OMP ST:71078 ! Escherichia coli K-12 BW25113 accD::SPA-kan

Strain Summary

Strain Name	Escherichia coli K-12 BW25113 accD::SPA-kan
Synonyms
Taxon Information	Pangenome: Escherichia coli Subspecies and/or strain: K-12 NCBI Taxonomy ID: 83333
Genotype	DE(araD-araB)567 DE(rhaD-rhaB)568 F- Lambda- hsdR514 lacZ4787(del)(::rrnB-3) rph-1 accD::SPA-kan
Strain Reference	PMID:21185072
Strain availability
Ancestry	parent:OMP ST:800 ! Escherichia coli K-12 BW25113
Annotated phenotypes
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Phenotype Annotation

Annotation ID	Qualifier	OMP ID	OMP term name	Relative phenotype information	Experimental condition	ECO ID	ECO term name	Reference	Annotation Extension	Notes
OMP_AN:37118		OMP:0007075	decreased growth factor requirement	Relative to: OMP_AN:27601 Genotype differences: +lacZ4787(del)( +accD -lacZ4787(del) Condition differences: no differences	temperature:37C medium:M9 medium:Sodium Acetate anhydrous	ECO:0007005	high throughput direct assay evidence used in manual assertion	PMID:21185072	towards some (CHEBI:32954) Sodium Acetate anhydrous	chemical concentration= 0.6% normalized fitness= -4.95
OMP_AN:37119		OMP:0007070	decreased organic carbon source utilization	Relative to: OMP_AN:27611 Genotype differences: +lacZ4787(del)( +accD -lacZ4787(del) Condition differences: no differences	temperature:37C medium:M9 medium:Sodium Acetate anhydrous	ECO:0007005	high throughput direct assay evidence used in manual assertion	PMID:21185072	towards some (CHEBI:32954) Sodium Acetate anhydrous	chemical concentration= 0.6% normalized fitness= -4.95
OMP_AN:37120		OMP:0006036	decreased resistance to macrolide	Relative to: OMP_AN:27518 Genotype differences: +lacZ4787(del)( +accD -lacZ4787(del) Condition differences: no differences	temperature:37C medium:LB agar medium:Azithromycin	ECO:0007005	high throughput direct assay evidence used in manual assertion	PMID:21185072	towards some (CHEBI:2955) Azithromycin	chemical concentration= 0.1 ug/ml normalized fitness= -4.37
OMP_AN:37121		OMP:0007903	decreased resistance to bile acids	Relative to: OMP_AN:27585 Genotype differences: +lacZ4787(del)( +accD -lacZ4787(del) Condition differences: no differences	temperature:37C medium:LB agar medium:Cholate sodium hydrate	ECO:0007005	high throughput direct assay evidence used in manual assertion	PMID:21185072	towards some (CID:23679061) Cholate sodium hydrate	chemical concentration= 0.1 % normalized fitness= -5.05
OMP_AN:37122		OMP:0007886	decreased resistance to a metal	Relative to: OMP_AN:27579 Genotype differences: +lacZ4787(del)( +accD -lacZ4787(del) Condition differences: no differences	temperature:37C medium:LB agar medium:Copper stress-CuCl2 dihydrate	ECO:0007005	high throughput direct assay evidence used in manual assertion	PMID:21185072	towards some (CHEBI:86318) Copper stress-CuCl2 dihydrate	chemical concentration= 1 mM (LB) normalized fitness= -4.95
OMP_AN:37123		OMP:0007879	decreased resistance to an antimicrobial agent	Relative to: OMP_AN:27506 Genotype differences: +lacZ4787(del)( +accD -lacZ4787(del) Condition differences: no differences	temperature:37C medium:LB agar medium:Doxorubicin hydrochloride	ECO:0007005	high throughput direct assay evidence used in manual assertion	PMID:21185072	towards some (CHEBI:31522) Doxorubicin hydrochloride	chemical concentration= 10 ug/ml normalized fitness= -3.68
OMP_AN:37124		OMP:0007075	decreased growth factor requirement	Relative to: OMP_AN:27606 Genotype differences: +lacZ4787(del)( +accD -lacZ4787(del) Condition differences: no differences	temperature:37C medium:M9 medium:N-Acetyl-D-glucosamine	ECO:0007005	high throughput direct assay evidence used in manual assertion	PMID:21185072	towards some (CHEBI:506227) N-Acetyl-D-glucosamine	chemical concentration= 0.15% normalized fitness= -4.01
OMP_AN:37125		OMP:0007070	decreased organic carbon source utilization	Relative to: OMP_AN:27616 Genotype differences: +lacZ4787(del)( +accD -lacZ4787(del) Condition differences: no differences	temperature:37C medium:M9 medium:N-Acetyl-D-glucosamine	ECO:0007005	high throughput direct assay evidence used in manual assertion	PMID:21185072	towards some (CHEBI:506227) N-Acetyl-D-glucosamine	chemical concentration= 0.15% normalized fitness= -4.01
OMP_AN:37126		OMP:0007075	decreased growth factor requirement	Relative to: OMP_AN:27603 Genotype differences: +lacZ4787(del)( +accD -lacZ4787(del) Condition differences: no differences	temperature:37C medium:M9 medium:D-Glucose anhydrous	ECO:0007005	high throughput direct assay evidence used in manual assertion	PMID:21185072	towards some (CHEBI:42758) D-Glucose anhydrous	chemical concentration= 0.2% normalized fitness= -5.19
