OMP ST:71482 ! Escherichia coli K-12 JW0429

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Strain Summary

Strain Name

Escherichia coli K-12 JW0429

Synonyms

JW0429-1

Taxon Information
  • Pangenome: Escherichia coli
  • Subspecies and/or strain: K-12
  • NCBI Taxonomy ID: 83333
Genotype

DE(araD-araB)567 DE(rhaD-rhaB)568 F- Lambda- hsdR514 lacZ4787(del)(::rrnB-3) rph-1 lon-725(del)::FRT-kan-FRT

Strain Reference
Strain availability

Coli Genetic Stock Center

Ancestry

parent:OMP ST:800 ! Escherichia coli K-12 BW25113

Annotated phenotypes



Notes

Phenotype Annotation

Annotation ID Qualifier OMP ID OMP term name Relative phenotype information Experimental condition ECO ID ECO term name Reference Annotation Extension Notes

OMP_AN:28442

OMP:0007959

increased population growth at low temperature

Relative to: OMP_AN:27620


Genotype differences:

+lacZ4787(del)( +lon-725(del) -lacZ4787(del)


Condition differences:
  • temperature:16C
  • medium37C
  • medium
  • temperature:16C
  • medium:LB agar
  • medium:Low temperature
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

  • chemical concentration= 16 C
  • normalized fitness= 7.91

OMP_AN:28443

OMP:0006044

decreased resistance to beta-lactam

Relative to: OMP_AN:27531


Genotype differences:

+lacZ4787(del)( +lon-725(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Ampicillin
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:28971) Ampicillin

  • chemical concentration= 8 ug/ml
  • normalized fitness= -3.32

OMP_AN:28444

OMP:0007895

increased resistance to a surfactant

Relative to: OMP_AN:27581


Genotype differences:

+lacZ4787(del)( +lon-725(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Benzalkonium chloride
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:3020) Benzalkonium chloride

  • chemical concentration= 10 ug/ml
  • normalized fitness= 5.17

OMP_AN:28445

OMP:0007878

increased resistance to an antimicrobial agent

Relative to: OMP_AN:27556


Genotype differences:

+lacZ4787(del)( +lon-725(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Bicyclomycin
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:60584) Bicyclomycin

  • chemical concentration= 1 ug/ml
  • normalized fitness= 4.96

OMP_AN:28446

OMP:0006044

decreased resistance to beta-lactam

Relative to: OMP_AN:27534


Genotype differences:

+lacZ4787(del)( +lon-725(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Cefoxitin sodium hydrate
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:3501) Cefoxitin sodium hydrate

  • chemical concentration= 0.25 ug/ml
  • normalized fitness= -3.68

OMP_AN:28447

OMP:0007249

decreased resistance to a chemical

Relative to: OMP_AN:27592


Genotype differences:

+lacZ4787(del)( +lon-725(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Calcofluor White M2R (F3543 Fluorescent Brightener 28)
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:50010) Calcofluor White M2R (F3543 Fluorescent Brightener 28)

  • chemical concentration= 50 ug/ml
  • normalized fitness= -3.81

OMP_AN:28448

OMP:0006044

decreased resistance to beta-lactam

Relative to: OMP_AN:27535


Genotype differences:

+lacZ4787(del)( +lon-725(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Cefsulodin sodium salt hydrate
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:31380) Cefsulodin sodium salt hydrate

  • chemical concentration= 12 ug/ml
  • normalized fitness= -3.49

OMP_AN:28449

OMP:0007888

increased resistance to a metal

Relative to: OMP_AN:27579


Genotype differences:

+lacZ4787(del)( +lon-725(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Copper stress-CuCl2 dihydrate
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:86318) Copper stress-CuCl2 dihydrate

  • chemical concentration= 4 mM (LB)
  • normalized fitness= 3.29

OMP_AN:28450

OMP:0007903

decreased resistance to bile acids

Relative to: OMP_AN:27586


Genotype differences:

+lacZ4787(del)( +lon-725(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Deoxycholate sodium
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:9177) Deoxycholate sodium

