Difference between revisions of "OMP ST:71354 ! Escherichia coli K-12 JW0233"

Revision as of 19:22, 7 June 2021

Strain Summary

Strain Name	Escherichia coli K-12 JW0233
Synonyms	JW0233-2
Taxon Information	Pangenome: Escherichia coli Subspecies and/or strain: K-12 NCBI Taxonomy ID: 83333
Genotype	DE(araD-araB)567 DE(rhaD-rhaB)568 F- Lambda- hsdR514 lacZ4787(del)::rrnB-3 rph-1 proA761(del)::FRT-kan-FRT
Strain Reference	Coli Genetic Stock Center PMID:16738554^[1]
Strain availability	Coli Genetic Stock Center
Ancestry	parent:OMP ST:800 ! Escherichia coli K-12 BW25113
Annotated phenotypes
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Notes

Phenotype Annotation

Annotation ID	OMP ID	OMP term name	Relative phenotype information	Experimental condition	ECO ID	ECO term name	Reference	Annotation Extension	Notes
OMP_AN:28211	OMP:0007074	increased growth factor requirement	Relative to: OMP_AN:27601 Genotype differences: +proA761(del) Condition differences: no differences	temperature:37C medium:M9 medium: Sodium Acetate (0.6%)	ECO:0007005	high throughput direct assay evidence used in manual assertion	PMID:21185072^[2]		normalized fitness= -9.5
OMP_AN:28213	OMP:0007074	increased growth factor requirement	Relative to: OMP_AN:27606 Genotype differences: +proA761(del) Condition differences: no differences	temperature:37C medium:M9 medium:N-Acetyl-D-glucosamine (0.15%)	ECO:0007005	high throughput direct assay evidence used in manual assertion	PMID:21185072^[2]		normalized fitness= -15.59
OMP_AN:28215	OMP:0007074	increased growth factor requirement	Relative to: OMP_AN:27602 Genotype differences: +proA761(del) Condition differences: no differences	temperature:37C medium:M9 medium:D-(+) Glucosamine hydrochloride (0.2%)	ECO:0007005	high throughput direct assay evidence used in manual assertion	PMID:21185072^[2]		(CID:91431) D-(+) Glucosamine hydrochloride= 0.2% normalized fitness= -15.4
OMP_AN:28217	OMP:0007074	increased growth factor requirement	Relative to: OMP_AN:27603 Genotype differences: +proA761(del) Condition differences: no differences	temperature:37C medium:M9 medium:D-Glucose (0.2%)	ECO:0007005	high throughput direct assay evidence used in manual assertion	PMID:21185072^[2]	towards some (CHEBI:42758) D-Glucose anhydrous	chemical concentration= 0.2% normalized fitness= -16.79
OMP_AN:28219	OMP:0007074	increased growth factor requirement	Relative to: OMP_AN:27604 Genotype differences: +proA761(del) Condition differences: no differences	temperature:37C medium:M9 medium:Glycerol (0.4%)	ECO:0007005	high throughput direct assay evidence used in manual assertion	PMID:21185072^[2]		normalized fitness= -20.78
OMP_AN:28221	OMP:0007074	increased growth factor requirement	Relative to: OMP_AN:27605 Genotype differences: +proA761(del) Condition differences: no differences	temperature:37C medium:M9 medium:D-(+) Maltose (0.1%)	ECO:0007005	high throughput direct assay evidence used in manual assertion	PMID:21185072^[2]		normalized fitness= -15.46
