OMP ST:71988 ! Escherichia coli K-12 JW1221

From OMPwiki

Strain Summary

Strain Name

Escherichia coli K-12 JW1221

Synonyms

JW1221-3

Taxon Information
  • Pangenome: Escherichia coli
  • Subspecies and/or strain: K-12
  • NCBI Taxonomy ID: 83333
Genotype

DE(araD-araB)567 DE(rhaD-rhaB)568 F- Lambda- hsdR514 lacZ4787(del)(::rrnB-3) rph-1 ychJ742(del)::FRT-kan-FRT

Strain Reference
Strain availability

Coli Genetic Stock Center

Ancestry

parent:OMP ST:800 ! Escherichia coli K-12 BW25113

Annotated phenotypes


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Notes

Phenotype Annotation

Annotation ID Qualifier OMP ID OMP term name Relative phenotype information Experimental condition ECO ID ECO term name Reference Annotation Extension Notes

OMP_AN:29905

OMP:0007878

increased resistance to an antimicrobial agent

Relative to: OMP_AN:27557

Genotype differences:

+lacZ4787(del)( +ychJ742(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:A22
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CID:2830968) A22

  • chemical concentration= 0.5 ug/ml
  • normalized fitness= 3.89

OMP_AN:29906

OMP:0007902

increased resistance to bile acids

Relative to: OMP_AN:27585

Genotype differences:

+lacZ4787(del)( +ychJ742(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Cholate sodium hydrate
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CID:23679061) Cholate sodium hydrate

  • chemical concentration= 0.1 %
  • normalized fitness= 4.17

OMP_AN:29907

OMP:0006036

decreased resistance to macrolide

Relative to: OMP_AN:27519

Genotype differences:

+lacZ4787(del)( +ychJ742(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Clarithromycin
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:3732) Clarithromycin

  • chemical concentration= 5 ug/ml
  • normalized fitness= -3.34

OMP_AN:29908

OMP:0007074

increased growth factor requirement

Relative to: OMP_AN:27606

Genotype differences:

+lacZ4787(del)( +ychJ742(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:M9
  • medium:N-Acetyl-D-glucosamine
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:506227) N-Acetyl-D-glucosamine

  • chemical concentration= 0.15%
  • normalized fitness= 3.35

OMP_AN:29909

OMP:0007071

increased organic carbon source utilization

Relative to: OMP_AN:27616

Genotype differences:

+lacZ4787(del)( +ychJ742(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:M9
  • medium:N-Acetyl-D-glucosamine
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:506227) N-Acetyl-D-glucosamine

  • chemical concentration= 0.15%
  • normalized fitness= 3.35

OMP_AN:29910

OMP:0007249

decreased resistance to a chemical

Relative to: OMP_AN:27512

Genotype differences:

+lacZ4787(del)( +ychJ742(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Hydroxyurea
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:44423) Hydroxyurea

  • chemical concentration= 10 mM
  • normalized fitness= -4.84

OMP_AN:29911

OMP:0007074

increased growth factor requirement

Relative to: OMP_AN:27605

Genotype differences:

+lacZ4787(del)( +ychJ742(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:M9
  • medium:D-(+) Maltose monohydrate
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CID:23615261) D-(+) Maltose monohydrate

  • chemical concentration= 0.1%
  • normalized fitness= 2.98

OMP_AN:29912

OMP:0007071

increased organic carbon source utilization

Relative to: OMP_AN:27615

Genotype differences:

+lacZ4787(del)( +ychJ742(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:M9
  • medium:D-(+) Maltose monohydrate
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CID:23615261) D-(+) Maltose monohydrate

  • chemical concentration= 0.1%
  • normalized fitness= 2.98

OMP_AN:29913

OMP:0007878

increased resistance to an antimicrobial agent

Relative to: OMP_AN:27503

Genotype differences:

+lacZ4787(del)( +ychJ742(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Nitrofurantoin
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:71415) Nitrofurantoin

  • chemical concentration= 1.5 ug/ml
  • normalized fitness= 3.33

OMP_AN:29914

OMP:0007878

increased resistance to an antimicrobial agent

Relative to: OMP_AN:27547

Genotype differences:

+lacZ4787(del)( +ychJ742(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Hydrogen peroxide
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:16240) Hydrogen peroxide

  • chemical concentration= 0.5 mM
  • normalized fitness= 4.09

OMP_AN:29915

OMP:0007902

increased resistance to bile acids

Relative to: OMP_AN:27584

Genotype differences:

+lacZ4787(del)( +ychJ742(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Taurocholic acid sodium salt hydrate
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CID:23687511) Taurocholic acid sodium salt hydrate

  • chemical concentration= 0.1 %
  • normalized fitness= 3.98

OMP_AN:29916

OMP:0006044

decreased resistance to beta-lactam

Relative to: OMP_AN:27536

Genotype differences:

+lacZ4787(del)( +ychJ742(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Ceftazidime hydrate
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:3509) Ceftazidime hydrate

  • chemical concentration= 0.075 ug/ml
  • normalized fitness= -8.21

OMP_AN:29917

OMP:0006040

decreased resistance to quinolone

Relative to: OMP_AN:27541

Genotype differences:

+lacZ4787(del)( +ychJ742(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Nalidixic acid sodium salt
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CID:3864541) Nalidixic acid sodium salt

  • chemical concentration= 2 ug/ml
  • normalized fitness= -4.88

OMP_AN:29918

OMP:0007878

increased resistance to an antimicrobial agent

Relative to: OMP_AN:27551

Genotype differences:

+lacZ4787(del)( +ychJ742(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Rifampicin
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:28077) Rifampicin

  • chemical concentration= 2 ug/ml
  • normalized fitness= 3.93

OMP_AN:29919

OMP:0006036

decreased resistance to macrolide

Relative to: OMP_AN:27521

Genotype differences:

+lacZ4787(del)( +ychJ742(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Spiramycin
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CID:6419898) Spiramycin

  • chemical concentration= 5 ug/ml
  • normalized fitness= -3.97
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Additional Information

Links

References

See Help:References for how to manage references in OMPwiki.

  1. ↑ Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed OMPwiki page
  2. ↑ 2.00 2.01 2.02 2.03 2.04 2.05 2.06 2.07 2.08 2.09 2.10 2.11 2.12 2.13 2.14 Nichols, RJ et al. (2011) Phenotypic landscape of a bacterial cell. Cell 144 143-56 PubMed OMPwiki page
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