Difference between revisions of "OMP ST:71245 ! Escherichia coli K-12 JW0052"

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*chemical concentration= 0.1 ug/ml
 
*chemical concentration= 0.1 ug/ml
 
*normalized fitness= -8.77  
 
*normalized fitness= -8.77  
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OMP_AN:27869
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OMP:0007879
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decreased resistance to an antimicrobial agent
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Relative to: OMP_AN:27553
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<hr>Genotype differences:<br>
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+lacZ4787(del)( +surA765(del)  -lacZ4787(del)
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<hr>Condition differences:<br>
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no differences
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*temperature:37C
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*medium:LB agar
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*medium:Puromycin dihydrochloride
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ECO:0007005
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high throughput direct assay evidence used in manual assertion
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PMID:21185072
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towards some (CHEBI:8642) Puromycin dihydrochloride
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*chemical concentration=  5 ug/ml
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*normalized fitness= -4.5
  
 
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Revision as of 20:32, 22 October 2019

Strain Summary

Strain Name

Escherichia coli K-12 JW0052

Synonyms

JW0052-1

Taxon Information
  • Pangenome: Escherichia coli
  • Subspecies and/or strain: K-12
  • NCBI Taxonomy ID: 83333
Genotype

DE(araD-araB)567 DE(rhaD-rhaB)568 F- Lambda- hsdR514 lacZ4787(del)(::rrnB-3) rph-1 surA765(del)::FRT-kan-FRT

Strain Reference
Strain availability

Coli Genetic Stock Center

Ancestry

parent:OMP ST:800 ! Escherichia coli K-12 BW25113

Annotated phenotypes


edit table


Notes

Phenotype Annotation

Annotation ID Qualifier OMP ID OMP term name Relative phenotype information Experimental condition ECO ID ECO term name Reference Annotation Extension Notes

OMP_AN:27863

OMP:0007249

decreased resistance to a chemical

Relative to: OMP_AN:27575

Genotype differences:

+lacZ4787(del)( +surA765(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Acriflavine (acriflavinium chloride)
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:74728) Acriflavine (acriflavinium chloride)

  • chemical concentration= 2 ug/ml
  • normalized fitness= -5.56

OMP_AN:27864

OMP:0007927

decreased anaerobic population growth

Relative to: OMP_AN:27618

Genotype differences:

+lacZ4787(del)( +surA765(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Anaerobic
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

  • normalized fitness= -4.8

OMP_AN:27865

OMP:0006036

decreased resistance to macrolide

Relative to: OMP_AN:27518

Genotype differences:

+lacZ4787(del)( +surA765(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Azithromycin
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:2955) Azithromycin

  • chemical concentration= 1 ug/ml
  • normalized fitness= -6.5

OMP_AN:27866

OMP:0007173

decreased resistance to host defense peptides

Relative to: OMP_AN:27562

Genotype differences:

+lacZ4787(del)( +surA765(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Cecropin B
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CID:16130488) Cecropin B

  • chemical concentration= 0.1 ug/ml
  • normalized fitness= -4.88

OMP_AN:27867

OMP:0006037

increased resistance to macrolide

Relative to: OMP_AN:27519

Genotype differences:

+lacZ4787(del)( +surA765(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Clarithromycin
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:3732) Clarithromycin

  • chemical concentration= 0.1 ug/ml
  • normalized fitness= 3.92

OMP_AN:27868

OMP:0007249

decreased resistance to a chemical

Relative to: OMP_AN:27510

Genotype differences:

+lacZ4787(del)( +surA765(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Mitomycin C
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:27504) Mitomycin C

  • chemical concentration= 0.1 ug/ml
  • normalized fitness= -8.77

OMP_AN:27869

OMP:0007879

decreased resistance to an antimicrobial agent

Relative to: OMP_AN:27553

Genotype differences:

+lacZ4787(del)( +surA765(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Puromycin dihydrochloride
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:8642) Puromycin dihydrochloride

  • chemical concentration= 5 ug/ml
  • normalized fitness= -4.5
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Additional Information

Links

References

See Help:References for how to manage references in OMPwiki.

  1. ↑ Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed OMPwiki page
  2. ↑ 2.0 2.1 2.2 2.3 2.4 2.5 2.6 Nichols, RJ et al. (2011) Phenotypic landscape of a bacterial cell. Cell 144 143-56 PubMed OMPwiki page
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