Difference between revisions of "PMID:14153532"
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*Taxon: Escherichia coli | *Taxon: Escherichia coli | ||
*Strain: K-12 | *Strain: K-12 | ||
| − | *Substrain: | + | *Substrain: z-15 |
*NCBI Taxon ID: [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&id=83333&lvl=3&p=mapview&p=has_linkout&p=blast_url&p=genome_blast&lin=f&keep=1&srchmode=1&unlock 83333] | *NCBI Taxon ID: [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&id=83333&lvl=3&p=mapview&p=has_linkout&p=blast_url&p=genome_blast&lin=f&keep=1&srchmode=1&unlock 83333] | ||
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| − | *Genotype of Reference Strain: | + | *Genotype of Reference Strain: Strain z-15 |
| + | *Genotype of Experimental Strain : Derivatives of Z-15 | ||
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*Reference Condition: | *Reference Condition: | ||
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| − | + | Abolished utilization of carbon source lactose in mutant strain. See Table 1. | |
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Revision as of 16:56, 18 April 2013
| Citation |
WITKIN, EM and SICURELLA, NA (1964) PURE CLONES OF LACTOSE-NEGATIVE MUTANTS OBTAINED IN ESCHERICHIA COLI AFTER TREATMENT WITH 5-BROMOURACIL. J. Mol. Biol. 8:610-3 |
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| Abstract |
No abstract in PubMed |
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| Keywords |
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Main Points of the Paper
Please summarize the main points of the paper.
- Describes the introduction of mutations (by incorporation of 5BU into DNA) that results in cells unable to ferment lactose
- Sectoring phenotypes appear, indicative of the timing the mutation gets incorporated within a cell of a colony
Materials and Methods Used
Please list the materials and methods used in this paper (strains, plasmids, antibodies, etc).
- Parent strain: Z-15 (radiation-resistant, serine-requiring variant of the thymineless strain B3)
- 5-Bromouracil Mutagenesis (on plates)
- strain plated on 5BU completed 1 round of replication, whereby most of the thymine was replaced by 5BU
- strain plated on thymine (control) completed a normal round of DNA replication
- Bacto-eosin-methylene blue (EMB) indicator agar (for more information, click here)
- dark red colonies (lactose +)
- colorless colonies (lactose -)
- mixed
- large sectors (lac- component originated at the center and constituted one-eighth or more the area of the colony)
- peripheral sectors
Phenotype Annotations
See Help:AnnotationTable for details on how to edit this table.
| Phenotype of | Taxon Information | Genotype Information (if known) | Condition Information | OMP ID | OMP Term Name | ECO ID | ECO Term Name | Notes | Status |
|---|---|---|---|---|---|---|---|---|---|
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a mutation or genetic difference within a strain |
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Abolished utilization of carbon source lactose in mutant strain. See Table 1. |
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Notes
References
See Help:References for how to manage references in OMPwiki.
