Category:Bromothymol Blue indicator plates

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Purpose

  • Allows for more sensitive detection of media acidification using lower carbohydrate concentrations (compared to other standard indicator plates)

Technical Details

Materials Needed

  • Ingredients[1]
    • Tryptone
    • NaCl
    • Bromothymol blue (BTB) solution
    • Bile salts no. 3
    • Agar
    • Sugar (L-arabinose, L-rhamnose, D-lactose, and D-galactose were used in the reference)

Methods

Examples

Notes

References

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  1. ↑ DuPree, MR & Wilcox, G (1977) Bromothymol blue and carbohydrate-sensitive plating media. J. Clin. Microbiol. 6 343-7 PubMed

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