OMP ST:71189 ! Escherichia coli K-12 GSO214

From OMPwiki

Strain Summary

Strain Name

Escherichia coli K-12 GSO214

Synonyms
Taxon Information
  • Pangenome: Escherichia coli
  • Subspecies and/or strain: K-12
  • NCBI Taxonomy ID: 83333
Genotype

F- Lambda- rph-1 ybhU(del)::FRT-kan-FRT

Strain Reference

PMID:19734312[1]

Strain availability
Ancestry

parent:OMP ST:159 ! Escherichia coli K-12 MG1655

Annotated phenotypes


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Notes

Phenotype Annotation

Annotation ID Qualifier OMP ID OMP term name Relative phenotype information Experimental condition ECO ID ECO term name Reference Annotation Extension Notes

OMP_AN:36959

OMP:0007902

increased resistance to bile acids

Relative to: OMP_AN:27587

Genotype differences:

+ybhU(del) -DE(araD-araB)567 -DE(rhaD-rhaB)568 -hsdR514 -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Bile salts chemical-Mixture of sodium cholate (CHEBI:26711) and sodium deoxycholate (CHEBI:9177)
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:26711 + CHEBI:9177) Bile salts chemical-Mixture of sodium cholate (CHEBI:26711) and sodium deoxycholate (CHEBI:9177)

  • chemical concentration= 2 %
  • normalized fitness= 3.19

OMP_AN:36960

OMP:0007879

decreased resistance to an antimicrobial agent

Relative to: OMP_AN:27561

Genotype differences:

+ybhU(del) -DE(araD-araB)567 -DE(rhaD-rhaB)568 -hsdR514 -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Bleomycin sulfate
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:34582) Bleomycin sulfate

  • chemical concentration= 0.5 ug/ml
  • normalized fitness= -4.56

OMP_AN:36961

OMP:0006045

increased resistance to beta-lactam compound

Relative to: OMP_AN:27534

Genotype differences:

+ybhU(del) -DE(araD-araB)567 -DE(rhaD-rhaB)568 -hsdR514 -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Cefoxitin sodium hydrate
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:3501) Cefoxitin sodium hydrate

  • chemical concentration= 0.5 ug/ml
  • normalized fitness= 3.47

OMP_AN:36962

OMP:0007878

increased resistance to an antimicrobial agent

Relative to: OMP_AN:27548

Genotype differences:

+ybhU(del) -DE(araD-araB)567 -DE(rhaD-rhaB)568 -hsdR514 -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Cerulenin
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:171741) Cerulenin

  • chemical concentration= 4 ug/ml
  • normalized fitness= 4.35

OMP_AN:36963

OMP:0007250

increased resistance to a chemical

Relative to: OMP_AN:27509

Genotype differences:

+ybhU(del) -DE(araD-araB)567 -DE(rhaD-rhaB)568 -hsdR514 -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Cisplatin-cis-Diamineplatinum(II) dichloride
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:27899) Cisplatin-cis-Diamineplatinum(II) dichloride

  • chemical concentration= 100 ug/ml
  • normalized fitness= 5.22

OMP_AN:36964

OMP:0006037

increased resistance to macrolide

Relative to: OMP_AN:27519

Genotype differences:

+ybhU(del) -DE(araD-araB)567 -DE(rhaD-rhaB)568 -hsdR514 -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Clarithromycin
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:3732) Clarithromycin

  • chemical concentration= 1 ug/ml
  • normalized fitness= 3.68

OMP_AN:36965

OMP:0007250

increased resistance to a chemical

Relative to: OMP_AN:27501

Genotype differences:

+ybhU(del) -DE(araD-araB)567 -DE(rhaD-rhaB)568 -hsdR514 -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Dibucaine
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:247956) Dibucaine

  • chemical concentration= 0.8 mM
  • normalized fitness= 4.6

OMP_AN:36966

OMP:0007250

increased resistance to a chemical

Relative to: OMP_AN:27572

Genotype differences:

+ybhU(del) -DE(araD-araB)567 -DE(rhaD-rhaB)568 -hsdR514 -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Epigallocatechin gallate (EGCG)
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:4806) Epigallocatechin gallate (EGCG)

  • chemical concentration= 5 uM
  • normalized fitness= 4.57

OMP_AN:36967

OMP:0007878

increased resistance to an antimicrobial agent

Relative to: OMP_AN:27515

Genotype differences:

+ybhU(del) -DE(araD-araB)567 -DE(rhaD-rhaB)568 -hsdR514 -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Fusidic acid sodium salt
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CID:126963539) Fusidic acid sodium salt

