OMP ST:71145 ! Escherichia coli K-12 GSO149

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Strain Summary

Strain Name

Escherichia coli K-12 GSO149

Synonyms
Taxon Information
  • Pangenome: Escherichia coli
  • Subspecies and/or strain: K-12
  • NCBI Taxonomy ID: 83333
Genotype

F- Lambda- rph-1 gcvB(del)::FRT-kan-FRT

Strain Reference

PMID:19734312[1]

Strain availability
Ancestry

parent:OMP ST:159 ! Escherichia coli K-12 MG1655

Annotated phenotypes


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Notes

Phenotype Annotation

Annotation ID Qualifier OMP ID OMP term name Relative phenotype information Experimental condition ECO ID ECO term name Reference Annotation Extension Notes

OMP_AN:37062

OMP:0007960

decreased population growth at low temperature

Relative to: OMP_AN:27620

Genotype differences:

+gcvB(del) -DE(araD-araB)567 -DE(rhaD-rhaB)568 -hsdR514 -lacZ4787(del)

Condition differences:
  • temperature:16C
  • medium37C
  • medium
  • temperature:16C
  • medium:LB agar
  • medium:Low temperature
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

  • chemical concentration= 16 C
  • normalized fitness= -3.93

OMP_AN:37063

OMP:0006044

decreased resistance to beta-lactam

Relative to: OMP_AN:27535

Genotype differences:

+gcvB(del) -DE(araD-araB)567 -DE(rhaD-rhaB)568 -hsdR514 -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Cefsulodin sodium salt hydrate
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:31380) Cefsulodin sodium salt hydrate

  • chemical concentration= 12 ug/ml
  • normalized fitness= -3.14

OMP_AN:37064

OMP:0007249

decreased resistance to a chemical

Relative to: OMP_AN:27514

Genotype differences:

+gcvB(del) -DE(araD-araB)567 -DE(rhaD-rhaB)568 -hsdR514 -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Chlorpromazine hydrochloride
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:3649) Chlorpromazine hydrochloride

  • chemical concentration= 6 uM
  • normalized fitness= -4.14

OMP_AN:37065

OMP:0007886

decreased resistance to a metal

Relative to: OMP_AN:27579

Genotype differences:

+gcvB(del) -DE(araD-araB)567 -DE(rhaD-rhaB)568 -hsdR514 -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Copper stress-CuCl2 dihydrate
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:86318) Copper stress-CuCl2 dihydrate

  • chemical concentration= 4 mM (LB)
  • normalized fitness= -4.95

OMP_AN:37066

OMP:0007376

increased resistance to an alkylating agent

Relative to: OMP_AN:27502

Genotype differences:

+gcvB(del) -DE(araD-araB)567 -DE(rhaD-rhaB)568 -hsdR514 -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:methyl methanesulfonate (MMS)
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:25255) methyl methanesulfonate (MMS)

  • chemical concentration= 0.05%
  • normalized fitness= 3.82

OMP_AN:37067

OMP:0007249

decreased resistance to a chemical

Relative to: OMP_AN:27510

Genotype differences:

+gcvB(del) -DE(araD-araB)567 -DE(rhaD-rhaB)568 -hsdR514 -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Mitomycin C
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:27504) Mitomycin C

  • chemical concentration= 0.1 ug/ml
  • normalized fitness= -3.24

OMP_AN:37068

OMP:0007886

decreased resistance to a metal

Relative to: OMP_AN:27580

Genotype differences:

+gcvB(del) -DE(araD-araB)567 -DE(rhaD-rhaB)568 -hsdR514 -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Nickel stress-Ni(II)Cl2 hexahydrate
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:53542) Nickel stress-Ni(II)Cl2 hexahydrate

  • chemical concentration= 1 mM (LB)
  • normalized fitness= -4.77

OMP_AN:37069

OMP:0007878

increased resistance to an antimicrobial agent

Relative to: OMP_AN:27503

Genotype differences:

+gcvB(del) -DE(araD-araB)567 -DE(rhaD-rhaB)568 -hsdR514 -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Nitrofurantoin
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:71415) Nitrofurantoin

  • chemical concentration= 1 ug/ml
  • normalized fitness= 4.22

OMP_AN:37070

OMP:0007249

decreased resistance to a chemical

Relative to: OMP_AN:27574

Genotype differences:

+gcvB(del) -DE(araD-araB)567 -DE(rhaD-rhaB)568 -hsdR514 -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Paraquat dichloride
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:28786) Paraquat dichloride

  • chemical concentration= 10 uM
  • normalized fitness= -5.22

OMP_AN:37071

OMP:0007879

decreased resistance to an antimicrobial agent

Relative to: OMP_AN:27546

Genotype differences:

+gcvB(del) -DE(araD-araB)567 -DE(rhaD-rhaB)568 -hsdR514 -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Pyocyanin
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:8653) Pyocyanin

