OMP ST:71545 ! Escherichia coli K-12 JW0512

Strain Summary

Strain Name	Escherichia coli K-12 JW0512
Synonyms	JW0512-1
Taxon Information	Pangenome: Escherichia coli Subspecies and/or strain: K-12 NCBI Taxonomy ID: 83333
Genotype	DE(araD-araB)567 DE(rhaD-rhaB)568 F- Lambda- hsdR514 lacZ4787(del)(::rrnB-3) rph-1 purE736(del)::FRT-kan-FRT
Strain Reference	PMID:16738554^[1] Coli Genetic Stock Center
Strain availability	Coli Genetic Stock Center
Ancestry	parent:OMP ST:800 ! Escherichia coli K-12 BW25113
Annotated phenotypes
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Notes

Phenotype Annotation

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Annotation ID	OMP ID	OMP term name	Relative phenotype information	Experimental condition	ECO ID	ECO term name	Reference	Annotation Extension	Notes
OMP_AN:28624	OMP:0007075	decreased growth factor requirement	Relative to: OMP_AN:27601 Genotype differences: +lacZ4787(del)( +purE736(del) -lacZ4787(del) Condition differences: no differences	temperature:37C medium:M9 medium:Sodium Acetate anhydrous	ECO:0007005	high throughput direct assay evidence used in manual assertion	PMID:21185072^[2]	towards some (CHEBI:32954) Sodium Acetate anhydrous	chemical concentration= 0.6% normalized fitness= -15.47
OMP_AN:28625	OMP:0007070	decreased organic carbon source utilization	Relative to: OMP_AN:27611 Genotype differences: +lacZ4787(del)( +purE736(del) -lacZ4787(del) Condition differences: no differences	temperature:37C medium:M9 medium:Sodium Acetate anhydrous	ECO:0007005	high throughput direct assay evidence used in manual assertion	PMID:21185072^[2]	towards some (CHEBI:32954) Sodium Acetate anhydrous	chemical concentration= 0.6% normalized fitness= -15.47
OMP_AN:28626	OMP:0007903	decreased resistance to bile acids	Relative to: OMP_AN:27587 Genotype differences: +lacZ4787(del)( +purE736(del) -lacZ4787(del) Condition differences: no differences	temperature:37C medium:LB agar medium:Bile salts chemical-Mixture of sodium cholate (CHEBI:26711) and sodium deoxycholate (CHEBI:9177)	ECO:0007005	high throughput direct assay evidence used in manual assertion	PMID:21185072^[2]	towards some (CHEBI:26711 + CHEBI:9177) Bile salts chemical-Mixture of sodium cholate (CHEBI:26711) and sodium deoxycholate (CHEBI:9177)	chemical concentration= 0.1 % normalized fitness= -7.2
OMP_AN:28627	OMP:0007902	increased resistance to bile acids	Relative to: OMP_AN:27587 Genotype differences: +lacZ4787(del)( +purE736(del) -lacZ4787(del) Condition differences: no differences	temperature:37C medium:LB agar medium:Bile salts chemical-Mixture of sodium cholate (CHEBI:26711) and sodium deoxycholate (CHEBI:9177)	ECO:0007005	high throughput direct assay evidence used in manual assertion	PMID:21185072^[2]	towards some (CHEBI:26711 + CHEBI:9177) Bile salts chemical-Mixture of sodium cholate (CHEBI:26711) and sodium deoxycholate (CHEBI:9177)	chemical concentration= 1 % normalized fitness= 3.86
OMP_AN:28628	OMP:0006036	decreased resistance to macrolide	Relative to: OMP_AN:27519 Genotype differences: +lacZ4787(del)( +purE736(del) -lacZ4787(del) Condition differences: no differences	temperature:37C medium:LB agar medium:Clarithromycin	ECO:0007005	high throughput direct assay evidence used in manual assertion	PMID:21185072^[2]	towards some (CHEBI:3732) Clarithromycin	chemical concentration= 1 ug/ml normalized fitness= -4.45
OMP_AN:28629	OMP:0007903	decreased resistance to bile acids	Relative to: OMP_AN:27586 Genotype differences: +lacZ4787(del)( +purE736(del) -lacZ4787(del) Condition differences: no differences	temperature:37C medium:LB agar medium:Deoxycholate sodium	ECO:0007005	high throughput direct assay evidence used in manual assertion	PMID:21185072^[2]	towards some (CHEBI:9177) Deoxycholate sodium	chemical concentration= 2 % normalized fitness= -8.28
OMP_AN:28630	OMP:0007075	decreased growth factor requirement	Relative to: OMP_AN:27606 Genotype differences: +lacZ4787(del)( +purE736(del) -lacZ4787(del) Condition differences: no differences	temperature:37C medium:M9 medium:N-Acetyl-D-glucosamine	ECO:0007005	high throughput direct assay evidence used in manual assertion	PMID:21185072^[2]	towards some (CHEBI:506227) N-Acetyl-D-glucosamine	chemical concentration= 0.15% normalized fitness= -9.87
OMP_AN:28631	OMP:0007070	decreased organic carbon source utilization	Relative to: OMP_AN:27616 Genotype differences: +lacZ4787(del)( +purE736(del) -lacZ4787(del) Condition differences: no differences	temperature:37C medium:M9 medium:N-Acetyl-D-glucosamine	ECO:0007005	high throughput direct assay evidence used in manual assertion	PMID:21185072^[2]	towards some (CHEBI:506227) N-Acetyl-D-glucosamine	chemical concentration= 0.15% normalized fitness= -9.87
OMP_AN:28632	OMP:0007075	decreased growth factor requirement	Relative to: OMP_AN:27602 Genotype differences: +lacZ4787(del)( +purE736(del) -lacZ4787(del) Condition differences: no differences	temperature:37C medium:M9 medium:D-(+) Glucosamine hydrochloride	ECO:0007005	high throughput direct assay evidence used in manual assertion	PMID:21185072^[2]	towards some (CID:91431) D-(+) Glucosamine hydrochloride	chemical concentration= 0.2% normalized fitness= -13.5
OMP_AN:28633	OMP:0007070	decreased organic carbon source utilization	Relative to: OMP_AN:27612 Genotype differences: +lacZ4787(del)( +purE736(del) -lacZ4787(del) Condition differences: no differences	temperature:37C medium:M9 medium:D-(+) Glucosamine hydrochloride	ECO:0007005	high throughput direct assay evidence used in manual assertion	PMID:21185072^[2]	towards some (CID:91431) D-(+) Glucosamine hydrochloride	chemical concentration= 0.2% normalized fitness= -13.5

