OMP ST:73850 ! Escherichia coli K-12 JW4099

From OMPwiki

Strain Summary

Strain Name

Escherichia coli K-12 JW4099

Synonyms

JW4099-1

Taxon Information
  • Pangenome: Escherichia coli
  • Subspecies and/or strain: K-12
  • NCBI Taxonomy ID: 83333
Genotype

DE(araD-araB)567 DE(rhaD-rhaB)568 F- Lambda- hsdR514 lacZ4787(del)(::rrnB-3) rph-1 aspA765(del)::FRT-kan-FRT

Strain Reference
Strain availability

Coli Genetic Stock Center

Ancestry

parent:OMP ST:800 ! Escherichia coli K-12 BW25113

Annotated phenotypes



Notes

Phenotype Annotation

Annotation ID Qualifier OMP ID OMP term name Relative phenotype information Experimental condition ECO ID ECO term name Reference Annotation Extension Notes

OMP_AN:35091

OMP:0007953

increased population growth at high temperature

Relative to: OMP_AN:27621


Genotype differences:

+lacZ4787(del)( +aspA765(del) -lacZ4787(del)


Condition differences:
  • temperature:45C
  • medium37C
  • medium
  • temperature:45C
  • medium:LB agar
  • medium:High temperature
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

  • chemical concentration= 45 C
  • normalized fitness= 3.44

OMP_AN:35092

OMP:0007878

increased resistance to an antimicrobial agent

Relative to: OMP_AN:27557


Genotype differences:

+lacZ4787(del)( +aspA765(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:A22
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CID:2830968) A22

  • chemical concentration= 2 ug/ml
  • normalized fitness= 3.61

OMP_AN:35093

OMP:0007074

increased growth factor requirement

Relative to: OMP_AN:27601


Genotype differences:

+lacZ4787(del)( +aspA765(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:M9
  • medium:Sodium Acetate anhydrous
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:32954) Sodium Acetate anhydrous

  • chemical concentration= 0.6%
  • normalized fitness= 3.36

OMP_AN:35094

OMP:0007071

increased organic carbon source utilization

Relative to: OMP_AN:27611


Genotype differences:

+lacZ4787(del)( +aspA765(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:M9
  • medium:Sodium Acetate anhydrous
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:32954) Sodium Acetate anhydrous

  • chemical concentration= 0.6%
  • normalized fitness= 3.36

OMP_AN:35095

OMP:0006044

decreased resistance to beta-lactam

Relative to: OMP_AN:27530


Genotype differences:

+lacZ4787(del)( +aspA765(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Amoxicillin
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:2676) Amoxicillin

  • chemical concentration= 1 ug/ml
  • normalized fitness= -4.61

OMP_AN:35096

OMP:0007879

decreased resistance to an antimicrobial agent

Relative to: OMP_AN:27552


Genotype differences:

+lacZ4787(del)( +aspA765(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Azidothymidine
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:10110) Azidothymidine

  • chemical concentration= 1 ng/ml
  • normalized fitness= -4.38

OMP_AN:35097

OMP:0006044

decreased resistance to beta-lactam

Relative to: OMP_AN:27534


Genotype differences:

+lacZ4787(del)( +aspA765(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Cefoxitin sodium hydrate
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:3501) Cefoxitin sodium hydrate

  • chemical concentration= 0.25 ug/ml
  • normalized fitness= -6.73

OMP_AN:35098

OMP:0007879

decreased resistance to an antimicrobial agent

Relative to: OMP_AN:27548


Genotype differences:

+lacZ4787(del)( +aspA765(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Cerulenin
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:171741) Cerulenin

  • chemical concentration= 6 ug/ml
  • normalized fitness= -4.18

OMP_AN:35099

OMP:0007903

decreased resistance to bile acids

Relative to: OMP_AN:27585


Genotype differences:

+lacZ4787(del)( +aspA765(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Cholate sodium hydrate
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CID:23679061) Cholate sodium hydrate

