OMP ST:71086 ! Escherichia coli K-12 BW25113 bamD::DAS-kan

Strain Summary

Strain Name	Escherichia coli K-12 BW25113 bamD::DAS-kan
Synonyms
Taxon Information	Pangenome: Escherichia coli Subspecies and/or strain: K-12 NCBI Taxonomy ID: 83333
Genotype	DE(araD-araB)567 DE(rhaD-rhaB)568 F- Lambda- hsdR514 lacZ4787(del)(::rrnB-3) rph-1 bamD::DAS-kan
Strain Reference	PMID:21185072
Strain availability
Ancestry	parent:OMP ST:800 ! Escherichia coli K-12 BW25113
Annotated phenotypes
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Phenotype Annotation

Annotation ID	Qualifier	OMP ID	OMP term name	Relative phenotype information	Experimental condition	ECO ID	ECO term name	Reference	Annotation Extension	Notes
OMP_AN:37168		OMP:0007896	decreased resistance to a surfactant	Relative to: OMP_AN:27582 Genotype differences: +lacZ4787(del)( +bamD -lacZ4787(del) Condition differences: no differences	temperature:37C medium:LB agar medium:SDS	ECO:0007005	high throughput direct assay evidence used in manual assertion	PMID:21185072	towards some (CHEBI:8984) SDS	chemical concentration= 1 % normalized fitness= -3.41
OMP_AN:37169		OMP:0007249	decreased resistance to a chemical	Relative to: OMP_AN:27569 Genotype differences: +lacZ4787(del)( +bamD -lacZ4787(del) Condition differences: no differences	temperature:37C medium:LB agar medium:EtOH	ECO:0007005	high throughput direct assay evidence used in manual assertion	PMID:21185072	towards some (CHEBI:16236) EtOH	chemical concentration= 4 % normalized fitness= -3.42
OMP_AN:37170		OMP:0007903	decreased resistance to bile acids	Relative to: OMP_AN:27585 Genotype differences: +lacZ4787(del)( +bamD -lacZ4787(del) Condition differences: no differences	temperature:37C medium:LB agar medium:Cholate sodium hydrate	ECO:0007005	high throughput direct assay evidence used in manual assertion	PMID:21185072	towards some (CID:23679061) Cholate sodium hydrate	chemical concentration= 0.5 % normalized fitness= -3.72
OMP_AN:37171		OMP:0006037	increased resistance to macrolide	Relative to: OMP_AN:27519 Genotype differences: +lacZ4787(del)( +bamD -lacZ4787(del) Condition differences: no differences	temperature:37C medium:LB agar medium:Clarithromycin	ECO:0007005	high throughput direct assay evidence used in manual assertion	PMID:21185072	towards some (CHEBI:3732) Clarithromycin	chemical concentration= 5 ug/ml normalized fitness= 3.37
OMP_AN:37172		OMP:0007912	decreased resistance to a chelator	Relative to: OMP_AN:27507 Genotype differences: +lacZ4787(del)( +bamD -lacZ4787(del) Condition differences: no differences	temperature:37C medium:LB agar medium:EDTA disodium salt dihydrate	ECO:0007005	high throughput direct assay evidence used in manual assertion	PMID:21185072	towards some (CHEBI:64758) EDTA disodium salt dihydrate	chemical concentration= 0.1 mM normalized fitness= -3.76
OMP_AN:37173		OMP:0007886	decreased resistance to a metal	Relative to: OMP_AN:27580 Genotype differences: +lacZ4787(del)( +bamD -lacZ4787(del) Condition differences: no differences	temperature:37C medium:LB agar medium:Nickel stress-Ni(II)Cl2 hexahydrate	ECO:0007005	high throughput direct assay evidence used in manual assertion	PMID:21185072	towards some (CHEBI:53542) Nickel stress-Ni(II)Cl2 hexahydrate	chemical concentration= 0.1 mM (LB) normalized fitness= -4.27
OMP_AN:37174		OMP:0007250	increased resistance to a chemical	Relative to: OMP_AN:27573 Genotype differences: +lacZ4787(del)( +bamD -lacZ4787(del) Condition differences: no differences	temperature:37C medium:LB agar medium:Procaine hydrochloride	ECO:0007005	high throughput direct assay evidence used in manual assertion	PMID:21185072	towards some (CHEBI:8431) Procaine hydrochloride	chemical concentration= 5 mM normalized fitness= 3.4
OMP_AN:37175		OMP:0007879	decreased resistance to an antimicrobial agent	Relative to: OMP_AN:27566 Genotype differences: +lacZ4787(del)( +bamD -lacZ4787(del) Condition differences: no differences	temperature:37C medium:LB agar medium:Vancomycin HCl hydrate	ECO:0007005	high throughput direct assay evidence used in manual assertion	PMID:21185072	towards some (CHEBI:9932) Vancomycin HCl hydrate	chemical concentration= 10 ug/ml normalized fitness= -4.06
OMP_AN:37176		OMP:0007249	decreased resistance to a chemical	Relative to: OMP_AN:27568 Genotype differences: +lacZ4787(del)( +bamD -lacZ4787(del) Condition differences: no differences	temperature:37C medium:LB agar medium:(±)-Verapamil hydrochloride	ECO:0007005	high throughput direct assay evidence used in manual assertion	PMID:21185072	towards some (CHEBI:53188) (±)-Verapamil hydrochloride	chemical concentration= 0.5 mM normalized fitness= -3.57
