Difference between revisions of "OMP ST:6169 ! Escherichia coli K-12 JW3030"

From OMPwiki
(touch)
(touch)
 
(14 intermediate revisions by the same user not shown)
Line 553: Line 553:
 
*chemical concentration= 0.2%
 
*chemical concentration= 0.2%
 
*normalized fitness= -8.02  
 
*normalized fitness= -8.02  
 +
|-
 +
|
 +
OMP_AN:32925
 +
|
 +
 +
|
 +
OMP:0007075
 +
|
 +
decreased growth factor requirement
 +
|
 +
Relative to: OMP_AN:27603
 +
 +
<hr>Genotype differences:<br>
 +
+lacZ4787(del)( +folB750(del)  -lacZ4787(del)
 +
<hr>Condition differences:<br>
 +
no differences
 +
|
 +
 +
*temperature:37C
 +
*medium:M9
 +
*medium:D-Glucose anhydrous
 +
|
 +
ECO:0007005
 +
|
 +
high throughput direct assay evidence used in manual assertion
 +
|
 +
PMID:21185072
 +
|
 +
towards some (CHEBI:42758) D-Glucose anhydrous
 +
|
 +
 +
*chemical concentration= 0.2%
 +
*normalized fitness= -3.89
 +
|-
 +
|
 +
OMP_AN:32926
 +
|
 +
 +
|
 +
OMP:0007070
 +
|
 +
decreased organic carbon source utilization
 +
|
 +
Relative to: OMP_AN:27613
 +
 +
<hr>Genotype differences:<br>
 +
+lacZ4787(del)( +folB750(del)  -lacZ4787(del)
 +
<hr>Condition differences:<br>
 +
no differences
 +
|
 +
 +
*temperature:37C
 +
*medium:M9
 +
*medium:D-Glucose anhydrous
 +
|
 +
ECO:0007005
 +
|
 +
high throughput direct assay evidence used in manual assertion
 +
|
 +
PMID:21185072
 +
|
 +
towards some (CHEBI:42758) D-Glucose anhydrous
 +
|
 +
 +
*chemical concentration= 0.2%
 +
*normalized fitness= -3.89
 +
|-
 +
|
 +
OMP_AN:32927
 +
|
 +
 +
|
 +
OMP:0007075
 +
|
 +
decreased growth factor requirement
 +
|
 +
Relative to: OMP_AN:27604
 +
 +
<hr>Genotype differences:<br>
 +
+lacZ4787(del)( +folB750(del)  -lacZ4787(del)
 +
<hr>Condition differences:<br>
 +
no differences
 +
|
 +
 +
*temperature:37C
 +
*medium:M9
 +
*medium:Glycerol
 +
|
 +
ECO:0007005
 +
|
 +
high throughput direct assay evidence used in manual assertion
 +
|
 +
PMID:21185072
 +
|
 +
towards some (CHEBI:17754) Glycerol
 +
|
 +
 +
*chemical concentration= 0.4%
 +
*normalized fitness= -6.58
 +
|-
 +
|
 +
OMP_AN:32928
 +
|
 +
 +
|
 +
OMP:0007070
 +
|
 +
decreased organic carbon source utilization
 +
|
 +
Relative to: OMP_AN:27614
 +
 +
<hr>Genotype differences:<br>
 +
+lacZ4787(del)( +folB750(del)  -lacZ4787(del)
 +
<hr>Condition differences:<br>
 +
no differences
 +
|
 +
 +
*temperature:37C
 +
*medium:M9
 +
*medium:Glycerol
 +
|
 +
ECO:0007005
 +
|
 +
high throughput direct assay evidence used in manual assertion
 +
|
 +
PMID:21185072
 +
|
 +
towards some (CHEBI:17754) Glycerol
 +
|
 +
 +
*chemical concentration= 0.4%
 +
*normalized fitness= -6.58
 +
|-
 +
|
 +
OMP_AN:32929
 +
|
 +
 +
|
 +
OMP:0007879
 +
|
 +
decreased resistance to an antimicrobial agent
 +
|
 +
Relative to: OMP_AN:27545
 +
 +
<hr>Genotype differences:<br>
 +
+lacZ4787(del)( +folB750(del)  -lacZ4787(del)
 +
<hr>Condition differences:<br>
 +
no differences
 +
|
 +
 +
*temperature:37C
 +
*medium:LB agar
 +
*medium:Isoniazid
 +
|
 +
ECO:0007005
 +
|
 +
high throughput direct assay evidence used in manual assertion
 +
|
 +