  • chemical concentration= 0.5 %
  • normalized fitness= -5.54

OMP_AN:28451

OMP:0007902

increased resistance to bile acids

Relative to: OMP_AN:27586


Genotype differences:

+lacZ4787(del)( +lon-725(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Deoxycholate sodium
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:9177) Deoxycholate sodium

  • chemical concentration= 2 %
  • normalized fitness= 3.17

OMP_AN:28452

OMP:0007879

decreased resistance to an antimicrobial agent

Relative to: OMP_AN:27515


Genotype differences:

+lacZ4787(del)( +lon-725(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Fusidic acid sodium salt
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CID:126963539) Fusidic acid sodium salt

  • chemical concentration= 1 ug/ml
  • normalized fitness= -4.4

OMP_AN:28453

OMP:0007074

increased growth factor requirement

Relative to: OMP_AN:27606


Genotype differences:

+lacZ4787(del)( +lon-725(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:M9
  • medium:N-Acetyl-D-glucosamine
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:506227) N-Acetyl-D-glucosamine

  • chemical concentration= 0.15%
  • normalized fitness= 7.07

OMP_AN:28454

OMP:0007071

increased organic carbon source utilization

Relative to: OMP_AN:27616


Genotype differences:

+lacZ4787(del)( +lon-725(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:M9
  • medium:N-Acetyl-D-glucosamine
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:506227) N-Acetyl-D-glucosamine

  • chemical concentration= 0.15%
  • normalized fitness= 7.07

OMP_AN:28455

OMP:0007074

increased growth factor requirement

Relative to: OMP_AN:27602


Genotype differences:

+lacZ4787(del)( +lon-725(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:M9
  • medium:D-(+) Glucosamine hydrochloride
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CID:91431) D-(+) Glucosamine hydrochloride

  • chemical concentration= 0.2%
  • normalized fitness= 3.83

OMP_AN:28456

OMP:0007071

increased organic carbon source utilization

Relative to: OMP_AN:27612


Genotype differences:

+lacZ4787(del)( +lon-725(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:M9
  • medium:D-(+) Glucosamine hydrochloride
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CID:91431) D-(+) Glucosamine hydrochloride

  • chemical concentration= 0.2%
  • normalized fitness= 3.83

OMP_AN:28457

OMP:0007074

increased growth factor requirement

Relative to: OMP_AN:27603


Genotype differences:

+lacZ4787(del)( +lon-725(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:M9
  • medium:D-Glucose anhydrous
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:42758) D-Glucose anhydrous

  • chemical concentration= 0.2%
  • normalized fitness= 5.61

OMP_AN:28458

OMP:0007071

increased organic carbon source utilization

Relative to: OMP_AN:27613


Genotype differences:

+lacZ4787(del)( +lon-725(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:M9
  • medium:D-Glucose anhydrous
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:42758) D-Glucose anhydrous

  • chemical concentration= 0.2%
  • normalized fitness= 5.61

OMP_AN:28459

OMP:0007074

increased growth factor requirement

Relative to: OMP_AN:27604


Genotype differences:

+lacZ4787(del)( +lon-725(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:M9
  • medium:Glycerol
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:17754) Glycerol

  • chemical concentration= 0.4%
  • normalized fitness= 7.51

OMP_AN:28460

OMP:0007071

increased organic carbon source utilization

Relative to: OMP_AN:27614


Genotype differences:

+lacZ4787(del)( +lon-725(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:M9
  • medium:Glycerol
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:17754) Glycerol

  • chemical concentration= 0.4%
  • normalized fitness= 7.51

OMP_AN:28461

OMP:0007878

increased resistance to an antimicrobial agent

Relative to: OMP_AN:27545


Genotype differences:

+lacZ4787(del)( +lon-725(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Isoniazid
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:6030) Isoniazid

  • chemical concentration= 1 mM
  • normalized fitness= 4.26

OMP_AN:28462

OMP:0007376

increased resistance to an alkylating agent

Relative to: OMP_AN:27502


Genotype differences:

+lacZ4787(del)( +lon-725(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:methyl methanesulfonate (MMS)
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:25255) methyl methanesulfonate (MMS)

  • chemical concentration= 0.05%
  • normalized fitness= 6.39

OMP_AN:28463

OMP:0007074

increased growth factor requirement

Relative to: OMP_AN:27605


Genotype differences:

+lacZ4787(del)( +lon-725(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:M9
  • medium:D-(+) Maltose monohydrate
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CID:23615261) D-(+) Maltose monohydrate

  • chemical concentration= 0.1%
  • normalized fitness= 7.74

OMP_AN:28464

OMP:0007071

increased organic carbon source utilization

Relative to: OMP_AN:27615


Genotype differences:

+lacZ4787(del)( +lon-725(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:M9
  • medium:D-(+) Maltose monohydrate
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CID:23615261) D-(+) Maltose monohydrate

  • chemical concentration= 0.1%
  • normalized fitness= 7.74

OMP_AN:28465

OMP:0007074

increased growth factor requirement

Relative to: OMP_AN:27600


Genotype differences:

+lacZ4787(del)( +lon-725(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:MOPS
  • medium:NH4Cl
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:31206) NH4Cl

  • chemical concentration= 9.5mm
  • normalized fitness= 5.97

OMP_AN:28466

OMP:0007033

increased utilization of inorganic nitrogen source

Relative to: OMP_AN:27610


Genotype differences:

+lacZ4787(del)( +lon-725(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:MOPS
  • medium:NH4Cl
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:31206) NH4Cl

  • chemical concentration= 9.5mm
  • normalized fitness= 5.97

OMP_AN:28467

OMP:0007888

increased resistance to a metal

Relative to: OMP_AN:27580


Genotype differences:

+lacZ4787(del)( +lon-725(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Nickel stress-Ni(II)Cl2 hexahydrate
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:53542) Nickel stress-Ni(II)Cl2 hexahydrate

  • chemical concentration= 1 mM (LB)
  • normalized fitness= 4.03

OMP_AN:28468

OMP:0007878

increased resistance to an antimicrobial agent

Relative to: OMP_AN:27588


Genotype differences:

+lacZ4787(del)( +lon-725(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Triclosan/Irgasan
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:164200) Triclosan/Irgasan

  • chemical concentration= 0.05 ug/ml
  • normalized fitness= 5.96

OMP_AN:28469

OMP:0007895

increased resistance to a surfactant

Relative to: OMP_AN:27583


Genotype differences:

+lacZ4787(del)( +lon-725(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Triton X-100
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:9750) Triton X-100

  • chemical concentration= 0.2 %
  • normalized fitness= 3.81

OMP_AN:28470

OMP:0006044

decreased resistance to beta-lactam

Relative to: OMP_AN:27532


Genotype differences:

+lacZ4787(del)( +lon-725(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Aztreonam
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:161680) Aztreonam

  • chemical concentration= 0.02 ug/ml
  • normalized fitness= -4.69

OMP_AN:28471

OMP:0006041

increased resistance to quinolone

Relative to: OMP_AN:27539


Genotype differences:

+lacZ4787(del)( +lon-725(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Ciprofloxacin hydrochloride hydrate
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:59936) Ciprofloxacin hydrochloride hydrate

  • chemical concentration= 0.008 ug/ml
  • normalized fitness= 4.95

OMP_AN:28472

OMP:0006063

increased resistance to tetracycline

Relative to: OMP_AN:27522


Genotype differences:

+lacZ4787(del)( +lon-725(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Doxycycline hyclate
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:34730) Doxycycline hyclate

  • chemical concentration= 0.75 ug/ml
  • normalized fitness= 3.22

OMP_AN:28473

OMP:0007074

increased growth factor requirement

Relative to: OMP_AN:27598


Genotype differences:

+lacZ4787(del)( +lon-725(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:MOPS
  • medium:Iron excess-FeSO4 heptahydride
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:75836) Iron excess-FeSO4 heptahydride

