OMP ST:71143 ! Escherichia coli K-12 GSO147

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Strain Summary

Strain Name

Escherichia coli K-12 GSO147

Synonyms
Taxon Information
  • Pangenome: Escherichia coli
  • Subspecies and/or strain: K-12
  • NCBI Taxonomy ID: 83333
Genotype

F- Lambda- rph-1 dsrA(del)::FRT-kan-FRT

Strain Reference

PMID:19734312[1]

Strain availability
Ancestry

parent:OMP ST:159 ! Escherichia coli K-12 MG1655

Annotated phenotypes


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Notes

Phenotype Annotation

Annotation ID Qualifier OMP ID OMP term name Relative phenotype information Experimental condition ECO ID ECO term name Reference Annotation Extension Notes

OMP_AN:37018

OMP:0007879

decreased resistance to an antimicrobial agent

Relative to: OMP_AN:27557

Genotype differences:

+dsrA(del) -DE(araD-araB)567 -DE(rhaD-rhaB)568 -hsdR514 -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:A22
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CID:2830968) A22

  • chemical concentration= 5 ug/ml
  • normalized fitness= -3.26

OMP_AN:37019

OMP:0007879

decreased resistance to an antimicrobial agent

Relative to: OMP_AN:27552

Genotype differences:

+dsrA(del) -DE(araD-araB)567 -DE(rhaD-rhaB)568 -hsdR514 -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Azidothymidine
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:10110) Azidothymidine

  • chemical concentration= 1 ng/ml
  • normalized fitness= -4.43

OMP_AN:37020

OMP:0007376

increased resistance to an alkylating agent

Relative to: OMP_AN:27502

Genotype differences:

+dsrA(del) -DE(araD-araB)567 -DE(rhaD-rhaB)568 -hsdR514 -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:methyl methanesulfonate (MMS)
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:25255) methyl methanesulfonate (MMS)

  • chemical concentration= 0.05%
  • normalized fitness= 3.81

OMP_AN:37021

OMP:0006044

decreased resistance to beta-lactam

Relative to: OMP_AN:27537

Genotype differences:

+dsrA(del) -DE(araD-araB)567 -DE(rhaD-rhaB)568 -hsdR514 -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Mecillinam
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:51208) Mecillinam

  • chemical concentration= 0.09 ug/ml
  • normalized fitness= -3.2

OMP_AN:37022

OMP:0007886

decreased resistance to a metal

Relative to: OMP_AN:27580

Genotype differences:

+dsrA(del) -DE(araD-araB)567 -DE(rhaD-rhaB)568 -hsdR514 -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Nickel stress-Ni(II)Cl2 hexahydrate
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:53542) Nickel stress-Ni(II)Cl2 hexahydrate

  • chemical concentration= 0.1 mM (LB)
  • normalized fitness= -3.85

OMP_AN:37023

OMP:0006045

increased resistance to beta-lactam compound

Relative to: OMP_AN:27596

Genotype differences:

+dsrA(del) -DE(araD-araB)567 -DE(rhaD-rhaB)568 -hsdR514 -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Cefaclor
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:3478) Cefaclor

  • chemical concentration= 1 ug/ml
  • normalized fitness= 3.8

OMP_AN:37024

OMP:0007249

decreased resistance to a chemical

Relative to: OMP_AN:27569

Genotype differences:

+dsrA(del) -DE(araD-araB)567 -DE(rhaD-rhaB)568 -hsdR514 -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:EtOH
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:16236) EtOH

  • chemical concentration= 6 %
  • normalized fitness= -4.3

OMP_AN:37025

OMP:0006040

decreased resistance to quinolone

Relative to: OMP_AN:27542

Genotype differences:

+dsrA(del) -DE(araD-araB)567 -DE(rhaD-rhaB)568 -hsdR514 -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Norfloxacin
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:100246) Norfloxacin

  • chemical concentration= 0.02 ug/ml
  • normalized fitness= -3.72
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Additional Information

Links

References

See Help:References for how to manage references in OMPwiki.

  1. ↑ Hobbs, EC et al. (2010) Small RNAs and small proteins involved in resistance to cell envelope stress and acid shock in Escherichia coli: analysis of a bar-coded mutant collection. J. Bacteriol. 192 59-67 PubMed OMPwiki page
  2. ↑ 2.0 2.1 2.2 2.3 2.4 2.5 2.6 2.7 Nichols, RJ et al. (2011) Phenotypic landscape of a bacterial cell. Cell 144 143-56 PubMed OMPwiki page
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