Difference between revisions of "OMP ST:1114 ! Escherichia coli K-12 JW0511"

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*chemical concentration= 9.5mm
 
*chemical concentration= 9.5mm
 
*normalized fitness= -15.42  
 
*normalized fitness= -15.42  
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OMP_AN:28613
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OMP:0007879
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decreased resistance to an antimicrobial agent
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Relative to: OMP_AN:27503
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<hr>Genotype differences:<br>
 +
+lacZ4787(del)( +purK735(del)  -lacZ4787(del)
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<hr>Condition differences:<br>
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no differences
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*temperature:37C
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*medium:LB agar
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*medium:Nitrofurantoin
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ECO:0007005
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high throughput direct assay evidence used in manual assertion
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PMID:21185072
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towards some (CHEBI:71415) Nitrofurantoin
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*chemical concentration=  1.5 ug/ml
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*normalized fitness= -3.45
  
 
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Revision as of 20:59, 22 October 2019

Strain Summary

Strain Name

Escherichia coli K-12 JW0511

Synonyms

JW0511-1

Taxon Information
  • Pangenome: Escherichia coli
  • Subspecies and/or strain: K-12
  • NCBI Taxonomy ID: 83333
Genotype

DE(araD-araB)567 DE(rhaD-rhaB)568 F- Lambda- hsdR514 lacZ4787(del)(::rrnB-3) rph-1 purK735(del)::kan

Strain Reference

Coli Genetic Stock Center

Strain availability

Coli Genetic Stock Center

Ancestry

parent:OMP ST:800 ! Escherichia coli K-12 BW25113

Annotated phenotypes


edit table


Notes

Phenotype Annotation

Annotation ID Qualifier OMP ID OMP term name Relative phenotype information Experimental condition ECO ID ECO term name Reference Annotation Extension Notes

OMP_AN:1029

OMP:0007092

decreased cell motility by swarming

Relative to: OMP_AN:881

Genotype differences:

+DE(araD-araB)567 +DE(rhaD-rhaB)568 +hsdR514 +lacZ4787(del)( +purK735(del) -IN(rrnD-rrnE)1

Condition differences:

no differences

  • temperature:33C
  • medium:swarming motility medium
 ECO:0005803

motility assay evidence

PMID:17122336[1]

OMP_AN:28595

OMP:0007879

decreased resistance to an antimicrobial agent

Relative to: OMP_AN:27559

Genotype differences:

+lacZ4787(del)( +purK735(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Actinomycin D
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:27666 CID:457193) Actinomycin D

  • chemical concentration= 15 ug/ml
  • normalized fitness= -3.72

OMP_AN:28596

OMP:0007075

decreased growth factor requirement

Relative to: OMP_AN:27601

Genotype differences:

+lacZ4787(del)( +purK735(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:M9
  • medium:Sodium Acetate anhydrous
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:32954) Sodium Acetate anhydrous

  • chemical concentration= 0.6%
  • normalized fitness= -11.98

OMP_AN:28597

OMP:0007070

decreased organic carbon source utilization

Relative to: OMP_AN:27611

Genotype differences:

+lacZ4787(del)( +purK735(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:M9
  • medium:Sodium Acetate anhydrous
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:32954) Sodium Acetate anhydrous

  • chemical concentration= 0.6%
  • normalized fitness= -11.98

OMP_AN:28598

OMP:0006044

decreased resistance to beta-lactam

Relative to: OMP_AN:27534

Genotype differences:

+lacZ4787(del)( +purK735(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Cefoxitin sodium hydrate
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:3501) Cefoxitin sodium hydrate

  • chemical concentration= 0.25 ug/ml
  • normalized fitness= -5.48

OMP_AN:28599

OMP:0007903

decreased resistance to bile acids

Relative to: OMP_AN:27586

Genotype differences:

+lacZ4787(del)( +purK735(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Deoxycholate sodium
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:9177) Deoxycholate sodium

  • chemical concentration= 2 %
  • normalized fitness= -3.49

OMP_AN:28600

OMP:0007075

decreased growth factor requirement

Relative to: OMP_AN:27606

Genotype differences:

+lacZ4787(del)( +purK735(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:M9
  • medium:N-Acetyl-D-glucosamine
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:506227) N-Acetyl-D-glucosamine

  • chemical concentration= 0.15%
  • normalized fitness= -12.37

OMP_AN:28601

OMP:0007070

decreased organic carbon source utilization

Relative to: OMP_AN:27616

Genotype differences:

+lacZ4787(del)( +purK735(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:M9
  • medium:N-Acetyl-D-glucosamine
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:506227) N-Acetyl-D-glucosamine

