OMP ST:74195 ! Escherichia coli K-12 JW5260

From OMPwiki

Strain Summary

Strain Name

Escherichia coli K-12 JW5260

Synonyms

JW5260-1

Taxon Information
  • Pangenome: Escherichia coli
  • Subspecies and/or strain: K-12
  • NCBI Taxonomy ID: 83333
Genotype

DE(araD-araB)567 DE(rhaD-rhaB)568 F- Lambda- hsdR514 lacZ4787(del)(::rrnB-3) rph-1 ynfF736(del)::FRT-kan-FRT

Strain Reference
Strain availability

Coli Genetic Stock Center

Ancestry

parent:OMP ST:800 ! Escherichia coli K-12 BW25113

Annotated phenotypes



Notes

Phenotype Annotation

Showing 1 to 10 of 11 entries
Annotation IDQualifierOMP IDOMP term nameRelative phenotype informationExperimental conditionECO IDECO term nameReferenceAnnotation ExtensionNotes

OMP_AN:35922

OMP:0007074

increased growth factor requirement

Relative to: OMP_AN:27601


Genotype differences:

+lacZ4787(del)( +ynfF736(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:M9
  • medium:Sodium Acetate anhydrous
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:32954) Sodium Acetate anhydrous

  • chemical concentration= 0.6%
  • normalized fitness= 4.88

OMP_AN:35923

OMP:0007071

increased organic carbon source utilization

Relative to: OMP_AN:27611


Genotype differences:

+lacZ4787(del)( +ynfF736(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:M9
  • medium:Sodium Acetate anhydrous
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:32954) Sodium Acetate anhydrous

  • chemical concentration= 0.6%
  • normalized fitness= 4.88

OMP_AN:35924

OMP:0006037

increased resistance to macrolide

Relative to: OMP_AN:27518


Genotype differences:

+lacZ4787(del)( +ynfF736(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Azithromycin
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:2955) Azithromycin

  • chemical concentration= 1 ug/ml
  • normalized fitness= 4.43

OMP_AN:35925

OMP:0006045

increased resistance to beta-lactam compound

Relative to: OMP_AN:27534


Genotype differences:

+lacZ4787(del)( +ynfF736(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Cefoxitin sodium hydrate
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:3501) Cefoxitin sodium hydrate

  • chemical concentration= 0.25 ug/ml
  • normalized fitness= 3.45

OMP_AN:35926

OMP:0006037

increased resistance to macrolide

Relative to: OMP_AN:27519


Genotype differences:

+lacZ4787(del)( +ynfF736(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Clarithromycin
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:3732) Clarithromycin

  • chemical concentration= 5 ug/ml
  • normalized fitness= 3.56

OMP_AN:35927

OMP:0007886

decreased resistance to a metal

Relative to: OMP_AN:27579


Genotype differences:

+lacZ4787(del)( +ynfF736(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Copper stress-CuCl2 dihydrate
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:86318) Copper stress-CuCl2 dihydrate

  • chemical concentration= 4 mM (LB)
  • normalized fitness= -4.31

OMP_AN:35928

OMP:0007913

increased resistance to a chelator

Relative to: OMP_AN:27507


Genotype differences:

+lacZ4787(del)( +ynfF736(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:EDTA disodium salt dihydrate
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:64758) EDTA disodium salt dihydrate

  • chemical concentration= 1 mM
  • normalized fitness= 3.43

OMP_AN:35929

OMP:0007074

increased growth factor requirement

Relative to: OMP_AN:27603


Genotype differences:

+lacZ4787(del)( +ynfF736(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:M9
  • medium:D-Glucose anhydrous
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:42758) D-Glucose anhydrous

  • chemical concentration= 0.2%
  • normalized fitness= 3.6

OMP_AN:35930

OMP:0007071

increased organic carbon source utilization

Relative to: OMP_AN:27613


Genotype differences:

+lacZ4787(del)( +ynfF736(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:M9
  • medium:D-Glucose anhydrous
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:42758) D-Glucose anhydrous

  • chemical concentration= 0.2%
  • normalized fitness= 3.6

OMP_AN:35931

OMP:0007011

increased population growth at alkaline pH

Relative to: OMP_AN:27623


Genotype differences:

+lacZ4787(del)( +ynfF736(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:TAPS
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (**SID:24900259) TAPS

  • chemical concentration= 9.5
  • normalized fitness= 5.85
Showing 1 to 10 of 11 entries


Additional Information

Links

References

See Help:References for how to manage references in OMPwiki.

  1. Jump up Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed OMPwiki page
  2. Jump up to: 2.00 2.01 2.02 2.03 2.04 2.05 2.06 2.07 2.08 2.09 2.10 Nichols, RJ et al. (2011) Phenotypic landscape of a bacterial cell. Cell 144 143-56 PubMed OMPwiki page