  • chemical concentration= 0.5 %
  • normalized fitness= -3.36

OMP_AN:35100

OMP:0007249

decreased resistance to a chemical

Relative to: OMP_AN:27509


Genotype differences:

+lacZ4787(del)( +aspA765(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Cisplatin-cis-Diamineplatinum(II) dichloride
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:27899) Cisplatin-cis-Diamineplatinum(II) dichloride

  • chemical concentration= 100 ug/ml
  • normalized fitness= -3.57

OMP_AN:35101

OMP:0007902

increased resistance to bile acids

Relative to: OMP_AN:27586


Genotype differences:

+lacZ4787(del)( +aspA765(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Deoxycholate sodium
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:9177) Deoxycholate sodium

  • chemical concentration= 2 %
  • normalized fitness= 3.17

OMP_AN:35102

OMP:0007249

decreased resistance to a chemical

Relative to: OMP_AN:27501


Genotype differences:

+lacZ4787(del)( +aspA765(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Dibucaine
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:247956) Dibucaine

  • chemical concentration= 1.2 mM
  • normalized fitness= -6.23

OMP_AN:35103

OMP:0007249

decreased resistance to a chemical

Relative to: OMP_AN:27572


Genotype differences:

+lacZ4787(del)( +aspA765(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Epigallocatechin gallate (EGCG)
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:4806) Epigallocatechin gallate (EGCG)

  • chemical concentration= 20 uM
  • normalized fitness= -4.78

OMP_AN:35104

OMP:0007250

increased resistance to a chemical

Relative to: OMP_AN:27572


Genotype differences:

+lacZ4787(del)( +aspA765(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Epigallocatechin gallate (EGCG)
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:4806) Epigallocatechin gallate (EGCG)

  • chemical concentration= 50 uM
  • normalized fitness= 3.35

OMP_AN:35105

OMP:0007879

decreased resistance to an antimicrobial agent

Relative to: OMP_AN:27550


Genotype differences:

+lacZ4787(del)( +aspA765(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Ethidium Bromide
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:4883) Ethidium Bromide

  • chemical concentration= 10 ug/ml
  • normalized fitness= -4.9

OMP_AN:35106

OMP:0007074

increased growth factor requirement

Relative to: OMP_AN:27603


Genotype differences:

+lacZ4787(del)( +aspA765(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:M9
  • medium:D-Glucose anhydrous
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:42758) D-Glucose anhydrous

  • chemical concentration= 0.2%
  • normalized fitness= 3.88

OMP_AN:35107

OMP:0007071

increased organic carbon source utilization

Relative to: OMP_AN:27613


Genotype differences:

+lacZ4787(del)( +aspA765(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:M9
  • medium:D-Glucose anhydrous
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:42758) D-Glucose anhydrous

  • chemical concentration= 0.2%
  • normalized fitness= 3.88

OMP_AN:35108

OMP:0007074

increased growth factor requirement

Relative to: OMP_AN:27604


Genotype differences:

+lacZ4787(del)( +aspA765(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:M9
  • medium:Glycerol
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:17754) Glycerol

  • chemical concentration= 0.4%
  • normalized fitness= 3.41

OMP_AN:35109

OMP:0007071

increased organic carbon source utilization

Relative to: OMP_AN:27614


Genotype differences:

+lacZ4787(del)( +aspA765(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:M9
  • medium:Glycerol
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:17754) Glycerol

  • chemical concentration= 0.4%
  • normalized fitness= 3.41

OMP_AN:35110

OMP:0006040

decreased resistance to quinolone

Relative to: OMP_AN:27540


Genotype differences:

+lacZ4787(del)( +aspA765(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Levofloxacin
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:63598) Levofloxacin

  • chemical concentration= 0.002 ug/ml
  • normalized fitness= -3.64

OMP_AN:35111

OMP:0007074

increased growth factor requirement

Relative to: OMP_AN:27605


Genotype differences:

+lacZ4787(del)( +aspA765(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:M9
  • medium:D-(+) Maltose monohydrate
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CID:23615261) D-(+) Maltose monohydrate

  • chemical concentration= 0.1%
  • normalized fitness= 3.14

OMP_AN:35112

OMP:0007071

increased organic carbon source utilization

Relative to: OMP_AN:27615


Genotype differences:

+lacZ4787(del)( +aspA765(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:M9
  • medium:D-(+) Maltose monohydrate
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CID:23615261) D-(+) Maltose monohydrate

  • chemical concentration= 0.1%
  • normalized fitness= 3.14

OMP_AN:35113

OMP:0007249

decreased resistance to a chemical

Relative to: OMP_AN:27505


Genotype differences:

+lacZ4787(del)( +aspA765(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Methotrexate hydrate
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CID:165528) Methotrexate hydrate

  • chemical concentration= 1 ug/ml
  • normalized fitness= -4.09

OMP_AN:35114

OMP:0007249

decreased resistance to a chemical

Relative to: OMP_AN:27510


Genotype differences:

+lacZ4787(del)( +aspA765(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Mitomycin C
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:27504) Mitomycin C

  • chemical concentration= 0.1 ug/ml
  • normalized fitness= -8.02

OMP_AN:35115

OMP:0007074

increased growth factor requirement

Relative to: OMP_AN:27600


Genotype differences:

+lacZ4787(del)( +aspA765(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:MOPS
  • medium:NH4Cl
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:31206) NH4Cl

  • chemical concentration= 9.5mm
  • normalized fitness= 3.92

OMP_AN:35116

OMP:0007033

increased utilization of inorganic nitrogen source

Relative to: OMP_AN:27610


Genotype differences:

+lacZ4787(del)( +aspA765(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:MOPS
  • medium:NH4Cl
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:31206) NH4Cl

  • chemical concentration= 9.5mm
  • normalized fitness= 3.92

OMP_AN:35117

OMP:0007249

decreased resistance to a chemical

Relative to: OMP_AN:27513


Genotype differences:

+lacZ4787(del)( +aspA765(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Phenazine methosulfate (PMS)
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:8055) Phenazine methosulfate (PMS)

  • chemical concentration= 0.1 mM
  • normalized fitness= -3.44

OMP_AN:35118

OMP:0007250

increased resistance to a chemical

Relative to: OMP_AN:27573


Genotype differences:

+lacZ4787(del)( +aspA765(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Procaine hydrochloride
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:8431) Procaine hydrochloride

  • chemical concentration= 10 mM
  • normalized fitness= 4.06

OMP_AN:35119

OMP:0007903

decreased resistance to bile acids

Relative to: OMP_AN:27584


Genotype differences:

+lacZ4787(del)( +aspA765(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Taurocholic acid sodium salt hydrate
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CID:23687511) Taurocholic acid sodium salt hydrate

  • chemical concentration= 0.5 %
  • normalized fitness= -6.81

OMP_AN:35120

OMP:0006058

increased resistance to aminoglycoside

Relative to: OMP_AN:27529


Genotype differences:

+lacZ4787(del)( +aspA765(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Tobramycin
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:28864) Tobramycin

  • chemical concentration= 0.1 ug/ml
  • normalized fitness= 3.78

OMP_AN:35121

OMP:0007896

decreased resistance to a surfactant

Relative to: OMP_AN:27583


Genotype differences:

+lacZ4787(del)( +aspA765(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Triton X-100
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:9750) Triton X-100

  • chemical concentration= 0.03 %
  • normalized fitness= -6.76

OMP_AN:35122

OMP:0006044

decreased resistance to beta-lactam

Relative to: OMP_AN:27532


Genotype differences:

+lacZ4787(del)( +aspA765(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Aztreonam
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:161680) Aztreonam

  • chemical concentration= 0.04 ug/ml
  • normalized fitness= -3.63

OMP_AN:35123

OMP:0006044

decreased resistance to beta-lactam

Relative to: OMP_AN:27536


Genotype differences:

