OMP ST:73933 ! Escherichia coli K-12 JW4224

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Strain Summary

Strain Name

Escherichia coli K-12 JW4224

Synonyms

JW4224-1

Taxon Information
  • Pangenome: Escherichia coli
  • Subspecies and/or strain: K-12
  • NCBI Taxonomy ID: 83333
Genotype

DE(araD-araB)567 DE(rhaD-rhaB)568 F- Lambda- hsdR514 lacZ4787(del)(::rrnB-3) rph-1 idnD738(del)::FRT-kan-FRT

Strain Reference
Strain availability

Coli Genetic Stock Center

Ancestry

parent:OMP ST:800 ! Escherichia coli K-12 BW25113

Annotated phenotypes


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Notes

Phenotype Annotation

Showing 1 to 10 of 11 entries
Annotation IDQualifierOMP IDOMP term nameRelative phenotype informationExperimental conditionECO IDECO term nameReferenceAnnotation ExtensionNotes

OMP_AN:35389

OMP:0007075

decreased growth factor requirement

Relative to: OMP_AN:27601

Genotype differences:

+lacZ4787(del)( +idnD738(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:M9
  • medium:Sodium Acetate anhydrous
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:32954) Sodium Acetate anhydrous

  • chemical concentration= 0.6%
  • normalized fitness= -6.11

OMP_AN:35390

OMP:0007070

decreased organic carbon source utilization

Relative to: OMP_AN:27611

Genotype differences:

+lacZ4787(del)( +idnD738(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:M9
  • medium:Sodium Acetate anhydrous
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:32954) Sodium Acetate anhydrous

  • chemical concentration= 0.6%
  • normalized fitness= -6.11

OMP_AN:35391

OMP:0006044

decreased resistance to beta-lactam

Relative to: OMP_AN:27534

Genotype differences:

+lacZ4787(del)( +idnD738(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Cefoxitin sodium hydrate
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:3501) Cefoxitin sodium hydrate

  • chemical concentration= 0.5 ug/ml
  • normalized fitness= -3.86

OMP_AN:35392

OMP:0007250

increased resistance to a chemical

Relative to: OMP_AN:27514

Genotype differences:

+lacZ4787(del)( +idnD738(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Chlorpromazine hydrochloride
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:3649) Chlorpromazine hydrochloride

  • chemical concentration= 12 uM
  • normalized fitness= 4.08

OMP_AN:35393

OMP:0007903

decreased resistance to bile acids

Relative to: OMP_AN:27586

Genotype differences:

+lacZ4787(del)( +idnD738(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Deoxycholate sodium
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:9177) Deoxycholate sodium

  • chemical concentration= 0.1 %
  • normalized fitness= -5.79

OMP_AN:35394

OMP:0007249

decreased resistance to a chemical

Relative to: OMP_AN:27572

Genotype differences:

+lacZ4787(del)( +idnD738(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Epigallocatechin gallate (EGCG)
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:4806) Epigallocatechin gallate (EGCG)

  • chemical concentration= 50 uM
  • normalized fitness= -3.49

OMP_AN:35395

OMP:0007075

decreased growth factor requirement

Relative to: OMP_AN:27604

Genotype differences:

+lacZ4787(del)( +idnD738(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:M9
  • medium:Glycerol
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:17754) Glycerol

  • chemical concentration= 0.4%
  • normalized fitness= -3.49

OMP_AN:35396

OMP:0007070

decreased organic carbon source utilization

Relative to: OMP_AN:27614

Genotype differences:

+lacZ4787(del)( +idnD738(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:M9
  • medium:Glycerol
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:17754) Glycerol

  • chemical concentration= 0.4%
  • normalized fitness= -3.49

OMP_AN:35397

OMP:0007249

decreased resistance to a chemical

Relative to: OMP_AN:27573

Genotype differences:

+lacZ4787(del)( +idnD738(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Procaine hydrochloride
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:8431) Procaine hydrochloride

  • chemical concentration= 1 mM
  • normalized fitness= -5

OMP_AN:35398

OMP:0007896

decreased resistance to a surfactant

Relative to: OMP_AN:27583

Genotype differences:

+lacZ4787(del)( +idnD738(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Triton X-100
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:9750) Triton X-100

  • chemical concentration= 0.03 %
  • normalized fitness= -4.11
Showing 1 to 10 of 11 entries
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Additional Information

Links

References

See Help:References for how to manage references in OMPwiki.

  1. Jump up ↑ Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed OMPwiki page
  2. ↑ Jump up to: 2.00 2.01 2.02 2.03 2.04 2.05 2.06 2.07 2.08 2.09 2.10 Nichols, RJ et al. (2011) Phenotypic landscape of a bacterial cell. Cell 144 143-56 PubMed OMPwiki page
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