OMP_AN:37177		OMP:0007879	decreased resistance to an antimicrobial agent	Relative to: OMP_AN:27557 Genotype differences: +lacZ4787(del)( +bamD -lacZ4787(del) Condition differences: no differences	temperature:37C medium:LB agar medium:A22	ECO:0007005	high throughput direct assay evidence used in manual assertion	PMID:21185072	towards some (CID:2830968) A22	chemical concentration= 15 ug/ml normalized fitness= -4.49
OMP_AN:37178		OMP:0007249	decreased resistance to a chemical	Relative to: OMP_AN:27501 Genotype differences: +lacZ4787(del)( +bamD -lacZ4787(del) Condition differences: no differences	temperature:37C medium:LB agar medium:Dibucaine	ECO:0007005	high throughput direct assay evidence used in manual assertion	PMID:21185072	towards some (CHEBI:247956) Dibucaine	chemical concentration= 0.4 mM normalized fitness= -3.31
OMP_AN:37179		OMP:0007879	decreased resistance to an antimicrobial agent	Relative to: OMP_AN:27555 Genotype differences: +lacZ4787(del)( +bamD -lacZ4787(del) Condition differences: no differences	temperature:37C medium:LB agar medium:Fosfomycin disodium salt	ECO:0007005	high throughput direct assay evidence used in manual assertion	PMID:21185072	towards some (CID:6093162) Fosfomycin disodium salt	chemical concentration= 1 ug/ml normalized fitness= -3.28
OMP_AN:37180		OMP:0007879	decreased resistance to an antimicrobial agent	Relative to: OMP_AN:27590 Genotype differences: +lacZ4787(del)( +bamD -lacZ4787(del) Condition differences: no differences	temperature:37C medium:LB agar medium:Fosfomycin (CID:6093162) +Glucose-6-phosphate (CHEBI:4170)	ECO:0007005	high throughput direct assay evidence used in manual assertion	PMID:21185072	towards some (CID:6093162 + CHEBI:4170) Fosfomycin (CID:6093162) +Glucose-6-phosphate (CHEBI:4170)	chemical concentration= 0.2 ug/ml (in 50 mg/ml G6P) normalized fitness= -9.7
OMP_AN:37181		OMP:0006045	increased resistance to beta-lactam compound	Relative to: OMP_AN:27537 Genotype differences: +lacZ4787(del)( +bamD -lacZ4787(del) Condition differences: no differences	temperature:37C medium:LB agar medium:Mecillinam	ECO:0007005	high throughput direct assay evidence used in manual assertion	PMID:21185072	towards some (CHEBI:51208) Mecillinam	chemical concentration= 0.12 ug/ml normalized fitness= 3.49
OMP_AN:37182		OMP:0006061	decreased resistance to tetracycline	Relative to: OMP_AN:27522 Genotype differences: +lacZ4787(del)( +bamD -lacZ4787(del) Condition differences: no differences	temperature:37C medium:LB agar medium:Doxycycline hyclate	ECO:0007005	high throughput direct assay evidence used in manual assertion	PMID:21185072	towards some (CHEBI:34730) Doxycycline hyclate	chemical concentration= 0.75 ug/ml normalized fitness= -3.88
OMP_AN:37183		OMP:0007074	increased growth factor requirement	Relative to: OMP_AN:27599 Genotype differences: +lacZ4787(del)( +bamD -lacZ4787(del) Condition differences: no differences	temperature:37C medium:MOPS medium:Iron starvation-FeSO4·7H2O	ECO:0007005	high throughput direct assay evidence used in manual assertion	PMID:21185072	towards some (CHEBI:75836) Iron starvation-FeSO4·7H2O	chemical concentration= 2uM (MOPS; normal 100uM) normalized fitness= 3.41
OMP_AN:37184		OMP:0007936	increased resistance to nutrient limitation	Relative to: OMP_AN:27609 Genotype differences: +lacZ4787(del)( +bamD -lacZ4787(del) Condition differences: no differences	temperature:37C medium:MOPS medium:Iron starvation-FeSO4·7H2O	ECO:0007005	high throughput direct assay evidence used in manual assertion	PMID:21185072	towards some (CHEBI:75836) Iron starvation-FeSO4·7H2O	chemical concentration= 2uM (MOPS; normal 100uM) normalized fitness= 3.41
OMP_AN:37185		OMP:0006041	increased resistance to quinolone	Relative to: OMP_AN:27541 Genotype differences: +lacZ4787(del)( +bamD -lacZ4787(del) Condition differences: no differences	temperature:37C medium:LB agar medium:Nalidixic acid sodium salt	ECO:0007005	high throughput direct assay evidence used in manual assertion	PMID:21185072	towards some (CID:3864541) Nalidixic acid sodium salt	chemical concentration= 2 ug/ml normalized fitness= 3.41
OMP_AN:37186		OMP:0007879	decreased resistance to an antimicrobial agent	Relative to: OMP_AN:27504 Genotype differences: +lacZ4787(del)( +bamD -lacZ4787(del) Condition differences: no differences	temperature:37C medium:LB agar medium:Novobiocin sodium salt	ECO:0007005	high throughput direct assay evidence used in manual assertion	PMID:21185072	towards some (CHEBI:31924) Novobiocin sodium salt	chemical concentration= 12 ug/ml normalized fitness= -3.6
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References

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