OMP ST:73713 ! Escherichia coli K-12 JW3907

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Strain Summary

Strain Name

Escherichia coli K-12 JW3907

Synonyms

JW3907-1

Taxon Information
  • Pangenome: Escherichia coli
  • Subspecies and/or strain: K-12
  • NCBI Taxonomy ID: 83333
Genotype

DE(araD-araB)567 DE(rhaD-rhaB)568 F- Lambda- hsdR514 lacZ4787(del)(::rrnB-3) rph-1 rpmE723(del)::FRT-kan-FRT

Strain Reference
Strain availability

Coli Genetic Stock Center

Ancestry

parent:OMP ST:800 ! Escherichia coli K-12 BW25113

Annotated phenotypes


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Notes

Phenotype Annotation

Annotation ID Qualifier OMP ID OMP term name Relative phenotype information Experimental condition ECO ID ECO term name Reference Annotation Extension Notes

OMP_AN:34618

OMP:0007879

decreased resistance to an antimicrobial agent

Relative to: OMP_AN:27552

Genotype differences:

+lacZ4787(del)( +rpmE723(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Azidothymidine
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:10110) Azidothymidine

  • chemical concentration= 2.5 ng/ml
  • normalized fitness= -3.15

OMP_AN:34619

OMP:0006036

decreased resistance to macrolide

Relative to: OMP_AN:27518

Genotype differences:

+lacZ4787(del)( +rpmE723(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Azithromycin
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:2955) Azithromycin

  • chemical concentration= 0.1 ug/ml
  • normalized fitness= -4.46

OMP_AN:34620

OMP:0006036

decreased resistance to macrolide

Relative to: OMP_AN:27519

Genotype differences:

+lacZ4787(del)( +rpmE723(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Clarithromycin
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:3732) Clarithromycin

  • chemical concentration= 10 ug/ml
  • normalized fitness= -3.42

OMP_AN:34621

OMP:0007886

decreased resistance to a metal

Relative to: OMP_AN:27579

Genotype differences:

+lacZ4787(del)( +rpmE723(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Copper stress-CuCl2 dihydrate
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:86318) Copper stress-CuCl2 dihydrate

  • chemical concentration= 4 mM (LB)
  • normalized fitness= -4.01

OMP_AN:34622

OMP:0006040

decreased resistance to quinolone

Relative to: OMP_AN:27543

Genotype differences:

+lacZ4787(del)( +rpmE723(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Streptonigrin
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:9287) Streptonigrin

  • chemical concentration= 0.2 ug/ml
  • normalized fitness= -4.25

OMP_AN:34623

OMP:0007250

increased resistance to a chemical

Relative to: OMP_AN:27568

Genotype differences:

+lacZ4787(del)( +rpmE723(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:(±)-Verapamil hydrochloride
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:53188) (±)-Verapamil hydrochloride

  • chemical concentration= 1 mM
  • normalized fitness= 3.51

OMP_AN:34624

OMP:0006061

decreased resistance to tetracycline

Relative to: OMP_AN:27522

Genotype differences:

+lacZ4787(del)( +rpmE723(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Doxycycline hyclate
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:34730) Doxycycline hyclate

  • chemical concentration= 1 ug/ml
  • normalized fitness= -3.8

OMP_AN:34625

OMP:0007521

decreased population growth in high osmolarity

Relative to: OMP_AN:27595

Genotype differences:

+lacZ4787(del)( +rpmE723(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:NaCl
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:26710) NaCl

  • chemical concentration= 150 mM
  • normalized fitness= -4.29

OMP_AN:34626

OMP:0006061

decreased resistance to tetracycline

Relative to: OMP_AN:27524

Genotype differences:

+lacZ4787(del)( +rpmE723(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Tetracycline hydrochloride
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:35006) Tetracycline hydrochloride

  • chemical concentration= 0.5 ug/ml
  • normalized fitness= -4.29
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Additional Information

Links

References

See Help:References for how to manage references in OMPwiki.

  1. ↑ Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed OMPwiki page
  2. ↑ 2.0 2.1 2.2 2.3 2.4 2.5 2.6 2.7 2.8 Nichols, RJ et al. (2011) Phenotypic landscape of a bacterial cell. Cell 144 143-56 PubMed OMPwiki page
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