PMID:14153532

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Citation

WITKIN, EM and SICURELLA, NA (1964) PURE CLONES OF LACTOSE-NEGATIVE MUTANTS OBTAINED IN ESCHERICHIA COLI AFTER TREATMENT WITH 5-BROMOURACIL. J. Mol. Biol. 8:610-3

Abstract

No abstract in PubMed

Links

PubMed

Keywords


Main Points of the Paper

Please summarize the main points of the paper.

  • Describes the introduction of mutations (by incorporation of 5BU into DNA) that results in cells unable to ferment lactose
  • Sectoring phenotypes appear, indicative of the timing the mutation gets incorporated within a cell of a colony

Materials and Methods Used

Please list the materials and methods used in this paper (strains, plasmids, antibodies, etc).

  • Parent strain: Z-15 (radiation-resistant, serine-requiring variant of the thymineless strain B3)
  • 5-Bromouracil Mutagenesis (on plates)
    • strain plated on 5BU completed 1 round of replication, whereby most of the thymine was replaced by 5BU
    • strain plated on thymine (control) completed a normal round of DNA replication
  • Bacto-eosin-methylene blue (EMB) indicator agar (for more information, click here)
    • dark red colonies (lactose +)
    • colorless colonies (lactose -)
    • mixed
      • large sectors (lac- component originated at the center and constituted one-eighth or more the area of the colony)
      • peripheral sectors

Phenotype Annotations

See Help:AnnotationTable for details on how to edit this table.

Phenotype of Taxon Information Genotype Information (if known) Condition Information OMP ID OMP Term Name ECO ID ECO Term Name Notes Status

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: z-15
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: Strain z-15
  • Genotype of Experimental Strain : Derivatives of Z-15
  • Reference Condition:

OMP:0000284

loss of utilization of carbon-containing compound

ECO:0000178

in vivo assay evidence

Abolished utilization of carbon source lactose in mutant strain. See Table 1.


Notes

OMP: After carbon utilization terms get finialized, make sure OMP ID matches what is on here since it might change.

ECO: Terms for assays are awaiting implementation of solid and liquid child terms to each one. Experiment used EMB

References

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