OMP_AN:37127		OMP:0007070	decreased organic carbon source utilization	Relative to: OMP_AN:27613 Genotype differences: +lacZ4787(del)( +accD -lacZ4787(del) Condition differences: no differences	temperature:37C medium:M9 medium:D-Glucose anhydrous	ECO:0007005	high throughput direct assay evidence used in manual assertion	PMID:21185072	towards some (CHEBI:42758) D-Glucose anhydrous	chemical concentration= 0.2% normalized fitness= -5.19
OMP_AN:37128		OMP:0007075	decreased growth factor requirement	Relative to: OMP_AN:27605 Genotype differences: +lacZ4787(del)( +accD -lacZ4787(del) Condition differences: no differences	temperature:37C medium:M9 medium:D-(+) Maltose monohydrate	ECO:0007005	high throughput direct assay evidence used in manual assertion	PMID:21185072	towards some (CID:23615261) D-(+) Maltose monohydrate	chemical concentration= 0.1% normalized fitness= -2.94
OMP_AN:37129		OMP:0007070	decreased organic carbon source utilization	Relative to: OMP_AN:27615 Genotype differences: +lacZ4787(del)( +accD -lacZ4787(del) Condition differences: no differences	temperature:37C medium:M9 medium:D-(+) Maltose monohydrate	ECO:0007005	high throughput direct assay evidence used in manual assertion	PMID:21185072	towards some (CID:23615261) D-(+) Maltose monohydrate	chemical concentration= 0.1% normalized fitness= -2.94
OMP_AN:37130		OMP:0007886	decreased resistance to a metal	Relative to: OMP_AN:27580 Genotype differences: +lacZ4787(del)( +accD -lacZ4787(del) Condition differences: no differences	temperature:37C medium:LB agar medium:Nickel stress-Ni(II)Cl2 hexahydrate	ECO:0007005	high throughput direct assay evidence used in manual assertion	PMID:21185072	towards some (CHEBI:53542) Nickel stress-Ni(II)Cl2 hexahydrate	chemical concentration= 0.1 mM (LB) normalized fitness= -4.47
OMP_AN:37131		OMP:0007249	decreased resistance to a chemical	Relative to: OMP_AN:27593 Genotype differences: +lacZ4787(del)( +accD -lacZ4787(del) Condition differences: no differences	temperature:37C medium:LB agar medium:(-)-Norepinephrine	ECO:0007005	high throughput direct assay evidence used in manual assertion	PMID:21185072	towards some (CHEBI:18357) (-)-Norepinephrine	chemical concentration= 1000 uM normalized fitness= -4.49
OMP_AN:37132		OMP:0007249	decreased resistance to a chemical	Relative to: OMP_AN:27574 Genotype differences: +lacZ4787(del)( +accD -lacZ4787(del) Condition differences: no differences	temperature:37C medium:LB agar medium:Paraquat dichloride	ECO:0007005	high throughput direct assay evidence used in manual assertion	PMID:21185072	towards some (CHEBI:28786) Paraquat dichloride	chemical concentration= 0.2 uM normalized fitness= -4.91
OMP_AN:37133		OMP:0006041	increased resistance to quinolone	Relative to: OMP_AN:27543 Genotype differences: +lacZ4787(del)( +accD -lacZ4787(del) Condition differences: no differences	temperature:37C medium:LB agar medium:Streptonigrin	ECO:0007005	high throughput direct assay evidence used in manual assertion	PMID:21185072	towards some (CHEBI:9287) Streptonigrin	chemical concentration= 0.1 ug/ml normalized fitness= 3.79
OMP_AN:37134		OMP:0007075	decreased growth factor requirement	Relative to: OMP_AN:27607 Genotype differences: +lacZ4787(del)( +accD -lacZ4787(del) Condition differences: no differences	temperature:37C medium:M9 medium:Succinic acid	ECO:0007005	high throughput direct assay evidence used in manual assertion	PMID:21185072	towards some (CHEBI:15741) Succinic acid	chemical concentration= 0.3% normalized fitness= -3.57
OMP_AN:37135		OMP:0007070	decreased organic carbon source utilization	Relative to: OMP_AN:27617 Genotype differences: +lacZ4787(del)( +accD -lacZ4787(del) Condition differences: no differences	temperature:37C medium:M9 medium:Succinic acid	ECO:0007005	high throughput direct assay evidence used in manual assertion	PMID:21185072	towards some (CHEBI:15741) Succinic acid	chemical concentration= 0.3% normalized fitness= -3.57
OMP_AN:37136		OMP:0007075	decreased growth factor requirement	Relative to: OMP_AN:27598 Genotype differences: +lacZ4787(del)( +accD -lacZ4787(del) Condition differences: no differences	temperature:37C medium:MOPS medium:Iron excess-FeSO4 heptahydride	ECO:0007005	high throughput direct assay evidence used in manual assertion	PMID:21185072	towards some (CHEBI:75836) Iron excess-FeSO4 heptahydride	chemical concentration= 1mM (MOPS; normal 100uM) normalized fitness= -3.8
OMP_AN:37137		OMP:0007886	decreased resistance to a metal	Relative to: OMP_AN:27608 Genotype differences: +lacZ4787(del)( +accD -lacZ4787(del) Condition differences: no differences	temperature:37C medium:MOPS medium:Iron excess-FeSO4 heptahydride	ECO:0007005	high throughput direct assay evidence used in manual assertion	PMID:21185072	towards some (CHEBI:75836) Iron excess-FeSO4 heptahydride	chemical concentration= 1mM (MOPS; normal 100uM) normalized fitness= -3.8
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References

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