Difference between revisions of "OMP ST:71030 ! Escherichia coli K-12 BW25113 fabZ(F101Y)-kan"

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!align=left align='left' bgcolor='#CCCCFF' |Synonyms
 
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ECK0179-B-FABZ(F101Y)-KAN (Nichols et al. 2011. Supplemental Table S2)
 
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!align=left align='left' bgcolor='#CCCCFF' |Taxon Information
 
!align=left align='left' bgcolor='#CCCCFF' |Taxon Information
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!align=left align='left' bgcolor='#CCCCFF' |Ancestry
 
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parent: [[OMP ST:800 ! Escherichia coli K-12 BW25113]]
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parent:[[OMP ST:800 ! Escherichia coli K-12 BW25113]]
 
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!align=left align='left' bgcolor='#CCCCFF' |Annotated phenotypes
 
!align=left align='left' bgcolor='#CCCCFF' |Annotated phenotypes
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*chemical concentration= pH 4  
 
*chemical concentration= pH 4  
 
*normalized fitness= -3.81  
 
*normalized fitness= -3.81  
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OMP_AN:37252
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OMP:0006058
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increased resistance to aminoglycoside
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Relative to: OMP_AN:27527
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<hr>Genotype differences:<br>
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+lacZ4787(del)( +fabZ(F101Y)-kan  -lacZ4787(del)
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<hr>Condition differences:<br>
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no differences
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*temperature:37C
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*medium:LB agar
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*medium:Spectinomycin dihydrochloride pentahydrate
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ECO:0007005
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high throughput direct assay evidence used in manual assertion
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PMID:21185072
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towards some (CHEBI:9217) Spectinomycin dihydrochloride pentahydrate
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*chemical concentration= 4 ug/ml
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*normalized fitness= 3.72
  
 
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Latest revision as of 17:47, 8 July 2020

Strain Summary

Strain Name

Escherichia coli K-12 BW25113 fabZ(F101Y)-kan

Synonyms

ECK0179-B-FABZ(F101Y)-KAN (Nichols et al. 2011. Supplemental Table S2)

Taxon Information
  • Pangenome: Escherichia coli
  • Subspecies and/or strain: K-12
  • NCBI Taxonomy ID: 83333
Genotype

DE(araD-araB)567 DE(rhaD-rhaB)568 F- Lambda- hsdR514 lacZ4787(del)(::rrnB-3) rph-1 fabZ(F101Y)-kan

Strain Reference

PMID:21185072[1]

Strain availability
Ancestry

parent:OMP ST:800 ! Escherichia coli K-12 BW25113

Annotated phenotypes



Notes

Phenotype Annotation

Annotation ID Qualifier OMP ID OMP term name Relative phenotype information Experimental condition ECO ID ECO term name Reference Annotation Extension Notes

OMP_AN:37240

OMP:0006057

decreased resistance to aminoglycoside

Relative to: OMP_AN:27525


Genotype differences:

+lacZ4787(del)( +fabZ(F101Y)-kan -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Amikacin sulfate salt (disulfate)
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[1]

towards some (CHEBI:2638) Amikacin sulfate salt (disulfate)

  • chemical concentration= 0.1 ug/ml
  • normalized fitness= -3.86

OMP_AN:37241

OMP:0006044

decreased resistance to beta-lactam

Relative to: OMP_AN:27531


Genotype differences:

+lacZ4787(del)( +fabZ(F101Y)-kan -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Ampicillin
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[1]

towards some (CHEBI:28971) Ampicillin

  • chemical concentration= 1 ug/ml
  • normalized fitness= -3.84

OMP_AN:37242

OMP:0007879

decreased resistance to an antimicrobial agent

Relative to: OMP_AN:27552


Genotype differences:

+lacZ4787(del)( +fabZ(F101Y)-kan -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Azidothymidine
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[1]

towards some (CHEBI:10110) Azidothymidine

  • chemical concentration= 1 ng/ml
  • normalized fitness= -3.52

OMP_AN:37243

OMP:0007249

decreased resistance to a chemical

Relative to: OMP_AN:27592


Genotype differences:

