id: ECO:0001829
name: ferric uptake regulator titration assay evidence
def: "A type of reporter gene assay evidence used to locate and isolate Fur sites where a plasmid library is created and cloned into a cell line with a ferric uptake regulator (Fur)-repressed reporter and the Fur protein is titrated away from its binding site on the reporter, after which the cell is isolated and plasmid extracted for further sequence analysis." [ECO:SW, PMID:10713425, PMID:7642488, PMID:8107138]
synonym: "Fluorescence polarization" RELATED [PMID:15689115]
synonym: "FURTA" EXACT [PMID:10713425]
is_a: ECO:0000049 ! reporter gene assay evidence
relationship: OBI:0000312: OBI:0000913 ! is_specified_output_of promoter activity detection by reporter gene assay
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Last version checked
releases/2019-06-14.
14:06:2019 08:25.
rctauber | Last updated
releases/2019-06-14 | |
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