OMP ST:71063 ! Escherichia coli K-12 BW25113 prfA::SPA-kan

Strain Summary

Strain Name	Escherichia coli K-12 BW25113 prfA::SPA-kan
Synonyms
Taxon Information	Pangenome: Escherichia coli Subspecies and/or strain: K-12 NCBI Taxonomy ID: 83333
Genotype	DE(araD-araB)567 DE(rhaD-rhaB)568 F- Lambda- hsdR514 lacZ4787(del)(::rrnB-3) rph-1 prfA::SPA-kan
Strain Reference	PMID:21185072
Strain availability
Ancestry	parent:OMP ST:800 ! Escherichia coli K-12 BW25113
Annotated phenotypes
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Phenotype Annotation

Annotation ID	Qualifier	OMP ID	OMP term name	Relative phenotype information	Experimental condition	ECO ID	ECO term name	Reference	Annotation Extension	Notes
OMP_AN:37518		OMP:0006044	decreased resistance to beta-lactam	Relative to: OMP_AN:27530 Genotype differences: +lacZ4787(del)( +prfA -lacZ4787(del) Condition differences: no differences	temperature:37C medium:LB agar medium:Amoxicillin	ECO:0007005	high throughput direct assay evidence used in manual assertion	PMID:21185072	towards some (CHEBI:2676) Amoxicillin	chemical concentration= 1 ug/ml normalized fitness= -4.25
OMP_AN:37519		OMP:0006044	decreased resistance to beta-lactam	Relative to: OMP_AN:27535 Genotype differences: +lacZ4787(del)( +prfA -lacZ4787(del) Condition differences: no differences	temperature:37C medium:LB agar medium:Cefsulodin sodium salt hydrate	ECO:0007005	high throughput direct assay evidence used in manual assertion	PMID:21185072	towards some (CHEBI:31380) Cefsulodin sodium salt hydrate	chemical concentration= 6 ug/ml normalized fitness= -3.87
OMP_AN:37520		OMP:0007249	decreased resistance to a chemical	Relative to: OMP_AN:27514 Genotype differences: +lacZ4787(del)( +prfA -lacZ4787(del) Condition differences: no differences	temperature:37C medium:LB agar medium:Chlorpromazine hydrochloride	ECO:0007005	high throughput direct assay evidence used in manual assertion	PMID:21185072	towards some (CHEBI:3649) Chlorpromazine hydrochloride	chemical concentration= 3 uM normalized fitness= -4.61
OMP_AN:37521		OMP:0006036	decreased resistance to macrolide	Relative to: OMP_AN:27520 Genotype differences: +lacZ4787(del)( +prfA -lacZ4787(del) Condition differences: no differences	temperature:37C medium:LB agar medium:Erythromycin	ECO:0007005	high throughput direct assay evidence used in manual assertion	PMID:21185072	towards some (CHEBI:42355) Erythromycin	chemical concentration= 10 ug/ml normalized fitness= -3.38
OMP_AN:37522		OMP:0007075	decreased growth factor requirement	Relative to: OMP_AN:27603 Genotype differences: +lacZ4787(del)( +prfA -lacZ4787(del) Condition differences: no differences	temperature:37C medium:M9 medium:D-Glucose anhydrous	ECO:0007005	high throughput direct assay evidence used in manual assertion	PMID:21185072	towards some (CHEBI:42758) D-Glucose anhydrous	chemical concentration= 0.2% normalized fitness= -3.62
OMP_AN:37523		OMP:0007070	decreased organic carbon source utilization	Relative to: OMP_AN:27613 Genotype differences: +lacZ4787(del)( +prfA -lacZ4787(del) Condition differences: no differences	temperature:37C medium:M9 medium:D-Glucose anhydrous	ECO:0007005	high throughput direct assay evidence used in manual assertion	PMID:21185072	towards some (CHEBI:42758) D-Glucose anhydrous	chemical concentration= 0.2% normalized fitness= -3.62
OMP_AN:37524		OMP:0006045	increased resistance to beta-lactam compound	Relative to: OMP_AN:27537 Genotype differences: +lacZ4787(del)( +prfA -lacZ4787(del) Condition differences: no differences	temperature:37C medium:LB agar medium:Mecillinam	ECO:0007005	high throughput direct assay evidence used in manual assertion	PMID:21185072	towards some (CHEBI:51208) Mecillinam	chemical concentration= 0.03 ug/ml normalized fitness= 4.9
OMP_AN:37525		OMP:0007075	decreased growth factor requirement	Relative to: OMP_AN:27600 Genotype differences: +lacZ4787(del)( +prfA -lacZ4787(del) Condition differences: no differences	temperature:37C medium:MOPS medium:NH4Cl	ECO:0007005	high throughput direct assay evidence used in manual assertion	PMID:21185072	towards some (CHEBI:31206) NH4Cl	chemical concentration= 9.5mm normalized fitness= -3.55
OMP_AN:37526		OMP:0007032	decreased utilization of inorganic nitrogen source	Relative to: OMP_AN:27610 Genotype differences: +lacZ4787(del)( +prfA -lacZ4787(del) Condition differences: no differences	temperature:37C medium:MOPS medium:NH4Cl	ECO:0007005	high throughput direct assay evidence used in manual assertion	PMID:21185072	towards some (CHEBI:31206) NH4Cl	chemical concentration= 9.5mm normalized fitness= -3.55
OMP_AN:37527		OMP:0007249	decreased resistance to a chemical	Relative to: OMP_AN:27573 Genotype differences: +lacZ4787(del)( +prfA -lacZ4787(del) Condition differences: no differences	temperature:37C medium:LB agar medium:Procaine hydrochloride	ECO:0007005	high throughput direct assay evidence used in manual assertion	PMID:21185072	towards some (CHEBI:8431) Procaine hydrochloride	chemical concentration= 10 mM normalized fitness= -4.55
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References

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