OMP ST:71058 ! Escherichia coli K-12 BW25113 acpP::SPA-kan

Strain Summary

Strain Name	Escherichia coli K-12 BW25113 acpP::SPA-kan
Synonyms
Taxon Information	Pangenome: Escherichia coli Subspecies and/or strain: K-12 NCBI Taxonomy ID: 83333
Genotype	DE(araD-araB)567 DE(rhaD-rhaB)568 F- Lambda- hsdR514 lacZ4787(del)(::rrnB-3) rph-1 acpP::SPA-kan
Strain Reference	PMID:21185072
Strain availability
Ancestry	parent:OMP ST:800 ! Escherichia coli K-12 BW25113
Annotated phenotypes
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Phenotype Annotation

Annotation ID	Qualifier	OMP ID	OMP term name	Relative phenotype information	Experimental condition	ECO ID	ECO term name	Reference	Annotation Extension	Notes
OMP_AN:37138		OMP:0007249	decreased resistance to a chemical	Relative to: OMP_AN:27594 Genotype differences: +lacZ4787(del)( +acpP -lacZ4787(del) Condition differences: no differences	temperature:37C medium:LB agar medium:SDS (CHEBI:8984) +EDTA (CHEBI:64758)	ECO:0007005	high throughput direct assay evidence used in manual assertion	PMID:21185072	towards some (CHEBI:64758 + CHEBI:8984) SDS (CHEBI:8984) +EDTA (CHEBI:64758)	chemical concentration= 0.5%/0.1mM %/0.5mM normalized fitness= -3.31
OMP_AN:37139		OMP:0007879	decreased resistance to an antimicrobial agent	Relative to: OMP_AN:27552 Genotype differences: +lacZ4787(del)( +acpP -lacZ4787(del) Condition differences: no differences	temperature:37C medium:LB agar medium:Azidothymidine	ECO:0007005	high throughput direct assay evidence used in manual assertion	PMID:21185072	towards some (CHEBI:10110) Azidothymidine	chemical concentration= 1 ng/ml normalized fitness= -3.11
OMP_AN:37140		OMP:0006036	decreased resistance to macrolide	Relative to: OMP_AN:27518 Genotype differences: +lacZ4787(del)( +acpP -lacZ4787(del) Condition differences: no differences	temperature:37C medium:LB agar medium:Azithromycin	ECO:0007005	high throughput direct assay evidence used in manual assertion	PMID:21185072	towards some (CHEBI:2955) Azithromycin	chemical concentration= 0.1 ug/ml normalized fitness= -3.87
OMP_AN:37141		OMP:0007878	increased resistance to an antimicrobial agent	Relative to: OMP_AN:27545 Genotype differences: +lacZ4787(del)( +acpP -lacZ4787(del) Condition differences: no differences	temperature:37C medium:LB agar medium:Isoniazid	ECO:0007005	high throughput direct assay evidence used in manual assertion	PMID:21185072	towards some (CHEBI:6030) Isoniazid	chemical concentration= 0.2 mM normalized fitness= 3.78
OMP_AN:37142		OMP:0007376	increased resistance to an alkylating agent	Relative to: OMP_AN:27502 Genotype differences: +lacZ4787(del)( +acpP -lacZ4787(del) Condition differences: no differences	temperature:37C medium:LB agar medium:methyl methanesulfonate (MMS)	ECO:0007005	high throughput direct assay evidence used in manual assertion	PMID:21185072	towards some (CHEBI:25255) methyl methanesulfonate (MMS)	chemical concentration= 0.05% normalized fitness= 6.16
OMP_AN:37143		OMP:0007886	decreased resistance to a metal	Relative to: OMP_AN:27580 Genotype differences: +lacZ4787(del)( +acpP -lacZ4787(del) Condition differences: no differences	temperature:37C medium:LB agar medium:Nickel stress-Ni(II)Cl2 hexahydrate	ECO:0007005	high throughput direct assay evidence used in manual assertion	PMID:21185072	towards some (CHEBI:53542) Nickel stress-Ni(II)Cl2 hexahydrate	chemical concentration= 1 mM (LB) normalized fitness= -15.24