OMP_AN:28223	OMP:0007074	increased growth factor requirement	Relative to: OMP_AN:27600 Genotype differences: +proA761(del) Condition differences: no differences	temperature:37C medium:MOPS medium:NH4Cl (9.5 mM)	ECO:0007005	high throughput direct assay evidence used in manual assertion	PMID:21185072^[2]		normalized fitness= -23.94
OMP_AN:28225	OMP:0007074	increased growth factor requirement	Relative to: OMP_AN:27607 Genotype differences: +proA761(del) Condition differences: no differences	temperature:37C medium:M9 medium:Succinic acid (0.3%)	ECO:0007005	high throughput direct assay evidence used in manual assertion	PMID:21185072^[2]		normalized fitness= -10.5
OMP_AN:28226	OMP:0007074	increased growth factor requirement	Relative to: OMP_AN:27617 Genotype differences: +proA761(del) Condition differences: no differences	temperature:37C medium:M9 medium:Succinic acid	ECO:0007005	high throughput direct assay evidence used in manual assertion	PMID:21185072^[2]	towards some (CHEBI:15741) Succinic acid	chemical concentration= 0.3% normalized fitness= -10.5
OMP_AN:28227	OMP:0007074	increased growth factor requirement	Relative to: OMP_AN:27598 Genotype differences: +proA761(del) Condition differences: no differences	temperature:37C medium:MOPS medium:FeSO4 (1 mM, iron excess)	ECO:0007005	high throughput direct assay evidence used in manual assertion	PMID:21185072^[2]		chemical concentration= 1mM (MOPS; normal 100uM) normalized fitness= -24.76
OMP_AN:28228	OMP:0007886	decreased resistance to a metal	Relative to: OMP_AN:27608 Genotype differences: +proA761(del) Condition differences: no differences	temperature:37C medium:MOPS medium:Iron excess-FeSO4 heptahydride	ECO:0007005	high throughput direct assay evidence used in manual assertion	PMID:21185072^[2]	towards some (CHEBI:75836) Iron excess-FeSO4 heptahydride	chemical concentration= 1mM (MOPS; normal 100uM) normalized fitness= -24.76
OMP_AN:28229	OMP:0007075	decreased growth factor requirement	Relative to: OMP_AN:27599 Genotype differences: +proA761(del) Condition differences: no differences	temperature:37C medium:MOPS medium:Iron starvation-FeSO4·7H2O	ECO:0007005	high throughput direct assay evidence used in manual assertion	PMID:21185072^[2]	towards some (CHEBI:75836) Iron starvation-FeSO4·7H2O	chemical concentration= 2uM (MOPS; normal 100uM) normalized fitness= -13.42
OMP_AN:28230	OMP:0007937	decreased resistance to nutrient limitation	Relative to: OMP_AN:27609 Genotype differences: +proA761(del) Condition differences: no differences	temperature:37C medium:MOPS medium:Iron starvation-FeSO4·7H2O	ECO:0007005	high throughput direct assay evidence used in manual assertion	PMID:21185072^[2]	towards some (CHEBI:75836) Iron starvation-FeSO4·7H2O	chemical concentration= 2uM (MOPS; normal 100uM) normalized fitness= -13.42
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References