+lacZ4787(del)( +fabZ(F101Y)-kan -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Calcofluor White M2R (F3543 Fluorescent Brightener 28)
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[1]

towards some (CHEBI:50010) Calcofluor White M2R (F3543 Fluorescent Brightener 28)

  • chemical concentration= 50 ug/ml
  • normalized fitness= -4.38

OMP_AN:37244

OMP:0007249

decreased resistance to a chemical

Relative to: OMP_AN:27512


Genotype differences:

+lacZ4787(del)( +fabZ(F101Y)-kan -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Hydroxyurea
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[1]

towards some (CHEBI:44423) Hydroxyurea

  • chemical concentration= 10 mM
  • normalized fitness= -3.96

OMP_AN:37245

OMP:0007886

decreased resistance to a metal

Relative to: OMP_AN:27580


Genotype differences:

+lacZ4787(del)( +fabZ(F101Y)-kan -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Nickel stress-Ni(II)Cl2 hexahydrate
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[1]

towards some (CHEBI:53542) Nickel stress-Ni(II)Cl2 hexahydrate

  • chemical concentration= 1 mM (LB)
  • normalized fitness= -3.4

OMP_AN:37246

OMP:0007879

decreased resistance to an antimicrobial agent

Relative to: OMP_AN:27564


Genotype differences:

+lacZ4787(del)( +fabZ(F101Y)-kan -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Phleomycin
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[1]

towards some (CHEBI:75044) Phleomycin

  • chemical concentration= 0.2 ug/ml
  • normalized fitness= -4.11

OMP_AN:37247

OMP:0006045

increased resistance to beta-lactam compound

Relative to: OMP_AN:27536


Genotype differences:

+lacZ4787(del)( +fabZ(F101Y)-kan -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Ceftazidime hydrate
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[1]

towards some (CHEBI:3509) Ceftazidime hydrate

  • chemical concentration= 0.075 ug/ml
  • normalized fitness= 3.16

OMP_AN:37248

OMP:0007879

decreased resistance to an antimicrobial agent

Relative to: OMP_AN:27558


Genotype differences:

+lacZ4787(del)( +fabZ(F101Y)-kan -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:D-Cycloserine
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[1]

towards some (CHEBI:40009) D-Cycloserine

  • chemical concentration= 16 ug/ml
  • normalized fitness= -3.69

OMP_AN:37249

OMP:0007520

increased population growth in high osmolarity

Relative to: OMP_AN:27595


Genotype differences:

+lacZ4787(del)( +fabZ(F101Y)-kan -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:NaCl
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[1]

towards some (CHEBI:26710) NaCl

  • chemical concentration= 600 mM
  • normalized fitness= 4

OMP_AN:37250

OMP:0007879

decreased resistance to an antimicrobial agent

Relative to: OMP_AN:27504


Genotype differences:

+lacZ4787(del)( +fabZ(F101Y)-kan -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Novobiocin sodium salt
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[1]

towards some (CHEBI:31924) Novobiocin sodium salt

  • chemical concentration= 8 ug/ml
  • normalized fitness= -8.42

OMP_AN:37251

OMP:0007008

decreased population growth at acidic pH

Relative to: OMP_AN:27622


Genotype differences:

+lacZ4787(del)( +fabZ(F101Y)-kan -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:MES-HOMOPIPES
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[1]

towards some (**SID:329817955 SID:57651422) MES-HOMOPIPES

  • chemical concentration= pH 4
  • normalized fitness= -3.81

OMP_AN:37252

OMP:0006058

increased resistance to aminoglycoside

Relative to: OMP_AN:27527


Genotype differences:

+lacZ4787(del)( +fabZ(F101Y)-kan -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Spectinomycin dihydrochloride pentahydrate
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[1]

towards some (CHEBI:9217) Spectinomycin dihydrochloride pentahydrate

  • chemical concentration= 4 ug/ml
  • normalized fitness= 3.72


Additional Information

Links

References

See Help:References for how to manage references in OMPwiki.

  1. 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 1.12 1.13 Nichols, RJ et al. (2011) Phenotypic landscape of a bacterial cell. Cell 144 143-56 PubMed OMPwiki page