OMP ST:71174 ! Escherichia coli K-12 GSO182

From OMPwiki

Strain Summary

Strain Name

Escherichia coli K-12 GSO182

Synonyms
Taxon Information
  • Pangenome: Escherichia coli
  • Subspecies and/or strain: K-12
  • NCBI Taxonomy ID: 83333
Genotype

F- Lambda- rph-1 ryjB(del)::FRT-kan-FRT

Strain Reference

PMID:19734312[1]

Strain availability
Ancestry

parent:OMP ST:159 ! Escherichia coli K-12 MG1655

Annotated phenotypes



Notes

Phenotype Annotation

Annotation ID Qualifier OMP ID OMP term name Relative phenotype information Experimental condition ECO ID ECO term name Reference Annotation Extension Notes

OMP_AN:37035

OMP:0007878

increased resistance to an antimicrobial agent

Relative to: OMP_AN:27549


Genotype differences:

+ryjB(del) -DE(araD-araB)567 -DE(rhaD-rhaB)568 -hsdR514 -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:CHIR-090
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:134107 CID:11546620) CHIR-090

  • chemical concentration= 0.025 ug/ml
  • normalized fitness= 3.88

OMP_AN:37036

OMP:0007250

increased resistance to a chemical

Relative to: OMP_AN:27501


Genotype differences:

+ryjB(del) -DE(araD-araB)567 -DE(rhaD-rhaB)568 -hsdR514 -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Dibucaine
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:247956) Dibucaine

  • chemical concentration= 0.8 mM
  • normalized fitness= 3.8

OMP_AN:37037

OMP:0007250

increased resistance to a chemical

Relative to: OMP_AN:27572


Genotype differences:

+ryjB(del) -DE(araD-araB)567 -DE(rhaD-rhaB)568 -hsdR514 -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Epigallocatechin gallate (EGCG)
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:4806) Epigallocatechin gallate (EGCG)

  • chemical concentration= 5 uM
  • normalized fitness= 4.23

OMP_AN:37038

OMP:0007250

increased resistance to a chemical

Relative to: OMP_AN:27593


Genotype differences:

+ryjB(del) -DE(araD-araB)567 -DE(rhaD-rhaB)568 -hsdR514 -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:(-)-Norepinephrine
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:18357) (-)-Norepinephrine

  • chemical concentration= 100 uM
  • normalized fitness= 3.99

OMP_AN:37039

OMP:0007878

increased resistance to an antimicrobial agent

Relative to: OMP_AN:27566


Genotype differences:

+ryjB(del) -DE(araD-araB)567 -DE(rhaD-rhaB)568 -hsdR514 -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Vancomycin HCl hydrate
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:9932) Vancomycin HCl hydrate

  • chemical concentration= 50 ug/ml
  • normalized fitness= 3.58

OMP_AN:37040

OMP:0006045

increased resistance to beta-lactam compound

Relative to: OMP_AN:27533


Genotype differences:

+ryjB(del) -DE(araD-araB)567 -DE(rhaD-rhaB)568 -hsdR514 -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Carbenicillin disodium
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:34609) Carbenicillin disodium

  • chemical concentration= 0.5 ug/ml
  • normalized fitness= 3.53

OMP_AN:37041

OMP:0006044

decreased resistance to beta-lactam

Relative to: OMP_AN:27596


Genotype differences:

+ryjB(del) -DE(araD-araB)567 -DE(rhaD-rhaB)568 -hsdR514 -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Cefaclor
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:3478) Cefaclor

  • chemical concentration= 3 ug/ml
  • normalized fitness= -5.02

OMP_AN:37042

OMP:0007913

increased resistance to a chelator

Relative to: OMP_AN:27508


Genotype differences:

+ryjB(del) -DE(araD-araB)567 -DE(rhaD-rhaB)568 -hsdR514 -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:EGTA
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:30740) EGTA

  • chemical concentration= 1 mM
  • normalized fitness= 3.43

OMP_AN:37043

OMP:0006037

increased resistance to macrolide

Relative to: OMP_AN:27521


Genotype differences:

+ryjB(del) -DE(araD-araB)567 -DE(rhaD-rhaB)568 -hsdR514 -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Spiramycin
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CID:6419898) Spiramycin

  • chemical concentration= 20 ug/ml
  • normalized fitness= 4.18

OMP_AN:37044

OMP:0005098

decreased resistance to UV radiation 

Relative to: OMP_AN:27619


Genotype differences:

+ryjB(del) -DE(araD-araB)567 -DE(rhaD-rhaB)568 -hsdR514 -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:UV
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

  • chemical concentration= 6 sec
  • normalized fitness= -3.33


Additional Information

Links

References

See Help:References for how to manage references in OMPwiki.

  1. Hobbs, EC et al. (2010) Small RNAs and small proteins involved in resistance to cell envelope stress and acid shock in Escherichia coli: analysis of a bar-coded mutant collection. J. Bacteriol. 192 59-67 PubMed OMPwiki page
  2. 2.0 2.1 2.2 2.3 2.4 2.5 2.6 2.7 2.8 2.9 Nichols, RJ et al. (2011) Phenotypic landscape of a bacterial cell. Cell 144 143-56 PubMed OMPwiki page