  • chemical concentration= 5 ug/ml
  • normalized fitness= 3.76

OMP_AN:36968

OMP:0007075

decreased growth factor requirement

Relative to: OMP_AN:27603

Genotype differences:

+ybhU(del) -DE(araD-araB)567 -DE(rhaD-rhaB)568 -hsdR514 -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:M9
  • medium:D-Glucose anhydrous
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:42758) D-Glucose anhydrous

  • chemical concentration= 0.2%
  • normalized fitness= -2.99

OMP_AN:36969

OMP:0007070

decreased organic carbon source utilization

Relative to: OMP_AN:27613

Genotype differences:

+ybhU(del) -DE(araD-araB)567 -DE(rhaD-rhaB)568 -hsdR514 -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:M9
  • medium:D-Glucose anhydrous
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:42758) D-Glucose anhydrous

  • chemical concentration= 0.2%
  • normalized fitness= -2.99

OMP_AN:36970

OMP:0007250

increased resistance to a chemical

Relative to: OMP_AN:27512

Genotype differences:

+ybhU(del) -DE(araD-araB)567 -DE(rhaD-rhaB)568 -hsdR514 -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Hydroxyurea
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:44423) Hydroxyurea

  • chemical concentration= 10 mM
  • normalized fitness= 4.45

OMP_AN:36971

OMP:0007075

decreased growth factor requirement

Relative to: OMP_AN:27605

Genotype differences:

+ybhU(del) -DE(araD-araB)567 -DE(rhaD-rhaB)568 -hsdR514 -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:M9
  • medium:D-(+) Maltose monohydrate
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CID:23615261) D-(+) Maltose monohydrate

  • chemical concentration= 0.1%
  • normalized fitness= -3.48

OMP_AN:36972

OMP:0007070

decreased organic carbon source utilization

Relative to: OMP_AN:27615

Genotype differences:

+ybhU(del) -DE(araD-araB)567 -DE(rhaD-rhaB)568 -hsdR514 -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:M9
  • medium:D-(+) Maltose monohydrate
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CID:23615261) D-(+) Maltose monohydrate

  • chemical concentration= 0.1%
  • normalized fitness= -3.48

OMP_AN:36973

OMP:0007249

decreased resistance to a chemical

Relative to: OMP_AN:27510

Genotype differences:

+ybhU(del) -DE(araD-araB)567 -DE(rhaD-rhaB)568 -hsdR514 -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Mitomycin C
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:27504) Mitomycin C

  • chemical concentration= 0.1 ug/ml
  • normalized fitness= -5.53

OMP_AN:36974

OMP:0007075

decreased growth factor requirement

Relative to: OMP_AN:27600

Genotype differences:

+ybhU(del) -DE(araD-araB)567 -DE(rhaD-rhaB)568 -hsdR514 -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:MOPS
  • medium:NH4Cl
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:31206) NH4Cl

  • chemical concentration= 9.5mm
  • normalized fitness= -3.48

OMP_AN:36975

OMP:0007032

decreased utilization of inorganic nitrogen source

Relative to: OMP_AN:27610

Genotype differences:

+ybhU(del) -DE(araD-araB)567 -DE(rhaD-rhaB)568 -hsdR514 -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:MOPS
  • medium:NH4Cl
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:31206) NH4Cl

  • chemical concentration= 9.5mm
  • normalized fitness= -3.48

OMP_AN:36976

OMP:0007250

increased resistance to a chemical

Relative to: OMP_AN:27574

Genotype differences:

+ybhU(del) -DE(araD-araB)567 -DE(rhaD-rhaB)568 -hsdR514 -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Paraquat dichloride
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:28786) Paraquat dichloride

  • chemical concentration= 10 uM
  • normalized fitness= 3.52

OMP_AN:36977

OMP:0007878

increased resistance to an antimicrobial agent

Relative to: OMP_AN:27588

Genotype differences:

+ybhU(del) -DE(araD-araB)567 -DE(rhaD-rhaB)568 -hsdR514 -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Triclosan/Irgasan
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:164200) Triclosan/Irgasan

  • chemical concentration= 0.05 ug/ml
  • normalized fitness= 6.64

OMP_AN:36978

OMP:0007895

increased resistance to a surfactant

Relative to: OMP_AN:27583

Genotype differences:

+ybhU(del) -DE(araD-araB)567 -DE(rhaD-rhaB)568 -hsdR514 -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Triton X-100
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:9750) Triton X-100