  • chemical concentration= 10 ug/ml
  • normalized fitness= -5.59

OMP_AN:37072

OMP:0007903

decreased resistance to bile acids

Relative to: OMP_AN:27584

Genotype differences:

+gcvB(del) -DE(araD-araB)567 -DE(rhaD-rhaB)568 -hsdR514 -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Taurocholic acid sodium salt hydrate
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CID:23687511) Taurocholic acid sodium salt hydrate

  • chemical concentration= 0.5 %
  • normalized fitness= -3.68

OMP_AN:37073

OMP:0007878

increased resistance to an antimicrobial agent

Relative to: OMP_AN:27588

Genotype differences:

+gcvB(del) -DE(araD-araB)567 -DE(rhaD-rhaB)568 -hsdR514 -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Triclosan/Irgasan
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:164200) Triclosan/Irgasan

  • chemical concentration= 0.05 ug/ml
  • normalized fitness= 4.74

OMP_AN:37074

OMP:0007896

decreased resistance to a surfactant

Relative to: OMP_AN:27583

Genotype differences:

+gcvB(del) -DE(araD-araB)567 -DE(rhaD-rhaB)568 -hsdR514 -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Triton X-100
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:9750) Triton X-100

  • chemical concentration= 0.2 %
  • normalized fitness= -5

OMP_AN:37075

OMP:0007878

increased resistance to an antimicrobial agent

Relative to: OMP_AN:27558

Genotype differences:

+gcvB(del) -DE(araD-araB)567 -DE(rhaD-rhaB)568 -hsdR514 -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:D-Cycloserine
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:40009) D-Cycloserine

  • chemical concentration= 16 ug/ml
  • normalized fitness= 4.06

OMP_AN:37076

OMP:0007913

increased resistance to a chelator

Relative to: OMP_AN:27508

Genotype differences:

+gcvB(del) -DE(araD-araB)567 -DE(rhaD-rhaB)568 -hsdR514 -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:EGTA
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:30740) EGTA

  • chemical concentration= 1 mM
  • normalized fitness= 3.42

OMP_AN:37077

OMP:0007249

decreased resistance to a chemical

Relative to: OMP_AN:27569

Genotype differences:

+gcvB(del) -DE(araD-araB)567 -DE(rhaD-rhaB)568 -hsdR514 -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:EtOH
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:16236) EtOH

  • chemical concentration= 6 %
  • normalized fitness= -3.27

OMP_AN:37078

OMP:0007521

decreased population growth in high osmolarity

Relative to: OMP_AN:27595

Genotype differences:

+gcvB(del) -DE(araD-araB)567 -DE(rhaD-rhaB)568 -hsdR514 -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:NaCl
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:26710) NaCl

  • chemical concentration= 450 mM
  • normalized fitness= -3.76

OMP_AN:37079

OMP:0007878

increased resistance to an antimicrobial agent

Relative to: OMP_AN:27504

Genotype differences:

+gcvB(del) -DE(araD-araB)567 -DE(rhaD-rhaB)568 -hsdR514 -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Novobiocin sodium salt
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:31924) Novobiocin sodium salt

  • chemical concentration= 6 ug/ml
  • normalized fitness= 4.66

OMP_AN:37080

OMP:0006057

decreased resistance to aminoglycoside

Relative to: OMP_AN:27527

Genotype differences:

+gcvB(del) -DE(araD-araB)567 -DE(rhaD-rhaB)568 -hsdR514 -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Spectinomycin dihydrochloride pentahydrate
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:9217) Spectinomycin dihydrochloride pentahydrate

  • chemical concentration= 4 ug/ml
  • normalized fitness= -3.32

OMP_AN:37081

OMP:0006036

decreased resistance to macrolide

Relative to: OMP_AN:27521

Genotype differences:

+gcvB(del) -DE(araD-araB)567 -DE(rhaD-rhaB)568 -hsdR514 -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Spiramycin
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CID:6419898) Spiramycin

  • chemical concentration= 5 ug/ml
  • normalized fitness= -3.85
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Additional Information

Links

References

See Help:References for how to manage references in OMPwiki.

  1. ↑ Hobbs, EC et al. (2010) Small RNAs and small proteins involved in resistance to cell envelope stress and acid shock in Escherichia coli: analysis of a bar-coded mutant collection. J. Bacteriol. 192 59-67 PubMed OMPwiki page
  2. ↑ 2.00 2.01 2.02 2.03 2.04 2.05 2.06 2.07 2.08 2.09 2.10 2.11 2.12 2.13 2.14 2.15 2.16 2.17 2.18 2.19 Nichols, RJ et al. (2011) Phenotypic landscape of a bacterial cell. Cell 144 143-56 PubMed OMPwiki page
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