OMP ST:71122 ! Escherichia coli K-12 BW25113 dnaA::SPA-kan

Strain Summary

Strain Name	Escherichia coli K-12 BW25113 dnaA::SPA-kan
Synonyms
Taxon Information	Pangenome: Escherichia coli Subspecies and/or strain: K-12 NCBI Taxonomy ID: 83333
Genotype	DE(araD-araB)567 DE(rhaD-rhaB)568 F- Lambda- hsdR514 lacZ4787(del)(::rrnB-3) rph-1 dnaA::SPA-kan
Strain Reference	PMID:21185072
Strain availability
Ancestry	parent:OMP ST:800 ! Escherichia coli K-12 BW25113
Annotated phenotypes
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Phenotype Annotation

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Annotation ID	Qualifier	OMP ID	OMP term name	Relative phenotype information	Experimental condition	ECO ID	ECO term name	Reference	Annotation Extension	Notes
OMP_AN:37223		OMP:0007960	decreased population growth at low temperature	Relative to: OMP_AN:27620 Genotype differences: +lacZ4787(del)( +dnaA -lacZ4787(del) Condition differences: temperature:16C medium37C medium	temperature:16C medium:LB agar medium:Low temperature	ECO:0007005	high throughput direct assay evidence used in manual assertion	PMID:21185072		chemical concentration= 16 C normalized fitness= -6.41
OMP_AN:37224		OMP:0006040	decreased resistance to quinolone	Relative to: OMP_AN:27539 Genotype differences: +lacZ4787(del)( +dnaA -lacZ4787(del) Condition differences: no differences	temperature:37C medium:LB agar medium:Ciprofloxacin hydrochloride hydrate	ECO:0007005	high throughput direct assay evidence used in manual assertion	PMID:21185072	towards some (CHEBI:59936) Ciprofloxacin hydrochloride hydrate	chemical concentration= 0.008 ug/ml normalized fitness= -4.31
OMP_AN:37225		OMP:0007075	decreased growth factor requirement	Relative to: OMP_AN:27599 Genotype differences: +lacZ4787(del)( +dnaA -lacZ4787(del) Condition differences: no differences	temperature:37C medium:MOPS medium:Iron starvation-FeSO4·7H2O	ECO:0007005	high throughput direct assay evidence used in manual assertion	PMID:21185072	towards some (CHEBI:75836) Iron starvation-FeSO4·7H2O	chemical concentration= 2uM (MOPS; normal 100uM) normalized fitness= -3.3
OMP_AN:37226		OMP:0007937	decreased resistance to nutrient limitation	Relative to: OMP_AN:27609 Genotype differences: +lacZ4787(del)( +dnaA -lacZ4787(del) Condition differences: no differences	temperature:37C medium:MOPS medium:Iron starvation-FeSO4·7H2O	ECO:0007005	high throughput direct assay evidence used in manual assertion	PMID:21185072	towards some (CHEBI:75836) Iron starvation-FeSO4·7H2O	chemical concentration= 2uM (MOPS; normal 100uM) normalized fitness= -3.3
OMP_AN:37227		OMP:0007879	decreased resistance to an antimicrobial agent	Relative to: OMP_AN:27500 Genotype differences: +lacZ4787(del)( +dnaA -lacZ4787(del) Condition differences: no differences	temperature:37C medium:LB agar medium:Trimethoprim	ECO:0007005	high throughput direct assay evidence used in manual assertion	PMID:21185072	towards some (CHEBI:45924) Trimethoprim	chemical concentration= 0.4 ug/ml normalized fitness= -4.42

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References

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