  • chemical concentration= 0.15%
  • normalized fitness= -12.37

OMP_AN:28602

OMP:0007075

decreased growth factor requirement

Relative to: OMP_AN:27602

Genotype differences:

+lacZ4787(del)( +purK735(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:M9
  • medium:D-(+) Glucosamine hydrochloride
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CID:91431) D-(+) Glucosamine hydrochloride

  • chemical concentration= 0.2%
  • normalized fitness= -17.39

OMP_AN:28603

OMP:0007070

decreased organic carbon source utilization

Relative to: OMP_AN:27612

Genotype differences:

+lacZ4787(del)( +purK735(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:M9
  • medium:D-(+) Glucosamine hydrochloride
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CID:91431) D-(+) Glucosamine hydrochloride

  • chemical concentration= 0.2%
  • normalized fitness= -17.39

OMP_AN:28604

OMP:0007075

decreased growth factor requirement

Relative to: OMP_AN:27603

Genotype differences:

+lacZ4787(del)( +purK735(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:M9
  • medium:D-Glucose anhydrous
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:42758) D-Glucose anhydrous

  • chemical concentration= 0.2%
  • normalized fitness= -10.88

OMP_AN:28605

OMP:0007070

decreased organic carbon source utilization

Relative to: OMP_AN:27613

Genotype differences:

+lacZ4787(del)( +purK735(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:M9
  • medium:D-Glucose anhydrous
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:42758) D-Glucose anhydrous

  • chemical concentration= 0.2%
  • normalized fitness= -10.88

OMP_AN:28606

OMP:0007075

decreased growth factor requirement

Relative to: OMP_AN:27604

Genotype differences:

+lacZ4787(del)( +purK735(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:M9
  • medium:Glycerol
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:17754) Glycerol

  • chemical concentration= 0.4%
  • normalized fitness= -13.21

OMP_AN:28607

OMP:0007070

decreased organic carbon source utilization

Relative to: OMP_AN:27614

Genotype differences:

+lacZ4787(del)( +purK735(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:M9
  • medium:Glycerol
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:17754) Glycerol

  • chemical concentration= 0.4%
  • normalized fitness= -13.21

OMP_AN:28608

OMP:0007075

decreased growth factor requirement

Relative to: OMP_AN:27605

Genotype differences:

+lacZ4787(del)( +purK735(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:M9
  • medium:D-(+) Maltose monohydrate
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CID:23615261) D-(+) Maltose monohydrate

  • chemical concentration= 0.1%
  • normalized fitness= -10.75

OMP_AN:28609

OMP:0007070

decreased organic carbon source utilization

Relative to: OMP_AN:27615

Genotype differences:

+lacZ4787(del)( +purK735(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:M9
  • medium:D-(+) Maltose monohydrate
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CID:23615261) D-(+) Maltose monohydrate

  • chemical concentration= 0.1%
  • normalized fitness= -10.75

OMP_AN:28610

OMP:0006044

decreased resistance to beta-lactam

Relative to: OMP_AN:27537

Genotype differences:

+lacZ4787(del)( +purK735(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Mecillinam
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:51208) Mecillinam

  • chemical concentration= 0.03 ug/ml
  • normalized fitness= -3.88

OMP_AN:28611

OMP:0007075

decreased growth factor requirement

Relative to: OMP_AN:27600

Genotype differences:

+lacZ4787(del)( +purK735(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:MOPS
  • medium:NH4Cl
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:31206) NH4Cl

  • chemical concentration= 9.5mm
  • normalized fitness= -15.42

OMP_AN:28612

OMP:0007032

decreased utilization of inorganic nitrogen source

Relative to: OMP_AN:27610

Genotype differences:

+lacZ4787(del)( +purK735(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:MOPS
  • medium:NH4Cl
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:31206) NH4Cl

  • chemical concentration= 9.5mm
  • normalized fitness= -15.42

OMP_AN:28613

OMP:0007879

decreased resistance to an antimicrobial agent

Relative to: OMP_AN:27503

Genotype differences:

+lacZ4787(del)( +purK735(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Nitrofurantoin
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:71415) Nitrofurantoin

  • chemical concentration= 1.5 ug/ml
  • normalized fitness= -3.45
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Additional Information

Links

References

See Help:References for how to manage references in OMPwiki.

  1. ↑ Inoue, T et al. (2007) Genome-wide screening of genes required for swarming motility in Escherichia coli K-12. J. Bacteriol. 189 950-7 PubMed OMPwiki page
  2. ↑ 2.00 2.01 2.02 2.03 2.04 2.05 2.06 2.07 2.08 2.09 2.10 2.11 2.12 2.13 2.14 2.15 2.16 2.17 2.18 Nichols, RJ et al. (2011) Phenotypic landscape of a bacterial cell. Cell 144 143-56 PubMed OMPwiki page
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