  • chemical concentration= 0.2 %
  • normalized fitness= 3.82

OMP_AN:36979

OMP:0007878

increased resistance to an antimicrobial agent

Relative to: OMP_AN:27566

Genotype differences:

+ybhU(del) -DE(araD-araB)567 -DE(rhaD-rhaB)568 -hsdR514 -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Vancomycin HCl hydrate
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:9932) Vancomycin HCl hydrate

  • chemical concentration= 20 ug/ml
  • normalized fitness= 4.65

OMP_AN:36980

OMP:0007250

increased resistance to a chemical

Relative to: OMP_AN:27568

Genotype differences:

+ybhU(del) -DE(araD-araB)567 -DE(rhaD-rhaB)568 -hsdR514 -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:(±)-Verapamil hydrochloride
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:53188) (±)-Verapamil hydrochloride

  • chemical concentration= 0.5 mM
  • normalized fitness= 5.86

OMP_AN:36981

OMP:0007878

increased resistance to an antimicrobial agent

Relative to: OMP_AN:27560

Genotype differences:

+ybhU(del) -DE(araD-araB)567 -DE(rhaD-rhaB)568 -hsdR514 -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Bacitracin Zinc Sulfate
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CID:16219032) Bacitracin Zinc Sulfate

  • chemical concentration= 200 ug/ml
  • normalized fitness= 3.56

OMP_AN:36982

OMP:0007879

decreased resistance to an antimicrobial agent

Relative to: OMP_AN:27544

Genotype differences:

+ybhU(del) -DE(araD-araB)567 -DE(rhaD-rhaB)568 -hsdR514 -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Chloramphenicol
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:17698) Chloramphenicol

  • chemical concentration= 1.5 ug/ml
  • normalized fitness= -3.63

OMP_AN:36983

OMP:0006063

increased resistance to tetracycline

Relative to: OMP_AN:27522

Genotype differences:

+ybhU(del) -DE(araD-araB)567 -DE(rhaD-rhaB)568 -hsdR514 -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Doxycycline hyclate
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:34730) Doxycycline hyclate

  • chemical concentration= 1 ug/ml
  • normalized fitness= 3.16

OMP_AN:36984

OMP:0007249

decreased resistance to a chemical

Relative to: OMP_AN:27569

Genotype differences:

+ybhU(del) -DE(araD-araB)567 -DE(rhaD-rhaB)568 -hsdR514 -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:EtOH
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:16236) EtOH

  • chemical concentration= 4 %
  • normalized fitness= -3.25

OMP_AN:36985

OMP:0007075

decreased growth factor requirement

Relative to: OMP_AN:27598

Genotype differences:

+ybhU(del) -DE(araD-araB)567 -DE(rhaD-rhaB)568 -hsdR514 -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:MOPS
  • medium:Iron excess-FeSO4 heptahydride
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:75836) Iron excess-FeSO4 heptahydride

  • chemical concentration= 1mM (MOPS; normal 100uM)
  • normalized fitness= -3.04

OMP_AN:36986

OMP:0007886

decreased resistance to a metal

Relative to: OMP_AN:27608

Genotype differences:

+ybhU(del) -DE(araD-araB)567 -DE(rhaD-rhaB)568 -hsdR514 -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:MOPS
  • medium:Iron excess-FeSO4 heptahydride
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:75836) Iron excess-FeSO4 heptahydride

  • chemical concentration= 1mM (MOPS; normal 100uM)
  • normalized fitness= -3.04

OMP_AN:36987

OMP:0007878

increased resistance to an antimicrobial agent

Relative to: OMP_AN:27565

Genotype differences:

+ybhU(del) -DE(araD-araB)567 -DE(rhaD-rhaB)568 -hsdR514 -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Polymyxin B sulfate
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:8310) Polymyxin B sulfate

  • chemical concentration= 1 ug/ml
  • normalized fitness= 4.16
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Additional Information

Links

References

See Help:References for how to manage references in OMPwiki.

  1. ↑ Hobbs, EC et al. (2010) Small RNAs and small proteins involved in resistance to cell envelope stress and acid shock in Escherichia coli: analysis of a bar-coded mutant collection. J. Bacteriol. 192 59-67 PubMed OMPwiki page
  2. ↑ 2.00 2.01 2.02 2.03 2.04 2.05 2.06 2.07 2.08 2.09 2.10 2.11 2.12 2.13 2.14 2.15 2.16 2.17 2.18 2.19 2.20 2.21 2.22 2.23 2.24 2.25 2.26 2.27 2.28 Nichols, RJ et al. (2011) Phenotypic landscape of a bacterial cell. Cell 144 143-56 PubMed OMPwiki page
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