OMP ST:74597 ! Escherichia coli K-12 JW5856

From OMPwiki

Strain Summary

Strain Name

Escherichia coli K-12 JW5856

Synonyms

JW5856-2

Taxon Information
  • Pangenome: Escherichia coli
  • Subspecies and/or strain: K-12
  • NCBI Taxonomy ID: 83333
Genotype

DE(araD-araB)567 DE(rhaD-rhaB)568 F- Lambda- hsdR514 lacZ4787(del)(::rrnB-3) rph-1 trxA732(del)::FRT-kan-FRT

Strain Reference
Strain availability

Coli Genetic Stock Center

Ancestry

parent:OMP ST:800 ! Escherichia coli K-12 BW25113

Annotated phenotypes



Notes

Phenotype Annotation

Annotation ID Qualifier OMP ID OMP term name Relative phenotype information Experimental condition ECO ID ECO term name Reference Annotation Extension Notes

OMP_AN:36767

OMP:0007879

decreased resistance to an antimicrobial agent

Relative to: OMP_AN:27552


Genotype differences:

+lacZ4787(del)( +trxA732(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Azidothymidine
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:10110) Azidothymidine

  • chemical concentration= 2.5 ng/ml
  • normalized fitness= -4.14

OMP_AN:36768

OMP:0007879

decreased resistance to an antimicrobial agent

Relative to: OMP_AN:27556


Genotype differences:

+lacZ4787(del)( +trxA732(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Bicyclomycin
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:60584) Bicyclomycin

  • chemical concentration= 10 ug/ml
  • normalized fitness= -3.87

OMP_AN:36769

OMP:0007879

decreased resistance to an antimicrobial agent

Relative to: OMP_AN:27549


Genotype differences:

+lacZ4787(del)( +trxA732(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:CHIR-090
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:134107 CID:11546620) CHIR-090

  • chemical concentration= 0.05 ug/ml
  • normalized fitness= -3.16

OMP_AN:36770

OMP:0007075

decreased growth factor requirement

Relative to: OMP_AN:27606


Genotype differences:

+lacZ4787(del)( +trxA732(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:M9
  • medium:N-Acetyl-D-glucosamine
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:506227) N-Acetyl-D-glucosamine

  • chemical concentration= 0.15%
  • normalized fitness= -3.4

OMP_AN:36771

OMP:0007070

decreased organic carbon source utilization

Relative to: OMP_AN:27616


Genotype differences:

+lacZ4787(del)( +trxA732(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:M9
  • medium:N-Acetyl-D-glucosamine
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:506227) N-Acetyl-D-glucosamine

  • chemical concentration= 0.15%
  • normalized fitness= -3.4

OMP_AN:36772

OMP:0007075

decreased growth factor requirement

Relative to: OMP_AN:27603


Genotype differences:

+lacZ4787(del)( +trxA732(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:M9
  • medium:D-Glucose anhydrous
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:42758) D-Glucose anhydrous

  • chemical concentration= 0.2%
  • normalized fitness= -4.71

OMP_AN:36773

OMP:0007070

decreased organic carbon source utilization

Relative to: OMP_AN:27613


Genotype differences:

+lacZ4787(del)( +trxA732(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:M9
  • medium:D-Glucose anhydrous
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:42758) D-Glucose anhydrous

  • chemical concentration= 0.2%
  • normalized fitness= -4.71

OMP_AN:36774

OMP:0007075

decreased growth factor requirement

Relative to: OMP_AN:27604


Genotype differences:

+lacZ4787(del)( +trxA732(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:M9
  • medium:Glycerol
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:17754) Glycerol

  • chemical concentration= 0.4%
  • normalized fitness= -5.7

OMP_AN:36775

OMP:0007070

decreased organic carbon source utilization

Relative to: OMP_AN:27614


Genotype differences:

+lacZ4787(del)( +trxA732(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:M9
  • medium:Glycerol
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:17754) Glycerol

  • chemical concentration= 0.4%
  • normalized fitness= -5.7

OMP_AN:36776

OMP:0007075

decreased growth factor requirement

Relative to: OMP_AN:27605


Genotype differences:

+lacZ4787(del)( +trxA732(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:M9
  • medium:D-(+) Maltose monohydrate
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CID:23615261) D-(+) Maltose monohydrate

  • chemical concentration= 0.1%
  • normalized fitness= -5.34

OMP_AN:36777

OMP:0007070

decreased organic carbon source utilization

Relative to: OMP_AN:27615


Genotype differences:

+lacZ4787(del)( +trxA732(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:M9
  • medium:D-(+) Maltose monohydrate
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CID:23615261) D-(+) Maltose monohydrate

  • chemical concentration= 0.1%
  • normalized fitness= -5.34

OMP_AN:36778

OMP:0007075

decreased growth factor requirement

Relative to: OMP_AN:27600


Genotype differences:

+lacZ4787(del)( +trxA732(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:MOPS
  • medium:NH4Cl
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:31206) NH4Cl

  • chemical concentration= 9.5mm
  • normalized fitness= -5.23

OMP_AN:36779

OMP:0007032

decreased utilization of inorganic nitrogen source

Relative to: OMP_AN:27610


Genotype differences:

+lacZ4787(del)( +trxA732(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:MOPS
  • medium:NH4Cl
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:31206) NH4Cl

  • chemical concentration= 9.5mm
  • normalized fitness= -5.23

OMP_AN:36780

OMP:0007878

increased resistance to an antimicrobial agent

Relative to: OMP_AN:27558


Genotype differences:

+lacZ4787(del)( +trxA732(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:D-Cycloserine
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:40009) D-Cycloserine

  • chemical concentration= 16 ug/ml
  • normalized fitness= 3.71

OMP_AN:36781

OMP:0007075

decreased growth factor requirement

Relative to: OMP_AN:27598


Genotype differences:

+lacZ4787(del)( +trxA732(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:MOPS
  • medium:Iron excess-FeSO4 heptahydride
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:75836) Iron excess-FeSO4 heptahydride

  • chemical concentration= 1mM (MOPS; normal 100uM)
  • normalized fitness= -3.98

OMP_AN:36782

OMP:0007886

decreased resistance to a metal

Relative to: OMP_AN:27608


Genotype differences:

+lacZ4787(del)( +trxA732(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:MOPS
  • medium:Iron excess-FeSO4 heptahydride
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:75836) Iron excess-FeSO4 heptahydride

  • chemical concentration= 1mM (MOPS; normal 100uM)
  • normalized fitness= -3.98

OMP_AN:36783

OMP:0007075

decreased growth factor requirement

Relative to: OMP_AN:27599


Genotype differences:

+lacZ4787(del)( +trxA732(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:MOPS
  • medium:Iron starvation-FeSO4·7H2O
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:75836) Iron starvation-FeSO4·7H2O

  • chemical concentration= 2uM (MOPS; normal 100uM)
  • normalized fitness= -4.5

OMP_AN:36784

OMP:0007937

decreased resistance to nutrient limitation

Relative to: OMP_AN:27609


Genotype differences:

+lacZ4787(del)( +trxA732(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:MOPS
  • medium:Iron starvation-FeSO4·7H2O
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:75836) Iron starvation-FeSO4·7H2O

  • chemical concentration= 2uM (MOPS; normal 100uM)
  • normalized fitness= -4.5

OMP_AN:36785

OMP:0005098

decreased resistance to UV radiation 

Relative to: OMP_AN:27619


Genotype differences:

+lacZ4787(del)( +trxA732(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:UV
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

  • chemical concentration= 18 sec
  • normalized fitness= -3.63


Additional Information

Links

References

See Help:References for how to manage references in OMPwiki.

  1. Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed OMPwiki page
  2. 2.00 2.01 2.02 2.03 2.04 2.05 2.06 2.07 2.08 2.09 2.10 2.11 2.12 2.13 2.14 2.15 2.16 2.17 2.18 Nichols, RJ et al. (2011) Phenotypic landscape of a bacterial cell. Cell 144 143-56 PubMed OMPwiki page