PMID:21185072
 +
|
 +
towards some (CHEBI:6030) Isoniazid
 +
|
 +
 +
*chemical concentration=  1 mM
 +
*normalized fitness= -5.74
 +
|-
 +
|
 +
OMP_AN:32930
 +
|
 +
 +
|
 +
OMP:0007075
 +
|
 +
decreased growth factor requirement
 +
|
 +
Relative to: OMP_AN:27600
 +
 +
<hr>Genotype differences:<br>
 +
+lacZ4787(del)( +folB750(del)  -lacZ4787(del)
 +
<hr>Condition differences:<br>
 +
no differences
 +
|
 +
 +
*temperature:37C
 +
*medium:MOPS
 +
*medium:NH4Cl
 +
|
 +
ECO:0007005
 +
|
 +
high throughput direct assay evidence used in manual assertion
 +
|
 +
PMID:21185072
 +
|
 +
towards some (CHEBI:31206) NH4Cl
 +
|
 +
 +
*chemical concentration= 9.5mm
 +
*normalized fitness= -8.98
 +
|-
 +
|
 +
OMP_AN:32931
 +
|
 +
 +
|
 +
OMP:0007032
 +
|
 +
decreased utilization of inorganic nitrogen source
 +
|
 +
Relative to: OMP_AN:27610
 +
 +
<hr>Genotype differences:<br>
 +
+lacZ4787(del)( +folB750(del)  -lacZ4787(del)
 +
<hr>Condition differences:<br>
 +
no differences
 +
|
 +
 +
*temperature:37C
 +
*medium:MOPS
 +
*medium:NH4Cl
 +
|
 +
ECO:0007005
 +
|
 +
high throughput direct assay evidence used in manual assertion
 +
|
 +
PMID:21185072
 +
|
 +
towards some (CHEBI:31206) NH4Cl
 +
|
 +
 +
*chemical concentration= 9.5mm
 +
*normalized fitness= -8.98
 +
|-
 +
|
 +
OMP_AN:32932
 +
|
 +
 +
|
 +
OMP:0007886
 +
|
 +
decreased resistance to a metal
 +
|
 +
Relative to: OMP_AN:27580
 +
 +
<hr>Genotype differences:<br>
 +
+lacZ4787(del)( +folB750(del)  -lacZ4787(del)
 +
<hr>Condition differences:<br>
 +
no differences
 +
|
 +
 +
*temperature:37C
 +
*medium:LB agar
 +
*medium:Nickel stress-Ni(II)Cl2 hexahydrate
 +
|
 +
ECO:0007005
 +
|
 +
high throughput direct assay evidence used in manual assertion
 +
|
 +
PMID:21185072
 +
|
 +
towards some (CHEBI:53542) Nickel stress-Ni(II)Cl2 hexahydrate
 +
|
 +
 +
*chemical concentration= 0.1 mM (LB)
 +
*normalized fitness= -4.05
 +
|-
 +
|
 +
OMP_AN:32933
 +
|
 +
 +
|
 +
OMP:0007075
 +
|
 +
decreased growth factor requirement
 +
|
 +
Relative to: OMP_AN:27607
 +
 +
<hr>Genotype differences:<br>
 +
+lacZ4787(del)( +folB750(del)  -lacZ4787(del)
 +
<hr>Condition differences:<br>
 +
no differences
 +
|
 +
 +
*temperature:37C
 +
*medium:M9
 +
*medium:Succinic acid
 +
|
 +
ECO:0007005
 +
|
 +
high throughput direct assay evidence used in manual assertion
 +
|
 +
PMID:21185072
 +
|
 +
towards some (CHEBI:15741) Succinic acid
 +
|
 +
 +
*chemical concentration= 0.3%
 +
*normalized fitness= -8.03
 +
|-
 +
|
 +
OMP_AN:32934
 +
|
 +
 +
|
 +
OMP:0007070
 +
|
 +
decreased organic carbon source utilization
 +
|
 +
Relative to: OMP_AN:27617
 +
 +
<hr>Genotype differences:<br>
 +
+lacZ4787(del)( +folB750(del)  -lacZ4787(del)
 +
<hr>Condition differences:<br>
 +
no differences
 +
|
 +
 +
*temperature:37C
 +
*medium:M9
 +
*medium:Succinic acid
 +
|
 +
ECO:0007005
 +
|
 +
high throughput direct assay evidence used in manual assertion
 +
|
 +
PMID:21185072
 +
|
 +
towards some (CHEBI:15741) Succinic acid
 +
|
 +
 +
*chemical concentration= 0.3%
 +
*normalized fitness= -8.03
 +
|-
 +
|
 +
OMP_AN:32935
 +
|
 +
 +
