OMP ST:72794 ! Escherichia coli K-12 JW2414

From OMPwiki

Strain Summary

Strain Name

Escherichia coli K-12 JW2414

Synonyms

JW2414-3

Taxon Information
  • Pangenome: Escherichia coli
  • Subspecies and/or strain: K-12
  • NCBI Taxonomy ID: 83333
Genotype

DE(araD-araB)567 DE(rhaD-rhaB)568 F- Lambda- hsdR514 lacZ4787(del)(::rrnB-3) rph-1 cysM750(del)::FRT-kan-FRT

Strain Reference
Strain availability

Coli Genetic Stock Center

Ancestry

parent:OMP ST:800 ! Escherichia coli K-12 BW25113

Annotated phenotypes



Notes

Phenotype Annotation

Showing 1 to 6 of 6 entries
Annotation IDQualifierOMP IDOMP term nameRelative phenotype informationExperimental conditionECO IDECO term nameReferenceAnnotation ExtensionNotes

OMP_AN:31692

OMP:0006044

decreased resistance to beta-lactam

Relative to: OMP_AN:27530


Genotype differences:

+lacZ4787(del)( +cysM750(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Amoxicillin
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:2676) Amoxicillin

  • chemical concentration= 0.25 ug/ml
  • normalized fitness= -3.55

OMP_AN:31693

OMP:0007879

decreased resistance to an antimicrobial agent

Relative to: OMP_AN:27549


Genotype differences:

+lacZ4787(del)( +cysM750(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:CHIR-090
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:134107 CID:11546620) CHIR-090

  • chemical concentration= 0.05 ug/ml
  • normalized fitness= -3.43

OMP_AN:31694

OMP:0007074

increased growth factor requirement

Relative to: OMP_AN:27604


Genotype differences:

+lacZ4787(del)( +cysM750(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:M9
  • medium:Glycerol
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:17754) Glycerol

  • chemical concentration= 0.4%
  • normalized fitness= 3.55

OMP_AN:31695

OMP:0007071

increased organic carbon source utilization

Relative to: OMP_AN:27614


Genotype differences:

+lacZ4787(del)( +cysM750(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:M9
  • medium:Glycerol
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:17754) Glycerol

  • chemical concentration= 0.4%
  • normalized fitness= 3.55

OMP_AN:31696

OMP:0007250

increased resistance to a chemical

Relative to: OMP_AN:27512


Genotype differences:

+lacZ4787(del)( +cysM750(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Hydroxyurea
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:44423) Hydroxyurea

  • chemical concentration= 10 mM
  • normalized fitness= 3.72

OMP_AN:31697

OMP:0005098

decreased resistance to UV radiation 

Relative to: OMP_AN:27619


Genotype differences:

+lacZ4787(del)( +cysM750(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:UV
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

  • chemical concentration= 18 sec
  • normalized fitness= -4.16
Showing 1 to 6 of 6 entries


Additional Information

Links

References

See Help:References for how to manage references in OMPwiki.

  1. Jump up Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed OMPwiki page
  2. Jump up to: 2.0 2.1 2.2 2.3 2.4 2.5 Nichols, RJ et al. (2011) Phenotypic landscape of a bacterial cell. Cell 144 143-56 PubMed OMPwiki page