OMP ST:72187 ! Escherichia coli K-12 JW1517

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Strain Summary

Strain Name

Escherichia coli K-12 JW1517

Synonyms

JW1517-1

Taxon Information
  • Pangenome: Escherichia coli
  • Subspecies and/or strain: K-12
  • NCBI Taxonomy ID: 83333
Genotype

DE(araD-araB)567 DE(rhaD-rhaB)568 F- Lambda- hsdR514 lacZ4787(del)(::rrnB-3) rph-1 yneH745(del)::FRT-kan-FRT

Strain Reference
Strain availability

Coli Genetic Stock Center

Ancestry

parent:OMP ST:800 ! Escherichia coli K-12 BW25113

Annotated phenotypes


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Notes

Phenotype Annotation

Annotation ID Qualifier OMP ID OMP term name Relative phenotype information Experimental condition ECO ID ECO term name Reference Annotation Extension Notes

OMP_AN:30329

OMP:0007250

increased resistance to a chemical

Relative to: OMP_AN:27575

Genotype differences:

+lacZ4787(del)( +yneH745(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Acriflavine (acriflavinium chloride)
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:74728) Acriflavine (acriflavinium chloride)

  • chemical concentration= 2 ug/ml
  • normalized fitness= 3.3

OMP_AN:30330

OMP:0006044

decreased resistance to beta-lactam

Relative to: OMP_AN:27535

Genotype differences:

+lacZ4787(del)( +yneH745(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Cefsulodin sodium salt hydrate
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:31380) Cefsulodin sodium salt hydrate

  • chemical concentration= 12 ug/ml
  • normalized fitness= -3.54

OMP_AN:30331

OMP:0007902

increased resistance to bile acids

Relative to: OMP_AN:27585

Genotype differences:

+lacZ4787(del)( +yneH745(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Cholate sodium hydrate
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CID:23679061) Cholate sodium hydrate

  • chemical concentration= 0.1 %
  • normalized fitness= 6.33

OMP_AN:30332

OMP:0007249

decreased resistance to a chemical

Relative to: OMP_AN:27511

Genotype differences:

+lacZ4787(del)( +yneH745(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:(-)-Epinephrine
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:28918) (-)-Epinephrine

  • chemical concentration= 50 uM
  • normalized fitness= -4.33

OMP_AN:30333

OMP:0007878

increased resistance to an antimicrobial agent

Relative to: OMP_AN:27553

Genotype differences:

+lacZ4787(del)( +yneH745(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Puromycin dihydrochloride
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:8642) Puromycin dihydrochloride

  • chemical concentration= 25 ug/ml
  • normalized fitness= 4.78

OMP_AN:30334

OMP:0007878

increased resistance to an antimicrobial agent

Relative to: OMP_AN:27566

Genotype differences:

+lacZ4787(del)( +yneH745(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Vancomycin HCl hydrate
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:9932) Vancomycin HCl hydrate

  • chemical concentration= 50 ug/ml
  • normalized fitness= 3.38

OMP_AN:30335

OMP:0007878

increased resistance to an antimicrobial agent

Relative to: OMP_AN:27544

Genotype differences:

+lacZ4787(del)( +yneH745(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Chloramphenicol
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:17698) Chloramphenicol

  • chemical concentration= 0.5 ug/ml
  • normalized fitness= 4.66

OMP_AN:30336

OMP:0007912

decreased resistance to a chelator

Relative to: OMP_AN:27508

Genotype differences:

+lacZ4787(del)( +yneH745(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:EGTA
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:30740) EGTA

  • chemical concentration= 1 mM
  • normalized fitness= -3.28

OMP_AN:30337

OMP:0007008

decreased population growth at acidic pH

Relative to: OMP_AN:27622

Genotype differences:

+lacZ4787(del)( +yneH745(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:MES-HOMOPIPES
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (**SID:329817955 SID:57651422) MES-HOMOPIPES

  • chemical concentration= 6
  • normalized fitness= -3.3
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Additional Information

Links

References

See Help:References for how to manage references in OMPwiki.

  1. ↑ Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed OMPwiki page
  2. ↑ 2.0 2.1 2.2 2.3 2.4 2.5 2.6 2.7 2.8 Nichols, RJ et al. (2011) Phenotypic landscape of a bacterial cell. Cell 144 143-56 PubMed OMPwiki page
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