|
 +
OMP:0007249
 +
|
 +
decreased resistance to a chemical
 +
|
 +
Relative to: OMP_AN:27568
 +
 +
<hr>Genotype differences:<br>
 +
+lacZ4787(del)( +folB750(del)  -lacZ4787(del)
 +
<hr>Condition differences:<br>
 +
no differences
 +
|
 +
 +
*temperature:37C
 +
*medium:LB agar
 +
*medium:(±)-Verapamil hydrochloride
 +
|
 +
ECO:0007005
 +
|
 +
high throughput direct assay evidence used in manual assertion
 +
|
 +
PMID:21185072
 +
|
 +
towards some (CHEBI:53188) (±)-Verapamil hydrochloride
 +
|
 +
 +
*chemical concentration=  0.5 mM
 +
*normalized fitness= -3.78
 +
|-
 +
|
 +
OMP_AN:32936
 +
|
 +
 +
|
 +
OMP:0007075
 +
|
 +
decreased growth factor requirement
 +
|
 +
Relative to: OMP_AN:27598
 +
 +
<hr>Genotype differences:<br>
 +
+lacZ4787(del)( +folB750(del)  -lacZ4787(del)
 +
<hr>Condition differences:<br>
 +
no differences
 +
|
 +
 +
*temperature:37C
 +
*medium:MOPS
 +
*medium:Iron excess-FeSO4 heptahydride
 +
|
 +
ECO:0007005
 +
|
 +
high throughput direct assay evidence used in manual assertion
 +
|
 +
PMID:21185072
 +
|
 +
towards some (CHEBI:75836) Iron excess-FeSO4 heptahydride
 +
|
 +
 +
*chemical concentration= 1mM (MOPS; normal 100uM)
 +
*normalized fitness= -3.38
 +
|-
 +
|
 +
OMP_AN:32937
 +
|
 +
 +
|
 +
OMP:0007886
 +
|
 +
decreased resistance to a metal
 +
|
 +
Relative to: OMP_AN:27608
 +
 +
<hr>Genotype differences:<br>
 +
+lacZ4787(del)( +folB750(del)  -lacZ4787(del)
 +
<hr>Condition differences:<br>
 +
no differences
 +
|
 +
 +
*temperature:37C
 +
*medium:MOPS
 +
*medium:Iron excess-FeSO4 heptahydride
 +
|
 +
ECO:0007005
 +
|
 +
high throughput direct assay evidence used in manual assertion
 +
|
 +
PMID:21185072
 +
|
 +
towards some (CHEBI:75836) Iron excess-FeSO4 heptahydride
 +
|
 +
 +
*chemical concentration= 1mM (MOPS; normal 100uM)
 +
*normalized fitness= -3.38
 +
|-
 +
|
 +
OMP_AN:32938
 +
|
 +
 +
|
 +
OMP:0007075
 +
|
 +
decreased growth factor requirement
 +
|
 +
Relative to: OMP_AN:27599
 +
 +
<hr>Genotype differences:<br>
 +
+lacZ4787(del)( +folB750(del)  -lacZ4787(del)
 +
<hr>Condition differences:<br>
 +
no differences
 +
|
 +
 +
*temperature:37C
 +
*medium:MOPS
 +
*medium:Iron starvation-FeSO4·7H2O
 +
|
 +
ECO:0007005
 +
|
 +
high throughput direct assay evidence used in manual assertion
 +
|
 +
PMID:21185072
 +
|
 +
towards some (CHEBI:75836) Iron starvation-FeSO4·7H2O
 +
|
 +
 +
*chemical concentration= 2uM (MOPS; normal 100uM)
 +
*normalized fitness= -7.46
 +
|-
 +
|
 +
OMP_AN:32939
 +
|
 +
 +
|
 +
OMP:0007937
 +
|
 +
decreased resistance to nutrient limitation
 +
|
 +
Relative to: OMP_AN:27609
 +
 +
<hr>Genotype differences:<br>
 +
+lacZ4787(del)( +folB750(del)  -lacZ4787(del)
 +
<hr>Condition differences:<br>
 +
no differences
 +
|
 +
 +
*temperature:37C
 +
*medium:MOPS
 +
*medium:Iron starvation-FeSO4·7H2O
 +
|
 +
ECO:0007005
 +
|
 +
high throughput direct assay evidence used in manual assertion
 +
|
 +
PMID:21185072
 +
|
 +
towards some (CHEBI:75836) Iron starvation-FeSO4·7H2O
 +
|
 +
 +
*chemical concentration= 2uM (MOPS; normal 100uM)
 +
*normalized fitness= -7.46
  