OMP_AN:37144		OMP:0007879	decreased resistance to an antimicrobial agent	Relative to: OMP_AN:27516 Genotype differences: +lacZ4787(del)( +acpP -lacZ4787(del) Condition differences: no differences	temperature:37C medium:LB agar medium:Thiolactomycin	ECO:0007005	high throughput direct assay evidence used in manual assertion	PMID:21185072	towards some (CID:445629) Thiolactomycin	chemical concentration= 50 uM normalized fitness= -10.38
OMP_AN:37145		OMP:0007879	decreased resistance to an antimicrobial agent	Relative to: OMP_AN:27588 Genotype differences: +lacZ4787(del)( +acpP -lacZ4787(del) Condition differences: no differences	temperature:37C medium:LB agar medium:Triclosan/Irgasan	ECO:0007005	high throughput direct assay evidence used in manual assertion	PMID:21185072	towards some (CHEBI:164200) Triclosan/Irgasan	chemical concentration= 0.05 ug/ml normalized fitness= -3.14
OMP_AN:37146		OMP:0007249	decreased resistance to a chemical	Relative to: OMP_AN:27568 Genotype differences: +lacZ4787(del)( +acpP -lacZ4787(del) Condition differences: no differences	temperature:37C medium:LB agar medium:(±)-Verapamil hydrochloride	ECO:0007005	high throughput direct assay evidence used in manual assertion	PMID:21185072	towards some (CHEBI:53188) (±)-Verapamil hydrochloride	chemical concentration= 1 mM normalized fitness= -3.18
OMP_AN:37147		OMP:0007249	decreased resistance to a chemical	Relative to: OMP_AN:27569 Genotype differences: +lacZ4787(del)( +acpP -lacZ4787(del) Condition differences: no differences	temperature:37C medium:LB agar medium:EtOH	ECO:0007005	high throughput direct assay evidence used in manual assertion	PMID:21185072	towards some (CHEBI:16236) EtOH	chemical concentration= 6 % normalized fitness= -4.95
OMP_AN:37148		OMP:0007521	decreased population growth in high osmolarity	Relative to: OMP_AN:27595 Genotype differences: +lacZ4787(del)( +acpP -lacZ4787(del) Condition differences: no differences	temperature:37C medium:LB agar medium:NaCl	ECO:0007005	high throughput direct assay evidence used in manual assertion	PMID:21185072	towards some (CHEBI:26710) NaCl	chemical concentration= 600 mM normalized fitness= -5.37
OMP_AN:37149		OMP:0006040	decreased resistance to quinolone	Relative to: OMP_AN:27542 Genotype differences: +lacZ4787(del)( +acpP -lacZ4787(del) Condition differences: no differences	temperature:37C medium:LB agar medium:Norfloxacin	ECO:0007005	high throughput direct assay evidence used in manual assertion	PMID:21185072	towards some (CHEBI:100246) Norfloxacin	chemical concentration= 0.04 ug/ml normalized fitness= -3.21
OMP_AN:37150		OMP:0007879	decreased resistance to an antimicrobial agent	Relative to: OMP_AN:27504 Genotype differences: +lacZ4787(del)( +acpP -lacZ4787(del) Condition differences: no differences	temperature:37C medium:LB agar medium:Novobiocin sodium salt	ECO:0007005	high throughput direct assay evidence used in manual assertion	PMID:21185072	towards some (CHEBI:31924) Novobiocin sodium salt	chemical concentration= 8 ug/ml normalized fitness= -4.55
OMP_AN:37151		OMP:0007879	decreased resistance to an antimicrobial agent	Relative to: OMP_AN:27565 Genotype differences: +lacZ4787(del)( +acpP -lacZ4787(del) Condition differences: no differences	temperature:37C medium:LB agar medium:Polymyxin B sulfate	ECO:0007005	high throughput direct assay evidence used in manual assertion	PMID:21185072	towards some (CHEBI:8310) Polymyxin B sulfate	chemical concentration= 6 ug/ml normalized fitness= -3.87
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References

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