodium cholate, response to the phages T4 and C21, and fermentation of galactose. By conjugation and transduction experiments, it was shown that mutations in at least four loci gave the class II phenotype. The mutations were found to be in the galU gene, the ctr gene, and two new genes close to mtl denoted lpsA and lpsB. The carbohydrate compositions of the lipopolysaccharides of the mutants were investigated and found to be changed compared to the parent strains. GalU mutants lacked rhamnose and galactose and had 11% glucose compared to the parent strain. The lpsA mutant also lacked rhamnose and had only traces of galactose and 58% glucose, whereas the lpsB mutant contained 14% rhamnose, traces of galactose, and 81% glucose compared to the parent strain.

Links

PubMed

Keywords

Ampicillin; Carbon Isotopes; Cell Wall; Chromosome Mapping; Conjugation, Genetic; Crosses, Genetic; Escherichia coli; Fermentation; Galactose; Glucose; Isomerases; Lipopolysaccharides; Lysogeny; Mutation; Penicillin Resistance; Phenotype; Phosphoglucomutase; Rhamnose; Salmonella Phages; Transduction, Genetic

Main Points of the Paper

Please summarize the main points of the paper.

Materials and Methods Used

Please list the materials and methods used in this paper (strains, plasmids, antibodies, etc).

Phenotype Annotations

See Help:AnnotationTable for details on how to edit this table.

Phenotype of Taxon Information Genotype Information (if known) Condition Information OMP ID OMP Term Name ECO ID ECO Term Name Notes Status

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: D21
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: galU+
  • Genotype of Experimental Strain : galU in mutant strain D21e8
  • Reference Condition:

Unable to utilize carbon source galactose, table 8

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: D21e18
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: ptsI
  • Genotype of Experimental Strain : ptsI
  • Reference Condition:

resistant to phage C21

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: D21e18
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: ptsI
  • Genotype of Experimental Strain : ptsI
  • Reference Condition:

Was unable to grown in minimal medium with glucose as sole carbon source. Table 2

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: D21
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: ptsI
  • Genotype of Experimental Strain : ptsI in mutant strain D21e18
  • Reference Condition:
OMP:0000276

increased resistance to antimicrobial compound

Ampicillin resistance in LA plates containing ampicillin.

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: D21
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: galU
  • Genotype of Experimental Strain : galU in mutant strain D21e8
  • Reference Condition:
OMP:0000276

increased resistance to antimicrobial compound

Ampicillin resistance in LA plates containing ampicillin.

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: D21
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: lpsA
  • Genotype of Experimental Strain : lpsA in mutant strain D21e7
  • Reference Condition:
OMP:0000276

increased resistance to antimicrobial compound

Ampicillin resistance in LA plates containing ampicillin.

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: D21
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: lpsB
  • Genotype of Experimental Strain : lpsB in mutant strain D21e19
  • Reference Condition:
OMP:0000276

increased resistance to antimicrobial compound

Ampicillin resistance in LA plates containing ampicillin.

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: D21
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: galU
  • Genotype of Experimental Strain : galU in mutant strain D21e8
  • Reference Condition:

Sensitive to sodium cholate, table 2

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: D21
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: galU
  • Genotype of Experimental Strain : galU in mutant strain D21e8
  • Reference Condition:

Sensitive to phage T4, table 2

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: D21
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: galU
  • Genotype of Experimental Strain : galU in mutant strain D21e8
  • Reference Condition:

Sensitive to phage C21, table 2. D21e8 and D21e10 contain a deletion covering galU gene.

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: D21
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: galU
  • Genotype of Experimental Strain : galU in mutant strain D21e8
  • Reference Condition:

Sensitive to phage P1bt, table 2


Notes

References

See Help:References for how to manage references in OMPwiki.