OMP ST:72768 ! Escherichia coli K-12 JW2374

From OMPwiki

Strain Summary

Strain Name

Escherichia coli K-12 JW2374

Synonyms

JW2374-1

Taxon Information
  • Pangenome: Escherichia coli
  • Subspecies and/or strain: K-12
  • NCBI Taxonomy ID: 83333
Genotype

DE(araD-araB)567 DE(rhaD-rhaB)568 F- Lambda- hsdR514 lacZ4787(del)(::rrnB-3) rph-1 yfdY786(del)::FRT-kan-FRT

Strain Reference
Strain availability

Coli Genetic Stock Center

Ancestry

parent:OMP ST:800 ! Escherichia coli K-12 BW25113

Annotated phenotypes



Notes

Phenotype Annotation

Annotation ID Qualifier OMP ID OMP term name Relative phenotype information Experimental condition ECO ID ECO term name Reference Annotation Extension Notes

OMP_AN:31598

OMP:0007878

increased resistance to an antimicrobial agent

Relative to: OMP_AN:27556


Genotype differences:

+lacZ4787(del)( +yfdY786(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Bicyclomycin
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:60584) Bicyclomycin

  • chemical concentration= 10 ug/ml
  • normalized fitness= 5.4

OMP_AN:31599

OMP:0007903

decreased resistance to bile acids

Relative to: OMP_AN:27587


Genotype differences:

+lacZ4787(del)( +yfdY786(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Bile salts chemical-Mixture of sodium cholate (CHEBI:26711) and sodium deoxycholate (CHEBI:9177)
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:26711 + CHEBI:9177) Bile salts chemical-Mixture of sodium cholate (CHEBI:26711) and sodium deoxycholate (CHEBI:9177)

  • chemical concentration= 2 %
  • normalized fitness= -6.93

OMP_AN:31600

OMP:0007250

increased resistance to a chemical

Relative to: OMP_AN:27514


Genotype differences:

+lacZ4787(del)( +yfdY786(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Chlorpromazine hydrochloride
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:3649) Chlorpromazine hydrochloride

  • chemical concentration= 3 uM
  • normalized fitness= 3.78

OMP_AN:31601

OMP:0007903

decreased resistance to bile acids

Relative to: OMP_AN:27585


Genotype differences:

+lacZ4787(del)( +yfdY786(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Cholate sodium hydrate
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CID:23679061) Cholate sodium hydrate

  • chemical concentration= 2 %
  • normalized fitness= -3.63

OMP_AN:31602

OMP:0007913

increased resistance to a chelator

Relative to: OMP_AN:27507


Genotype differences:

+lacZ4787(del)( +yfdY786(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:EDTA disodium salt dihydrate
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:64758) EDTA disodium salt dihydrate

  • chemical concentration= 0.5 mM
  • normalized fitness= 3.56

OMP_AN:31603

OMP:0007074

increased growth factor requirement

Relative to: OMP_AN:27600


Genotype differences:

+lacZ4787(del)( +yfdY786(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:MOPS
  • medium:NH4Cl
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:31206) NH4Cl

  • chemical concentration= 9.5mm
  • normalized fitness= 2.91

OMP_AN:31604

OMP:0007033

increased utilization of inorganic nitrogen source

Relative to: OMP_AN:27610


Genotype differences:

+lacZ4787(del)( +yfdY786(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:MOPS
  • medium:NH4Cl
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:31206) NH4Cl

  • chemical concentration= 9.5mm
  • normalized fitness= 2.91

OMP_AN:31605

OMP:0007250

increased resistance to a chemical

Relative to: OMP_AN:27574


Genotype differences:

+lacZ4787(del)( +yfdY786(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Paraquat dichloride
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:28786) Paraquat dichloride

  • chemical concentration= 18 uM
  • normalized fitness= 4.31

OMP_AN:31606

OMP:0007250

increased resistance to a chemical

Relative to: OMP_AN:27573


Genotype differences:

+lacZ4787(del)( +yfdY786(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Procaine hydrochloride
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:8431) Procaine hydrochloride

  • chemical concentration= 5 mM
  • normalized fitness= 4.18

OMP_AN:31607

OMP:0007879

decreased resistance to an antimicrobial agent

Relative to: OMP_AN:27544


Genotype differences:

+lacZ4787(del)( +yfdY786(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Chloramphenicol
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:17698) Chloramphenicol

  • chemical concentration= 0.5 ug/ml
  • normalized fitness= -3.82

OMP_AN:31608

OMP:0006063

increased resistance to tetracycline

Relative to: OMP_AN:27522


Genotype differences:

+lacZ4787(del)( +yfdY786(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Doxycycline hyclate
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:34730) Doxycycline hyclate

  • chemical concentration= 0.75 ug/ml
  • normalized fitness= 3.44

OMP_AN:31609

OMP:0007521

decreased population growth in high osmolarity

Relative to: OMP_AN:27595


Genotype differences:

+lacZ4787(del)( +yfdY786(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:NaCl
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:26710) NaCl

  • chemical concentration= 150 mM
  • normalized fitness= -4.19

OMP_AN:31610

OMP:0007832

increased resistance to sulfonamide

Relative to: OMP_AN:27576


Genotype differences:

+lacZ4787(del)( +yfdY786(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Sulfamethizole
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:9331) Sulfamethizole

  • chemical concentration= 300 ug/ml
  • normalized fitness= 4.36


Additional Information

Links

References

See Help:References for how to manage references in OMPwiki.

  1. Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed OMPwiki page
  2. 2.00 2.01 2.02 2.03 2.04 2.05 2.06 2.07 2.08 2.09 2.10 2.11 2.12 Nichols, RJ et al. (2011) Phenotypic landscape of a bacterial cell. Cell 144 143-56 PubMed OMPwiki page