OMP ST:71511 ! Escherichia coli K-12 JW0468

From OMPwiki

Strain Summary

Strain Name

Escherichia coli K-12 JW0468

Synonyms

JW0468-1

Taxon Information
  • Pangenome: Escherichia coli
  • Subspecies and/or strain: K-12
  • NCBI Taxonomy ID: 83333
Genotype

DE(araD-araB)567 DE(rhaD-rhaB)568 F- Lambda- hsdR514 lacZ4787(del)(::rrnB-3) rph-1 fsr-761(del)::FRT-kan-FRT

Strain Reference
Strain availability

Coli Genetic Stock Center

Ancestry

parent:OMP ST:800 ! Escherichia coli K-12 BW25113

Annotated phenotypes



Notes

Phenotype Annotation

Annotation ID Qualifier OMP ID OMP term name Relative phenotype information Experimental condition ECO ID ECO term name Reference Annotation Extension Notes

OMP_AN:28559

OMP:0007879

decreased resistance to an antimicrobial agent

Relative to: OMP_AN:27557


Genotype differences:

+lacZ4787(del)( +fsr-761(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:A22
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CID:2830968) A22

  • chemical concentration= 5 ug/ml
  • normalized fitness= -3.4

OMP_AN:28560

OMP:0007074

increased growth factor requirement

Relative to: OMP_AN:27603


Genotype differences:

+lacZ4787(del)( +fsr-761(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:M9
  • medium:D-Glucose anhydrous
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:42758) D-Glucose anhydrous

  • chemical concentration= 0.2%
  • normalized fitness= 2.95

OMP_AN:28561

OMP:0007071

increased organic carbon source utilization

Relative to: OMP_AN:27613


Genotype differences:

+lacZ4787(del)( +fsr-761(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:M9
  • medium:D-Glucose anhydrous
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:42758) D-Glucose anhydrous

  • chemical concentration= 0.2%
  • normalized fitness= 2.95

OMP_AN:28562

OMP:0007879

decreased resistance to an antimicrobial agent

Relative to: OMP_AN:27545


Genotype differences:

+lacZ4787(del)( +fsr-761(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Isoniazid
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:6030) Isoniazid

  • chemical concentration= 1 mM
  • normalized fitness= -3.53

OMP_AN:28563

OMP:0007249

decreased resistance to a chemical

Relative to: OMP_AN:27513


Genotype differences:

+lacZ4787(del)( +fsr-761(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Phenazine methosulfate (PMS)
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:8055) Phenazine methosulfate (PMS)

  • chemical concentration= 0.1 mM
  • normalized fitness= -3.71

OMP_AN:28564

OMP:0006040

decreased resistance to quinolone

Relative to: OMP_AN:27541


Genotype differences:

+lacZ4787(del)( +fsr-761(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Nalidixic acid sodium salt
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CID:3864541) Nalidixic acid sodium salt

  • chemical concentration= 1.5 ug/ml
  • normalized fitness= -3.31

OMP_AN:28565

OMP:0006040

decreased resistance to quinolone

Relative to: OMP_AN:27542


Genotype differences:

+lacZ4787(del)( +fsr-761(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Norfloxacin
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:100246) Norfloxacin

  • chemical concentration= 0.02 ug/ml
  • normalized fitness= -4.69


Additional Information

Links

References

See Help:References for how to manage references in OMPwiki.

  1. Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed OMPwiki page
  2. 2.0 2.1 2.2 2.3 2.4 2.5 2.6 Nichols, RJ et al. (2011) Phenotypic landscape of a bacterial cell. Cell 144 143-56 PubMed OMPwiki page