See Help:References for how to manage references in OMPwiki.

↑ Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed OMPwiki page
↑ ^2.00 ^2.01 ^2.02 ^2.03 ^2.04 ^2.05 ^2.06 ^2.07 ^2.08 ^2.09 ^2.10 ^2.11 ^2.12 Nichols, RJ et al. (2011) Phenotypic landscape of a bacterial cell. Cell 144 143-56 PubMed OMPwiki page

[PMID:16738554-1] Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed OMPwiki page

[PMID:21185072-2] 2.00 ^2.01 ^2.02 ^2.03 ^2.04 ^2.05 ^2.06 ^2.07 ^2.08 ^2.09 ^2.10 ^2.11 ^2.12 Nichols, RJ et al. (2011) Phenotypic landscape of a bacterial cell. Cell 144 143-56 PubMed OMPwiki page

[1]

[2]

Difference between revisions of "OMP ST:71354 ! Escherichia coli K-12 JW0233"

Revision as of 19:22, 7 June 2021

Contents

Strain Summary

Notes

Phenotype Annotation

Additional Information

Links

References

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@@ Line 87: / Line 87: @@
 *temperature:37C
 *medium:M9
-*medium:Sodium Acetate anhydrous
+*medium: Sodium Acetate (0.6%)
 |
 ECO:0007005
@@ Line 95: / Line 95: @@
 PMID:21185072
 |
-towards some (CHEBI:32954) Sodium Acetate anhydrous
 |
+normalized fitness= -9.5
-*chemical concentration= 0.6%
-*normalized fitness= -9.5
 |-
 |
@@ Line 106: / Line 104: @@
 |
-OMP:0007075
+OMP:0007074
 |
-decreased growth factor requirement
+increased growth factor requirement
 |
 Relative to: OMP_AN:27606
@@ Line 120: / Line 118: @@
 *temperature:37C
 *medium:M9
-*medium:N-Acetyl-D-glucosamine
+*medium:N-Acetyl-D-glucosamine (0.15%)
 |
 ECO:0007005
@@ Line 127: / Line 125: @@
 |
 PMID:21185072
-|
-towards some (CHEBI:506227) N-Acetyl-D-glucosamine
-|
-*chemical concentration= 0.15%
-*normalized fitness= -15.59
-|-
-|
-OMP_AN:28214
 |
 |
-OMP:0007070
+normalized fitness= -15.59
-|
-decreased organic carbon source utilization
-|
-Relative to: OMP_AN:27616
-<hr>Genotype differences:<br>
-+proA761(del)
-<hr>Condition differences:<br>
-no differences
-|
-*temperature:37C
-*medium:M9
-*medium:N-Acetyl-D-glucosamine
-|
-ECO:0007005
-|
-high throughput direct assay evidence used in manual assertion
-|
-PMID:21185072
-|
-towards some (CHEBI:506227) N-Acetyl-D-glucosamine
-|
-*chemical concentration= 0.15%
-*normalized fitness= -15.59
 |-
 |
@@ Line 172: / Line 135: @@
 |
-OMP:0007075
+OMP:0007074
 |
-decreased growth factor requirement
+increased growth factor requirement
 |
 Relative to: OMP_AN:27602
@@ Line 186: / Line 149: @@
 *temperature:37C
 *medium:M9
-*medium:D-(+) Glucosamine hydrochloride
+*medium:D-(+) Glucosamine hydrochloride (0.2%)
 |
 ECO:0007005
@@ Line 193: / Line 156: @@
 |
 PMID:21185072
-|
-towards some (CID:91431) D-(+) Glucosamine hydrochloride
-|
-*chemical concentration= 0.2%
-*normalized fitness= -15.4
-|-
-|
-OMP_AN:28216
-|
-|
-OMP:0007070
-|
-decreased organic carbon source utilization
-|
-Relative to: OMP_AN:27612
-<hr>Genotype differences:<br>
-+proA761(del)
-<hr>Condition differences:<br>
-no differences
 |
-*temperature:37C
-*medium:M9
-*medium:D-(+) Glucosamine hydrochloride
-|
-ECO:0007005
-|
-high throughput direct assay evidence used in manual assertion
-|
-PMID:21185072
-|
-towards some (CID:91431) D-(+) Glucosamine hydrochloride
 |
-*chemical concentration= 0.2%
+*(CID:91431) D-(+) Glucosamine hydrochloride= 0.2%
 *normalized fitness= -15.4
 |-
@@ Line 238: / Line 168: @@
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-OMP:0007075
+OMP:0007074
 |
-decreased growth factor requirement
+increased growth factor requirement
 |
 Relative to: OMP_AN:27603
@@ Line 252: / Line 182: @@
 *temperature:37C
 *medium:M9
-*medium:D-Glucose anhydrous
+*medium:D-Glucose (0.2%)
-|
-ECO:0007005
-|
-high throughput direct assay evidence used in manual assertion
-|
-PMID:21185072
-|
-towards some (CHEBI:42758) D-Glucose anhydrous
-|
-*chemical concentration= 0.2%
-*normalized fitness= -16.79
-|-
-|
-OMP_AN:28218
-|
-|
-OMP:0007070
-|
-decreased organic carbon source utilization
-|
-Relative to: OMP_AN:27613
