OMP ST:72012 ! Escherichia coli K-12 JW1256

From OMPwiki

Strain Summary

Strain Name

Escherichia coli K-12 JW1256

Synonyms

JW1256-1

Taxon Information
  • Pangenome: Escherichia coli
  • Subspecies and/or strain: K-12
  • NCBI Taxonomy ID: 83333
Genotype

DE(araD-araB)567 DE(rhaD-rhaB)568 F- Lambda- hsdR514 lacZ4787(del)(::rrnB-3) rph-1 trpE772(del)::FRT-kan-FRT

Strain Reference
Strain availability

Coli Genetic Stock Center

Ancestry

parent:OMP ST:800 ! Escherichia coli K-12 BW25113

Annotated phenotypes



Notes

Phenotype Annotation

Annotation ID Qualifier OMP ID OMP term name Relative phenotype information Experimental condition ECO ID ECO term name Reference Annotation Extension Notes

OMP_AN:30038

OMP:0007886

decreased resistance to a metal

Relative to: OMP_AN:27579


Genotype differences:

+lacZ4787(del)( +trpE772(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Copper stress-CuCl2 dihydrate
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:86318) Copper stress-CuCl2 dihydrate

  • chemical concentration= 1 mM (LB)
  • normalized fitness= -3.35

OMP_AN:30039

OMP:0007075

decreased growth factor requirement

Relative to: OMP_AN:27603


Genotype differences:

+lacZ4787(del)( +trpE772(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:M9
  • medium:D-Glucose anhydrous
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:42758) D-Glucose anhydrous

  • chemical concentration= 0.2%
  • normalized fitness= -5.24

OMP_AN:30040

OMP:0007070

decreased organic carbon source utilization

Relative to: OMP_AN:27613


Genotype differences:

+lacZ4787(del)( +trpE772(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:M9
  • medium:D-Glucose anhydrous
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:42758) D-Glucose anhydrous

  • chemical concentration= 0.2%
  • normalized fitness= -5.24

OMP_AN:30041

OMP:0007075

decreased growth factor requirement

Relative to: OMP_AN:27598


Genotype differences:

+lacZ4787(del)( +trpE772(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:MOPS
  • medium:Iron excess-FeSO4 heptahydride
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:75836) Iron excess-FeSO4 heptahydride

  • chemical concentration= 1mM (MOPS; normal 100uM)
  • normalized fitness= -4.6

OMP_AN:30042

OMP:0007886

decreased resistance to a metal

Relative to: OMP_AN:27608


Genotype differences:

+lacZ4787(del)( +trpE772(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:MOPS
  • medium:Iron excess-FeSO4 heptahydride
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:75836) Iron excess-FeSO4 heptahydride

  • chemical concentration= 1mM (MOPS; normal 100uM)
  • normalized fitness= -4.6

OMP_AN:30043

OMP:0007075

decreased growth factor requirement

Relative to: OMP_AN:27599


Genotype differences:

+lacZ4787(del)( +trpE772(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:MOPS
  • medium:Iron starvation-FeSO4·7H2O
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:75836) Iron starvation-FeSO4·7H2O

  • chemical concentration= 2uM (MOPS; normal 100uM)
  • normalized fitness= -4

OMP_AN:30044

OMP:0007937

decreased resistance to nutrient limitation

Relative to: OMP_AN:27609


Genotype differences:

+lacZ4787(del)( +trpE772(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:MOPS
  • medium:Iron starvation-FeSO4·7H2O
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:75836) Iron starvation-FeSO4·7H2O

  • chemical concentration= 2uM (MOPS; normal 100uM)
  • normalized fitness= -4


Additional Information

Links

References

See Help:References for how to manage references in OMPwiki.

  1. Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed OMPwiki page
  2. 2.0 2.1 2.2 2.3 2.4 2.5 2.6 Nichols, RJ et al. (2011) Phenotypic landscape of a bacterial cell. Cell 144 143-56 PubMed OMPwiki page