 
|- class="tableEdit_footer"  
 
|- class="tableEdit_footer"  

Latest revision as of 01:12, 23 October 2019

Strain Summary

Strain Name

Escherichia coli K-12 JW3030

Synonyms

JW3030-2

Taxon Information
  • Pangenome: Escherichia coli
  • Subspecies and/or strain: K-12
  • NCBI Taxonomy ID: 83333
Genotype

DE(araD-araB)567 DE(rhaD-rhaB)568 F- Lambda- hsdR514 lacZ4787(del)(::rrnB-3) rph-1 folB750(del)::kan

Strain Reference

Coli Genetic Stock Center

Strain availability

Coli Genetic Stock Center

Ancestry

parent:OMP ST:800 ! Escherichia coli K-12 BW25113

Annotated phenotypes


edit table


Notes

Phenotype Annotation

Annotation ID Qualifier OMP ID OMP term name Relative phenotype information Experimental condition ECO ID ECO term name Reference Annotation Extension Notes

OMP_AN:1281

OMP:0007092

decreased cell motility by swarming

Relative to: OMP_AN:881

Genotype differences:

+DE(araD-araB)567 +DE(rhaD-rhaB)568 +hsdR514 +lacZ4787(del)( +folB750(del) -IN(rrnD-rrnE)1

Condition differences:

no differences

  • temperature:33C
  • medium:swarming motility medium
 ECO:0005803

motility assay evidence

PMID:17122336[1]

OMP_AN:1282

OMP:0007093

decreased cell motility by swimming

Relative to: OMP_AN:882

Genotype differences:

+DE(araD-araB)567 +DE(rhaD-rhaB)568 +hsdR514 +lacZ4787(del)( +folB750(del) -IN(rrnD-rrnE)1

Condition differences:

no differences

  • temperature:33C
  • medium:swimming motility medium
 ECO:0005803

motility assay evidence

PMID:17122336[1]

OMP_AN:32912

OMP:0007879

decreased resistance to an antimicrobial agent

Relative to: OMP_AN:27557

Genotype differences:

+lacZ4787(del)( +folB750(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:A22
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CID:2830968) A22

  • chemical concentration= 15 ug/ml
  • normalized fitness= -7.18

OMP_AN:32913

OMP:0007879

decreased resistance to an antimicrobial agent

Relative to: OMP_AN:27559

Genotype differences:

+lacZ4787(del)( +folB750(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Actinomycin D
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:27666 CID:457193) Actinomycin D

  • chemical concentration= 15 ug/ml
  • normalized fitness= -3.7

OMP_AN:32914

OMP:0007879

decreased resistance to an antimicrobial agent

Relative to: OMP_AN:27552

Genotype differences:

+lacZ4787(del)( +folB750(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Azidothymidine
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:10110) Azidothymidine

  • chemical concentration= 2.5 ng/ml
  • normalized fitness= -3.68

OMP_AN:32915

OMP:0007249

decreased resistance to a chemical

Relative to: OMP_AN:27514

Genotype differences:

+lacZ4787(del)( +folB750(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Chlorpromazine hydrochloride
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:3649) Chlorpromazine hydrochloride

  • chemical concentration= 6 uM
  • normalized fitness= -5.24

OMP_AN:32916

OMP:0007903

decreased resistance to bile acids

Relative to: OMP_AN:27586

Genotype differences:

+lacZ4787(del)( +folB750(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Deoxycholate sodium
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:9177) Deoxycholate sodium

  • chemical concentration= 2 %
  • normalized fitness= -7.18

OMP_AN:32917

OMP:0007249

decreased resistance to a chemical

Relative to: OMP_AN:27501

Genotype differences:

+lacZ4787(del)( +folB750(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Dibucaine
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:247956) Dibucaine

  • chemical concentration= 0.8 mM
  • normalized fitness= -3.41

OMP_AN:32918

OMP:0006036

decreased resistance to macrolide

Relative to: OMP_AN:27520

Genotype differences:

+lacZ4787(del)( +folB750(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Erythromycin
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:42355) Erythromycin

  • chemical concentration= 0.1 ug/ml
  • normalized fitness= -3.43

OMP_AN:32919

OMP:0007879

decreased resistance to an antimicrobial agent

Relative to: OMP_AN:27555

Genotype differences:

+lacZ4787(del)( +folB750(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Fosfomycin disodium salt
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CID:6093162) Fosfomycin disodium salt

  • chemical concentration= 1 ug/ml
  • normalized fitness= -3.21

OMP_AN:32920

OMP:0007075

decreased growth factor requirement

Relative to: OMP_AN:27606

Genotype differences:

+lacZ4787(del)( +folB750(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:M9
  • medium:N-Acetyl-D-glucosamine
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:506227) N-Acetyl-D-glucosamine

  • chemical concentration= 0.15%
  • normalized fitness= -4.21

OMP_AN:32921

OMP:0007070

decreased organic carbon source utilization

Relative to: OMP_AN:27616

Genotype differences:

+lacZ4787(del)( +folB750(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:M9
  • medium:N-Acetyl-D-glucosamine
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:506227) N-Acetyl-D-glucosamine

  • chemical concentration= 0.15%
  • normalized fitness= -4.21

OMP_AN:32922

OMP:0007879

decreased resistance to an antimicrobial agent

Relative to: OMP_AN:27590

Genotype differences:

+lacZ4787(del)( +folB750(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Fosfomycin (CID:6093162) +Glucose-6-phosphate (CHEBI:4170)
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CID:6093162 + CHEBI:4170) Fosfomycin (CID:6093162) +Glucose-6-phosphate (CHEBI:4170)

  • chemical concentration= 0.2 ug/ml (in 50 mg/ml G6P)
  • normalized fitness= -4.48

OMP_AN:32923

OMP:0007075

decreased growth factor requirement

Relative to: OMP_AN:27602

Genotype differences:

+lacZ4787(del)( +folB750(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:M9
  • medium:D-(+) Glucosamine hydrochloride
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CID:91431) D-(+) Glucosamine hydrochloride

  • chemical concentration= 0.2%
  • normalized fitness= -8.02

OMP_AN:32924

OMP:0007070

decreased organic carbon source utilization

Relative to: OMP_AN:27612

Genotype differences:

+lacZ4787(del)( +folB750(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:M9
  • medium:D-(+) Glucosamine hydrochloride
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CID:91431) D-(+) Glucosamine hydrochloride

  • chemical concentration= 0.2%
  • normalized fitness= -8.02

OMP_AN:32925

OMP:0007075

decreased growth factor requirement

Relative to: OMP_AN:27603

Genotype differences:

+lacZ4787(del)( +folB750(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:M9
  • medium:D-Glucose anhydrous
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:42758) D-Glucose anhydrous

  • chemical concentration= 0.2%
  • normalized fitness= -3.89

OMP_AN:32926

OMP:0007070

decreased organic carbon source utilization

Relative to: OMP_AN:27613

Genotype differences:

+lacZ4787(del)( +folB750(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:M9
  • medium:D-Glucose anhydrous
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:42758) D-Glucose anhydrous

  • chemical concentration= 0.2%
  • normalized fitness= -3.89

OMP_AN:32927

OMP:0007075

decreased growth factor requirement

Relative to: OMP_AN:27604

Genotype differences:

+lacZ4787(del)( +folB750(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:M9
  • medium:Glycerol
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:17754) Glycerol

  • chemical concentration= 0.4%
  • normalized fitness= -6.58

OMP_AN:32928

OMP:0007070

decreased organic carbon source utilization

Relative to: OMP_AN:27614

Genotype differences:

+lacZ4787(del)( +folB750(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:M9
  • medium:Glycerol
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:17754) Glycerol

  • chemical concentration= 0.4%
  • normalized fitness= -6.58

OMP_AN:32929

OMP:0007879

decreased resistance to an antimicrobial agent

Relative to: OMP_AN:27545

Genotype differences:

+lacZ4787(del)( +folB750(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Isoniazid
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:6030) Isoniazid

  • chemical concentration= 1 mM
  • normalized fitness= -5.74

OMP_AN:32930

OMP:0007075

decreased growth factor requirement

Relative to: OMP_AN:27600

Genotype differences:

+lacZ4787(del)( +folB750(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:MOPS
  • medium:NH4Cl
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:31206) NH4Cl

  • chemical concentration= 9.5mm
  • normalized fitness= -8.98

OMP_AN:32931

OMP:0007032

decreased utilization of inorganic nitrogen source

Relative to: OMP_AN:27610

Genotype differences:

+lacZ4787(del)( +folB750(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:MOPS
  • medium:NH4Cl
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:31206) NH4Cl

  • chemical concentration= 9.5mm
  • normalized fitness= -8.98

OMP_AN:32932

OMP:0007886

decreased resistance to a metal

Relative to: OMP_AN:27580

Genotype differences:

+lacZ4787(del)( +folB750(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Nickel stress-Ni(II)Cl2 hexahydrate
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:53542) Nickel stress-Ni(II)Cl2 hexahydrate

  • chemical concentration= 0.1 mM (LB)
  • normalized fitness= -4.05

OMP_AN:32933

OMP:0007075

decreased growth factor requirement

Relative to: OMP_AN:27607

Genotype differences:

+lacZ4787(del)( +folB750(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:M9
  • medium:Succinic acid
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:15741) Succinic acid

  • chemical concentration= 0.3%
  • normalized fitness= -8.03

OMP_AN:32934

OMP:0007070

decreased organic carbon source utilization

Relative to: OMP_AN:27617

Genotype differences:

+lacZ4787(del)( +folB750(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:M9
  • medium:Succinic acid
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:15741) Succinic acid

  • chemical concentration= 0.3%
  • normalized fitness= -8.03

OMP_AN:32935

OMP:0007249

decreased resistance to a chemical

Relative to: OMP_AN:27568

Genotype differences:

+lacZ4787(del)( +folB750(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:(±)-Verapamil hydrochloride
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:53188) (±)-Verapamil hydrochloride

  • chemical concentration= 0.5 mM
  • normalized fitness= -3.78

OMP_AN:32936

OMP:0007075

decreased growth factor requirement

Relative to: OMP_AN:27598

Genotype differences:

+lacZ4787(del)( +folB750(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:MOPS
  • medium:Iron excess-FeSO4 heptahydride
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:75836) Iron excess-FeSO4 heptahydride

  • chemical concentration= 1mM (MOPS; normal 100uM)
  • normalized fitness= -3.38

OMP_AN:32937

OMP:0007886

decreased resistance to a metal

Relative to: OMP_AN:27608

Genotype differences:

+lacZ4787(del)( +folB750(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:MOPS
  • medium:Iron excess-FeSO4 heptahydride
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:75836) Iron excess-FeSO4 heptahydride

  • chemical concentration= 1mM (MOPS; normal 100uM)
  • normalized fitness= -3.38

OMP_AN:32938

OMP:0007075

decreased growth factor requirement

Relative to: OMP_AN:27599

Genotype differences:

+lacZ4787(del)( +folB750(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:MOPS
  • medium:Iron starvation-FeSO4·7H2O
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:75836) Iron starvation-FeSO4·7H2O

  • chemical concentration= 2uM (MOPS; normal 100uM)
  • normalized fitness= -7.46

OMP_AN:32939

OMP:0007937

decreased resistance to nutrient limitation

Relative to: OMP_AN:27609

Genotype differences:

+lacZ4787(del)( +folB750(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:MOPS
  • medium:Iron starvation-FeSO4·7H2O
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:75836) Iron starvation-FeSO4·7H2O

  • chemical concentration= 2uM (MOPS; normal 100uM)
  • normalized fitness= -7.46
edit table


Additional Information

Links

References

See Help:References for how to manage references in OMPwiki.

  1. ↑ 1.0 1.1 Inoue, T et al. (2007) Genome-wide screening of genes required for swarming motility in Escherichia coli K-12. J. Bacteriol. 189 950-7 PubMed OMPwiki page
  2. ↑ 2.00 2.01 2.02 2.03 2.04 2.05 2.06 2.07 2.08 2.09 2.10 2.11 2.12 2.13 2.14 2.15 2.16 2.17 2.18 2.19 2.20 2.21 2.22 2.23 2.24 2.25 2.26 2.27 Nichols, RJ et al. (2011) Phenotypic landscape of a bacterial cell. Cell 144 143-56 PubMed OMPwiki page
Retrieved from "https://microbialphenotypes.org/wiki/index.php?title=OMP_ST:6169_!_Escherichia_coli_K-12_JW3030&oldid=138432"