  • chemical concentration= 1mM (MOPS; normal 100uM)
  • normalized fitness= 5.75

OMP_AN:28474

OMP:0007888

increased resistance to a metal

Relative to: OMP_AN:27608


Genotype differences:

+lacZ4787(del)( +lon-725(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:MOPS
  • medium:Iron excess-FeSO4 heptahydride
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:75836) Iron excess-FeSO4 heptahydride

  • chemical concentration= 1mM (MOPS; normal 100uM)
  • normalized fitness= 5.75

OMP_AN:28475

OMP:0007074

increased growth factor requirement

Relative to: OMP_AN:27599


Genotype differences:

+lacZ4787(del)( +lon-725(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:MOPS
  • medium:Iron starvation-FeSO4·7H2O
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:75836) Iron starvation-FeSO4·7H2O

  • chemical concentration= 2uM (MOPS; normal 100uM)
  • normalized fitness= 5.31

OMP_AN:28476

OMP:0007936

increased resistance to nutrient limitation

Relative to: OMP_AN:27609


Genotype differences:

+lacZ4787(del)( +lon-725(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:MOPS
  • medium:Iron starvation-FeSO4·7H2O
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:75836) Iron starvation-FeSO4·7H2O

  • chemical concentration= 2uM (MOPS; normal 100uM)
  • normalized fitness= 5.31

OMP_AN:28477

OMP:0006041

increased resistance to quinolone

Relative to: OMP_AN:27542


Genotype differences:

+lacZ4787(del)( +lon-725(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Norfloxacin
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:100246) Norfloxacin

  • chemical concentration= 0.04 ug/ml
  • normalized fitness= 3.4

OMP_AN:28478

OMP:0007008

decreased population growth at acidic pH

Relative to: OMP_AN:27622


Genotype differences:

+lacZ4787(del)( +lon-725(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:MES-HOMOPIPES
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (**SID:329817955 SID:57651422) MES-HOMOPIPES

  • chemical concentration= pH 4
  • normalized fitness= -6.74

OMP_AN:28479

OMP:0007009

increased population growth at acidic pH

Relative to: OMP_AN:27622


Genotype differences:

+lacZ4787(del)( +lon-725(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:MES-HOMOPIPES
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (**SID:329817955 SID:57651422) MES-HOMOPIPES

  • chemical concentration= 6
  • normalized fitness= 6.88

OMP_AN:28480

OMP:0007878

increased resistance to an antimicrobial agent

Relative to: OMP_AN:27565


Genotype differences:

+lacZ4787(del)( +lon-725(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Polymyxin B sulfate
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:8310) Polymyxin B sulfate

  • chemical concentration= 6 ug/ml
  • normalized fitness= 6.51

OMP_AN:28481

OMP:0006037

increased resistance to macrolide

Relative to: OMP_AN:27521


Genotype differences:

+lacZ4787(del)( +lon-725(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Spiramycin
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CID:6419898) Spiramycin

  • chemical concentration= 5 ug/ml
  • normalized fitness= 3.54

OMP_AN:28482

OMP:0007832

increased resistance to sulfonamide

Relative to: OMP_AN:27576


Genotype differences:

+lacZ4787(del)( +lon-725(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Sulfamethizole
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:9331) Sulfamethizole

  • chemical concentration= 100 ug/ml
  • normalized fitness= 4.48


Additional Information

Links

References

See Help:References for how to manage references in OMPwiki.

  1. ↑ Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  2. ↑ 2.00 2.01 2.02 2.03 2.04 2.05 2.06 2.07 2.08 2.09 2.10 2.11 2.12 2.13 2.14 2.15 2.16 2.17 2.18 2.19 2.20 2.21 2.22 2.23 2.24 2.25 2.26 2.27 2.28 2.29 2.30 2.31 2.32 2.33 2.34 2.35 2.36 2.37 2.38 2.39 2.40 Nichols, RJ et al. (2011) Phenotypic landscape of a bacterial cell. Cell 144 143-56 PubMed