OMP ST:74010 ! Escherichia coli K-12 JW4364

From OMPwiki

Strain Summary

Strain Name

Escherichia coli K-12 JW4364

Synonyms

JW4364-1

Taxon Information
  • Pangenome: Escherichia coli
  • Subspecies and/or strain: K-12
  • NCBI Taxonomy ID: 83333
Genotype

DE(araD-araB)567 DE(rhaD-rhaB)568 F- Lambda- hsdR514 lacZ4787(del)(::rrnB-3) rph-1 arcA726(del)::FRT-kan-FRT

Strain Reference
Strain availability

Coli Genetic Stock Center

Ancestry

parent:OMP ST:800 ! Escherichia coli K-12 BW25113

Annotated phenotypes



Notes

Phenotype Annotation

Annotation ID Qualifier OMP ID OMP term name Relative phenotype information Experimental condition ECO ID ECO term name Reference Annotation Extension Notes

OMP_AN:35521

OMP:0007896

decreased resistance to a surfactant

Relative to: OMP_AN:27581


Genotype differences:

+lacZ4787(del)( +arcA726(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Benzalkonium chloride
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:3020) Benzalkonium chloride

  • chemical concentration= 25 ug/ml
  • normalized fitness= -3.59

OMP_AN:35522

OMP:0007879

decreased resistance to an antimicrobial agent

Relative to: OMP_AN:27549


Genotype differences:

+lacZ4787(del)( +arcA726(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:CHIR-090
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:134107 CID:11546620) CHIR-090

  • chemical concentration= 0.02 ug/ml
  • normalized fitness= -3.81

OMP_AN:35523

OMP:0007912

decreased resistance to a chelator

Relative to: OMP_AN:27507


Genotype differences:

+lacZ4787(del)( +arcA726(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:EDTA disodium salt dihydrate
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:64758) EDTA disodium salt dihydrate

  • chemical concentration= 0.1 mM
  • normalized fitness= -4.8

OMP_AN:35524

OMP:0006037

increased resistance to macrolide

Relative to: OMP_AN:27520


Genotype differences:

+lacZ4787(del)( +arcA726(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Erythromycin
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:42355) Erythromycin

  • chemical concentration= 0.1 ug/ml
  • normalized fitness= 3.98

OMP_AN:35525

OMP:0007879

decreased resistance to an antimicrobial agent

Relative to: OMP_AN:27555


Genotype differences:

+lacZ4787(del)( +arcA726(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Fosfomycin disodium salt
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CID:6093162) Fosfomycin disodium salt

  • chemical concentration= 1 ug/ml
  • normalized fitness= -4.4

OMP_AN:35526

OMP:0007377

decreased resistance to an alkylating agent

Relative to: OMP_AN:27502


Genotype differences:

+lacZ4787(del)( +arcA726(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:methyl methanesulfonate (MMS)
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:25255) methyl methanesulfonate (MMS)

  • chemical concentration= 0.05%
  • normalized fitness= -8.77

OMP_AN:35527

OMP:0006040

decreased resistance to quinolone

Relative to: OMP_AN:27543


Genotype differences:

+lacZ4787(del)( +arcA726(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Streptonigrin
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:9287) Streptonigrin

  • chemical concentration= 0.5 ug/ml
  • normalized fitness= -3.28

OMP_AN:35528

OMP:0007902

increased resistance to bile acids

Relative to: OMP_AN:27584


Genotype differences:

+lacZ4787(del)( +arcA726(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Taurocholic acid sodium salt hydrate
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CID:23687511) Taurocholic acid sodium salt hydrate

  • chemical concentration= 1 %
  • normalized fitness= 3.28

OMP_AN:35529

OMP:0007249

decreased resistance to a chemical

Relative to: OMP_AN:27567


Genotype differences:

+lacZ4787(del)( +arcA726(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Theophylline anhydrous
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:28177) Theophylline anhydrous

  • chemical concentration= 100 ug/ml
  • normalized fitness= -4.94

OMP_AN:35530

OMP:0007912

decreased resistance to a chelator

Relative to: OMP_AN:27508


Genotype differences:

+lacZ4787(del)( +arcA726(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:EGTA
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:30740) EGTA

  • chemical concentration= 2 mM
  • normalized fitness= -3.55

OMP_AN:35531

OMP:0007878

increased resistance to an antimicrobial agent

Relative to: OMP_AN:27504


Genotype differences:

+lacZ4787(del)( +arcA726(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Novobiocin sodium salt
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:31924) Novobiocin sodium salt

  • chemical concentration= 30 ug/ml
  • normalized fitness= 3.56

OMP_AN:35532

OMP:0007008

decreased population growth at acidic pH

Relative to: OMP_AN:27622


Genotype differences:

+lacZ4787(del)( +arcA726(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:MES-HOMOPIPES
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (**SID:329817955 SID:57651422) MES-HOMOPIPES

  • chemical concentration= pH 4
  • normalized fitness= -4.79

OMP_AN:35533

OMP:0007009

increased population growth at acidic pH

Relative to: OMP_AN:27622


Genotype differences:

+lacZ4787(del)( +arcA726(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:MES-HOMOPIPES
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (**SID:329817955 SID:57651422) MES-HOMOPIPES

  • chemical concentration= 6
  • normalized fitness= 3.31


Additional Information

Links

References

See Help:References for how to manage references in OMPwiki.

  1. Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed OMPwiki page
  2. 2.00 2.01 2.02 2.03 2.04 2.05 2.06 2.07 2.08 2.09 2.10 2.11 2.12 Nichols, RJ et al. (2011) Phenotypic landscape of a bacterial cell. Cell 144 143-56 PubMed OMPwiki page