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Jump up ↑ Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed OMPwiki page
↑ ^{Jump up to: 2.00} ^2.01 ^2.02 ^2.03 ^2.04 ^2.05 ^2.06 ^2.07 ^2.08 ^2.09 ^2.10 ^2.11 ^2.12 ^2.13 ^2.14 ^2.15 ^2.16 ^2.17 ^2.18 ^2.19 ^2.20 ^2.21 ^2.22 ^2.23 ^2.24 ^2.25 ^2.26 Nichols, RJ et al. (2011) Phenotypic landscape of a bacterial cell. Cell 144 143-56 PubMed OMPwiki page

[PMID:16738554-1] Jump up ↑ Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed OMPwiki page

[PMID:21185072-2] {Jump up to: 2.00} ^2.01 ^2.02 ^2.03 ^2.04 ^2.05 ^2.06 ^2.07 ^2.08 ^2.09 ^2.10 ^2.11 ^2.12 ^2.13 ^2.14 ^2.15 ^2.16 ^2.17 ^2.18 ^2.19 ^2.20 ^2.21 ^2.22 ^2.23 ^2.24 ^2.25 ^2.26 Nichols, RJ et al. (2011) Phenotypic landscape of a bacterial cell. Cell 144 143-56 PubMed OMPwiki page

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OMP ST:71545 ! Escherichia coli K-12 JW0512

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Strain Summary

Notes

Phenotype Annotation

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