-<hr>Genotype differences:<br>
-+proA761(del)
-<hr>Condition differences:<br>
-no differences
-|
-*temperature:37C
-*medium:M9
-*medium:D-Glucose anhydrous
 |
 ECO:0007005
@@ Line 304: / Line 201: @@
 |
-OMP:0007075
+OMP:0007074
 |
-decreased growth factor requirement
+increased growth factor requirement
 |
 Relative to: OMP_AN:27604
@@ Line 318: / Line 215: @@
 *temperature:37C
 *medium:M9
-*medium:Glycerol
+*medium:Glycerol (0.4%)
 |
 ECO:0007005
@@ Line 325: / Line 222: @@
 |
 PMID:21185072
-|
-towards some (CHEBI:17754) Glycerol
-|
-*chemical concentration= 0.4%
-*normalized fitness= -20.78
-|-
-|
-OMP_AN:28220
 |
 |
-OMP:0007070
+normalized fitness= -20.78
-|
-decreased organic carbon source utilization
-|
-Relative to: OMP_AN:27614
-<hr>Genotype differences:<br>
-+proA761(del)
-<hr>Condition differences:<br>
-no differences
-|
-*temperature:37C
-*medium:M9
-*medium:Glycerol
-|
-ECO:0007005
-|
-high throughput direct assay evidence used in manual assertion
-|
-PMID:21185072
-|
-towards some (CHEBI:17754) Glycerol
-|
-*chemical concentration= 0.4%
-*normalized fitness= -20.78
 |-
 |
@@ Line 370: / Line 232: @@
 |
-OMP:0007075
+OMP:0007074
 |
-decreased growth factor requirement
+increased growth factor requirement
 |
 Relative to: OMP_AN:27605
@@ Line 384: / Line 246: @@
 *temperature:37C
 *medium:M9
-*medium:D-(+) Maltose monohydrate
+*medium:D-(+) Maltose (0.1%)
 |
 ECO:0007005
@@ Line 392: / Line 254: @@
 PMID:21185072
 |
-towards some (CID:23615261) D-(+) Maltose monohydrate
-|
-*chemical concentration= 0.1%
-*normalized fitness= -15.46
-|-
-|
-OMP_AN:28222
-|
-|
-OMP:0007070
-|
-decreased organic carbon source utilization
-|
-Relative to: OMP_AN:27615
-<hr>Genotype differences:<br>
-+proA761(del)
-<hr>Condition differences:<br>
-no differences
-|
-*temperature:37C
-*medium:M9
-*medium:D-(+) Maltose monohydrate
-|
-ECO:0007005
-|
-high throughput direct assay evidence used in manual assertion
-|
-PMID:21185072
-|
-towards some (CID:23615261) D-(+) Maltose monohydrate
 |
+normalized fitness= -15.46
-*chemical concentration= 0.1%
-*normalized fitness= -15.46
 |-
 |
@@ Line 436: / Line 263: @@
 |
-OMP:0007075
+OMP:0007074
 |
-decreased growth factor requirement
+increased growth factor requirement
 |
 Relative to: OMP_AN:27600
@@ Line 450: / Line 277: @@
 *temperature:37C
 *medium:MOPS
-*medium:NH4Cl
+*medium:NH4Cl (9.5 mM)
 |
 ECO:0007005
@@ Line 457: / Line 284: @@
 |
 PMID:21185072
-|
-towards some (CHEBI:31206) NH4Cl
 |
-*chemical concentration= 9.5mm
-*normalized fitness= -23.94
-|-
 |
-OMP_AN:28224
+normalized fitness= -23.94
-|
-|
-OMP:0007032
-|
-decreased utilization of inorganic nitrogen source
-|
-Relative to: OMP_AN:27610
-<hr>Genotype differences:<br>
-+proA761(del)
-<hr>Condition differences:<br>
-no differences
-|
-*temperature:37C
-*medium:MOPS
-*medium:NH4Cl
-|
-ECO:0007005
-|
-high throughput direct assay evidence used in manual assertion
-|
-PMID:21185072
-|
-towards some (CHEBI:31206) NH4Cl
-|
-*chemical concentration= 9.5mm
-*normalized fitness= -23.94
 |-
 |
@@ Line 502: / Line 294: @@
 |
-OMP:0007075
+OMP:0007074
 |
-decreased growth factor requirement
+increased growth factor requirement
 |
 Relative to: OMP_AN:27607
@@ Line 516: / Line 308: @@
 *temperature:37C
 *medium:M9
-*medium:Succinic acid
+*medium:Succinic acid (0.3%)
 |
 ECO:0007005
@@ Line 524: / Line 316: @@
 PMID:21185072
 |
-towards some (CHEBI:15741) Succinic acid
 |
+normalized fitness= -10.5
-*chemical concentration= 0.3%
-*normalized fitness= -10.5
 |-
 |
@@ Line 568: / Line 358: @@
 |
-OMP:0007075
+OMP:0007074
 |
-decreased growth factor requirement
+increased growth factor requirement
 |
 Relative to: OMP_AN:27598
@@ Line 582: / Line 372: @@
 *temperature:37C
 *medium:MOPS
-*medium:Iron excess-FeSO4 heptahydride
+*medium:FeSO4 (1 mM, iron excess)
 |
 ECO:0007005
@@ Line 590: / Line 380: @@
 PMID:21185072
 |
-towards some (CHEBI:75836) Iron excess-FeSO4 heptahydride
 |