+lacZ4787(del)( +aspA765(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Ceftazidime hydrate
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:3509) Ceftazidime hydrate

  • chemical concentration= 0.05 ug/ml
  • normalized fitness= -4.12

OMP_AN:35124

OMP:0006057

decreased resistance to aminoglycoside

Relative to: OMP_AN:27526


Genotype differences:

+lacZ4787(del)( +aspA765(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Gentamicin sulfate salt
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:5312) Gentamicin sulfate salt

  • chemical concentration= 0.05 ug/ml
  • normalized fitness= -3.7

OMP_AN:35125

OMP:0007074

increased growth factor requirement

Relative to: OMP_AN:27598


Genotype differences:

+lacZ4787(del)( +aspA765(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:MOPS
  • medium:Iron excess-FeSO4 heptahydride
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:75836) Iron excess-FeSO4 heptahydride

  • chemical concentration= 1mM (MOPS; normal 100uM)
  • normalized fitness= 3.38

OMP_AN:35126

OMP:0007888

increased resistance to a metal

Relative to: OMP_AN:27608


Genotype differences:

+lacZ4787(del)( +aspA765(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:MOPS
  • medium:Iron excess-FeSO4 heptahydride
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:75836) Iron excess-FeSO4 heptahydride

  • chemical concentration= 1mM (MOPS; normal 100uM)
  • normalized fitness= 3.38

OMP_AN:35127

OMP:0007074

increased growth factor requirement

Relative to: OMP_AN:27599


Genotype differences:

+lacZ4787(del)( +aspA765(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:MOPS
  • medium:Iron starvation-FeSO4·7H2O
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:75836) Iron starvation-FeSO4·7H2O

  • chemical concentration= 2uM (MOPS; normal 100uM)
  • normalized fitness= 3.76

OMP_AN:35128

OMP:0007936

increased resistance to nutrient limitation

Relative to: OMP_AN:27609


Genotype differences:

+lacZ4787(del)( +aspA765(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:MOPS
  • medium:Iron starvation-FeSO4·7H2O
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:75836) Iron starvation-FeSO4·7H2O

  • chemical concentration= 2uM (MOPS; normal 100uM)
  • normalized fitness= 3.76

OMP_AN:35129

OMP:0007879

decreased resistance to an antimicrobial agent

Relative to: OMP_AN:27504


Genotype differences:

+lacZ4787(del)( +aspA765(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Novobiocin sodium salt
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:31924) Novobiocin sodium salt

  • chemical concentration= 6 ug/ml
  • normalized fitness= -6.16

OMP_AN:35130

OMP:0006036

decreased resistance to macrolide

Relative to: OMP_AN:27521


Genotype differences:

+lacZ4787(del)( +aspA765(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Spiramycin
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CID:6419898) Spiramycin

  • chemical concentration= 5 ug/ml
  • normalized fitness= -5.3

OMP_AN:35131

OMP:0006063

increased resistance to tetracycline

Relative to: OMP_AN:27524


Genotype differences:

+lacZ4787(del)( +aspA765(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Tetracycline hydrochloride
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:35006) Tetracycline hydrochloride

  • chemical concentration= 0.5 ug/ml
  • normalized fitness= 4.6


Additional Information

Links

References

See Help:References for how to manage references in OMPwiki.

  1. Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed OMPwiki page
  2. 2.00 2.01 2.02 2.03 2.04 2.05 2.06 2.07 2.08 2.09 2.10 2.11 2.12 2.13 2.14 2.15 2.16 2.17 2.18 2.19 2.20 2.21 2.22 2.23 2.24 2.25 2.26 2.27 2.28 2.29 2.30 2.31 2.32 2.33 2.34 2.35 2.36 2.37 2.38 2.39 2.40 Nichols, RJ et al. (2011) Phenotypic landscape of a bacterial cell. Cell 144 143-56 PubMed OMPwiki page