OMP ST:74667 ! Virtual reference strain for Nichols RJ (2011) Cell 144, 143-156.
Virtual reference strain for Nichols RJ (2011) Cell 144, 143-156.
DE(araD-araB)567 DE(rhaD-rhaB)568 F- Lambda- hsdR514 lacZ4787(del)::rrnB-3 rph-1
OMP_ST:74667 was created to use as the reference (or control) strain for the high-throughput phenotype studies of Nichols RJ et al. (2011) Cell 144:143-156. In this study, fitness in the various growth conditions was determined based on the sizes of spot colonies. Colony sizes were normalized in two steps. First, colony sizes on each individual plate were normalized to correct for systematic differences. Second, the colony sizes for each strain were normalized based on the median colony size of that strain in all 324 conditions.
Of the 4,602 strains that were screened, 4,502 strains were derivatives of E. coli K-12 BW25113; the remaining 100 strains were derivative of E. coli K-12 MG1655. For this reason, we have used the genotype of BW25113 as the genotype for the virtual reference strain.
The collection of mutants that was screened included 4334 deletions of non-essential genes (the Keio Collection (), 148 SPA-tagged derivatives of essential genes ( ), 9 point-mutant alleles of essential genes (as well as the corresponding control strains that carried the same antibioticR marker linked to the wild-type allele), 2 truncations of essential genes, and 100 deletions of small RNAs and small proteins (). See the original paper for additional details about the strains used in the screen.
|Annotation ID||Qualifier||OMP ID||OMP term name||Relative phenotype information||Experimental condition||ECO ID||ECO term name||Reference||Annotation Extension||Notes|
resistant to a chemical
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high throughput direct assay evidence used in manual assertion
towards some (CHEBI:8984) SDS
concentrations of SDS used were (0.5, 1, 2, 3, or 4%)
See Help:References for how to manage references in OMPwiki.
- Nichols, RJ et al. (2011) Phenotypic landscape of a bacterial cell. Cell 144 143-56 PubMed OMPwiki page
- Hobbs, EC et al. (2010) Small RNAs and small proteins involved in resistance to cell envelope stress and acid shock in Escherichia coli: analysis of a bar-coded mutant collection. J. Bacteriol. 192 59-67 PubMed OMPwiki page