OMP ST:72889 ! Escherichia coli K-12 JW2541

From OMPwiki

Strain Summary

Strain Name

Escherichia coli K-12 JW2541

Synonyms

JW2541-1

Taxon Information
  • Pangenome: Escherichia coli
  • Subspecies and/or strain: K-12
  • NCBI Taxonomy ID: 83333
Genotype

DE(araD-araB)567 DE(rhaD-rhaB)568 F- Lambda- hsdR514 lacZ4787(del)(::rrnB-3) rph-1 purL731(del)::FRT-kan-FRT

Strain Reference
Strain availability

Coli Genetic Stock Center

Ancestry

parent:OMP ST:800 ! Escherichia coli K-12 BW25113

Annotated phenotypes



Notes

Phenotype Annotation

Annotation ID Qualifier OMP ID OMP term name Relative phenotype information Experimental condition ECO ID ECO term name Reference Annotation Extension Notes

OMP_AN:32040

OMP:0007075

decreased growth factor requirement

Relative to: OMP_AN:27601


Genotype differences:

+lacZ4787(del)( +purL731(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:M9
  • medium:Sodium Acetate anhydrous
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:32954) Sodium Acetate anhydrous

  • chemical concentration= 0.6%
  • normalized fitness= -21.71

OMP_AN:32041

OMP:0007070

decreased organic carbon source utilization

Relative to: OMP_AN:27611


Genotype differences:

+lacZ4787(del)( +purL731(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:M9
  • medium:Sodium Acetate anhydrous
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:32954) Sodium Acetate anhydrous

  • chemical concentration= 0.6%
  • normalized fitness= -21.71

OMP_AN:32042

OMP:0006044

decreased resistance to beta-lactam

Relative to: OMP_AN:27530


Genotype differences:

+lacZ4787(del)( +purL731(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Amoxicillin
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:2676) Amoxicillin

  • chemical concentration= 1 ug/ml
  • normalized fitness= -4.23

OMP_AN:32043

OMP:0007879

decreased resistance to an antimicrobial agent

Relative to: OMP_AN:27552


Genotype differences:

+lacZ4787(del)( +purL731(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Azidothymidine
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:10110) Azidothymidine

  • chemical concentration= 1 ng/ml
  • normalized fitness= -3.33

OMP_AN:32044

OMP:0007903

decreased resistance to bile acids

Relative to: OMP_AN:27587


Genotype differences:

+lacZ4787(del)( +purL731(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Bile salts chemical-Mixture of sodium cholate (CHEBI:26711) and sodium deoxycholate (CHEBI:9177)
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:26711 + CHEBI:9177) Bile salts chemical-Mixture of sodium cholate (CHEBI:26711) and sodium deoxycholate (CHEBI:9177)

  • chemical concentration= 0.1 %
  • normalized fitness= -9.04

OMP_AN:32045

OMP:0007250

increased resistance to a chemical

Relative to: OMP_AN:27571


Genotype differences:

+lacZ4787(del)( +purL731(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:CCCP (Carbonyl cyanide 3-chlorophenylhydrazone)
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:3259) CCCP (Carbonyl cyanide 3-chlorophenylhydrazone)

  • chemical concentration= 0.5 ug/ml
  • normalized fitness= 4.12

OMP_AN:32046

OMP:0007879

decreased resistance to an antimicrobial agent

Relative to: OMP_AN:27549


Genotype differences:

+lacZ4787(del)( +purL731(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:CHIR-090
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:134107 CID:11546620) CHIR-090

  • chemical concentration= 0.025 ug/ml
  • normalized fitness= -5.91

OMP_AN:32047

OMP:0006036

decreased resistance to macrolide

Relative to: OMP_AN:27519


Genotype differences:

+lacZ4787(del)( +purL731(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Clarithromycin
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:3732) Clarithromycin

  • chemical concentration= 0.1 ug/ml
  • normalized fitness= -3.62

OMP_AN:32048

OMP:0007903

decreased resistance to bile acids

Relative to: OMP_AN:27586


Genotype differences:

+lacZ4787(del)( +purL731(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Deoxycholate sodium
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:9177) Deoxycholate sodium

  • chemical concentration= 2 %
  • normalized fitness= -11.56

OMP_AN:32049

OMP:0007075

decreased growth factor requirement

Relative to: OMP_AN:27606


Genotype differences:

+lacZ4787(del)( +purL731(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:M9
  • medium:N-Acetyl-D-glucosamine
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:506227) N-Acetyl-D-glucosamine

  • chemical concentration= 0.15%
  • normalized fitness= -22.45

OMP_AN:32050

OMP:0007070

decreased organic carbon source utilization

Relative to: OMP_AN:27616


Genotype differences:

+lacZ4787(del)( +purL731(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:M9
  • medium:N-Acetyl-D-glucosamine
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:506227) N-Acetyl-D-glucosamine

  • chemical concentration= 0.15%
  • normalized fitness= -22.45

OMP_AN:32051

OMP:0007075

decreased growth factor requirement

Relative to: OMP_AN:27602


Genotype differences:

+lacZ4787(del)( +purL731(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:M9
  • medium:D-(+) Glucosamine hydrochloride
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CID:91431) D-(+) Glucosamine hydrochloride

  • chemical concentration= 0.2%
  • normalized fitness= -16.54

OMP_AN:32052

OMP:0007070

decreased organic carbon source utilization

Relative to: OMP_AN:27612


Genotype differences:

+lacZ4787(del)( +purL731(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:M9
  • medium:D-(+) Glucosamine hydrochloride
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CID:91431) D-(+) Glucosamine hydrochloride

  • chemical concentration= 0.2%
  • normalized fitness= -16.54

OMP_AN:32053

OMP:0007075

decreased growth factor requirement

Relative to: OMP_AN:27603


Genotype differences:

+lacZ4787(del)( +purL731(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:M9
  • medium:D-Glucose anhydrous
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:42758) D-Glucose anhydrous

  • chemical concentration= 0.2%
  • normalized fitness= -15.22

OMP_AN:32054

OMP:0007070

decreased organic carbon source utilization

Relative to: OMP_AN:27613


Genotype differences:

+lacZ4787(del)( +purL731(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:M9
  • medium:D-Glucose anhydrous
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:42758) D-Glucose anhydrous

  • chemical concentration= 0.2%
  • normalized fitness= -15.22

OMP_AN:32055

OMP:0007075

decreased growth factor requirement

Relative to: OMP_AN:27604


Genotype differences:

+lacZ4787(del)( +purL731(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:M9
  • medium:Glycerol
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:17754) Glycerol

  • chemical concentration= 0.4%
  • normalized fitness= -18.59

OMP_AN:32056

OMP:0007070

decreased organic carbon source utilization

Relative to: OMP_AN:27614


Genotype differences:

+lacZ4787(del)( +purL731(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:M9
  • medium:Glycerol
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:17754) Glycerol

  • chemical concentration= 0.4%
  • normalized fitness= -18.59

OMP_AN:32057

OMP:0007174

increased resistance to a host defense peptide

Relative to: OMP_AN:27563


Genotype differences:

+lacZ4787(del)( +purL731(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Indolicidin
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (UniProtKB:P33046) Indolicidin

  • chemical concentration= 0.1 ug/ml
  • normalized fitness= 4.22

OMP_AN:32058

OMP:0007075

decreased growth factor requirement

Relative to: OMP_AN:27605


Genotype differences:

+lacZ4787(del)( +purL731(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:M9
  • medium:D-(+) Maltose monohydrate
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CID:23615261) D-(+) Maltose monohydrate

  • chemical concentration= 0.1%
  • normalized fitness= -15.05

OMP_AN:32059

OMP:0007070

decreased organic carbon source utilization

Relative to: OMP_AN:27615


Genotype differences:

+lacZ4787(del)( +purL731(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:M9
  • medium:D-(+) Maltose monohydrate
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CID:23615261) D-(+) Maltose monohydrate

  • chemical concentration= 0.1%
  • normalized fitness= -15.05

OMP_AN:32060

OMP:0007075

decreased growth factor requirement

Relative to: OMP_AN:27600


Genotype differences:

+lacZ4787(del)( +purL731(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:MOPS
  • medium:NH4Cl
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:31206) NH4Cl

  • chemical concentration= 9.5mm
  • normalized fitness= -24.44

OMP_AN:32061

OMP:0007032

decreased utilization of inorganic nitrogen source

Relative to: OMP_AN:27610


Genotype differences:

+lacZ4787(del)( +purL731(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:MOPS
  • medium:NH4Cl
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:31206) NH4Cl

  • chemical concentration= 9.5mm
  • normalized fitness= -24.44

OMP_AN:32062

OMP:0007879

decreased resistance to an antimicrobial agent

Relative to: OMP_AN:27503


Genotype differences:

+lacZ4787(del)( +purL731(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Nitrofurantoin
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:71415) Nitrofurantoin

  • chemical concentration= 1.5 ug/ml
  • normalized fitness= -9.44

OMP_AN:32063

OMP:0007249

decreased resistance to a chemical

Relative to: OMP_AN:27574


Genotype differences:

+lacZ4787(del)( +purL731(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Paraquat dichloride
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:28786) Paraquat dichloride

  • chemical concentration= 5 uM
  • normalized fitness= -7.51

OMP_AN:32064

OMP:0007075

decreased growth factor requirement

Relative to: OMP_AN:27607


Genotype differences:

+lacZ4787(del)( +purL731(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:M9
  • medium:Succinic acid
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:15741) Succinic acid

  • chemical concentration= 0.3%
  • normalized fitness= -13.1

OMP_AN:32065

OMP:0007070

decreased organic carbon source utilization

Relative to: OMP_AN:27617


Genotype differences:

+lacZ4787(del)( +purL731(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:M9
  • medium:Succinic acid
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:15741) Succinic acid

  • chemical concentration= 0.3%
  • normalized fitness= -13.1

OMP_AN:32066

OMP:0007895

increased resistance to a surfactant

Relative to: OMP_AN:27583


Genotype differences:

+lacZ4787(del)( +purL731(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Triton X-100
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:9750) Triton X-100

  • chemical concentration= 0.2 %
  • normalized fitness= 4.07

OMP_AN:32067

OMP:0006044

decreased resistance to beta-lactam

Relative to: OMP_AN:27532


Genotype differences:

+lacZ4787(del)( +purL731(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Aztreonam
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:161680) Aztreonam

  • chemical concentration= 0.04 ug/ml
  • normalized fitness= -3.66

OMP_AN:32068

OMP:0007879

decreased resistance to an antimicrobial agent

Relative to: OMP_AN:27560


Genotype differences:

+lacZ4787(del)( +purL731(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Bacitracin Zinc Sulfate
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CID:16219032) Bacitracin Zinc Sulfate

  • chemical concentration= 300 ug/ml
  • normalized fitness= -3.68

OMP_AN:32069

OMP:0007075

decreased growth factor requirement

Relative to: OMP_AN:27598


Genotype differences:

+lacZ4787(del)( +purL731(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:MOPS
  • medium:Iron excess-FeSO4 heptahydride
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:75836) Iron excess-FeSO4 heptahydride

  • chemical concentration= 1mM (MOPS; normal 100uM)
  • normalized fitness= -22.9

OMP_AN:32070

OMP:0007886

decreased resistance to a metal

Relative to: OMP_AN:27608


Genotype differences:

+lacZ4787(del)( +purL731(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:MOPS
  • medium:Iron excess-FeSO4 heptahydride
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:75836) Iron excess-FeSO4 heptahydride

  • chemical concentration= 1mM (MOPS; normal 100uM)
  • normalized fitness= -22.9

OMP_AN:32071

OMP:0007075

decreased growth factor requirement

Relative to: OMP_AN:27599


Genotype differences:

+lacZ4787(del)( +purL731(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:MOPS
  • medium:Iron starvation-FeSO4·7H2O
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:75836) Iron starvation-FeSO4·7H2O

  • chemical concentration= 2uM (MOPS; normal 100uM)
  • normalized fitness= -20.69

OMP_AN:32072

OMP:0007937

decreased resistance to nutrient limitation

Relative to: OMP_AN:27609


Genotype differences:

+lacZ4787(del)( +purL731(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:MOPS
  • medium:Iron starvation-FeSO4·7H2O
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:75836) Iron starvation-FeSO4·7H2O

  • chemical concentration= 2uM (MOPS; normal 100uM)
  • normalized fitness= -20.69

OMP_AN:32073

OMP:0007009

increased population growth at acidic pH

Relative to: OMP_AN:27622


Genotype differences:

+lacZ4787(del)( +purL731(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:MES-HOMOPIPES
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (**SID:329817955 SID:57651422) MES-HOMOPIPES

  • chemical concentration= pH 4
  • normalized fitness= 3.17

OMP_AN:32074

OMP:0007008

decreased population growth at acidic pH

Relative to: OMP_AN:27622


Genotype differences:

+lacZ4787(del)( +purL731(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:MES-HOMOPIPES
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (**SID:329817955 SID:57651422) MES-HOMOPIPES

  • chemical concentration= 6
  • normalized fitness= -5.17

OMP_AN:32075

OMP:0007010

decreased population growth at alkaline pH

Relative to: OMP_AN:27623


Genotype differences:

+lacZ4787(del)( +purL731(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:TAPS
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (**SID:24900259) TAPS

  • chemical concentration= 10
  • normalized fitness= -5.02


Additional Information

Links

References

See Help:References for how to manage references in OMPwiki.

  1. Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed OMPwiki page
  2. 2.00 2.01 2.02 2.03 2.04 2.05 2.06 2.07 2.08 2.09 2.10 2.11 2.12 2.13 2.14 2.15 2.16 2.17 2.18 2.19 2.20 2.21 2.22 2.23 2.24 2.25 2.26 2.27 2.28 2.29 2.30 2.31 2.32 2.33 2.34 2.35 Nichols, RJ et al. (2011) Phenotypic landscape of a bacterial cell. Cell 144 143-56 PubMed OMPwiki page