OMP ST:71044 ! Escherichia coli K-12 BW25113 cydA::SPA-kan

From OMPwiki

Strain Summary

Strain Name

Escherichia coli K-12 BW25113 cydA::SPA-kan

Synonyms
Taxon Information
  • Pangenome: Escherichia coli
  • Subspecies and/or strain: K-12
  • NCBI Taxonomy ID: 83333
Genotype

DE(araD-araB)567 DE(rhaD-rhaB)568 F- Lambda- hsdR514 lacZ4787(del)(::rrnB-3) rph-1 cydA::SPA-kan

Strain Reference

PMID:21185072[1]

Strain availability
Ancestry

parent:OMP ST:800 ! Escherichia coli K-12 BW25113

Annotated phenotypes



Notes

Phenotype Annotation

Annotation ID Qualifier OMP ID OMP term name Relative phenotype information Experimental condition ECO ID ECO term name Reference Annotation Extension Notes

OMP_AN:37193

OMP:0007249

decreased resistance to a chemical

Relative to: OMP_AN:27594


Genotype differences:

+lacZ4787(del)( +cydA -lacZ4787(del)


Condition differences:

no differences

ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[1]

towards some (CHEBI:64758 + CHEBI:8984) SDS (CHEBI:8984) +EDTA (CHEBI:64758)

  • chemical concentration= 0.5%/0.5mM %/0.5mM
  • normalized fitness= -5

OMP_AN:37194

OMP:0007954

decreased population growth at high temperature

Relative to: OMP_AN:27621


Genotype differences:

+lacZ4787(del)( +cydA -lacZ4787(del)


Condition differences:
  • temperature:40C
  • medium37C
  • medium
  • temperature:40C
  • medium:LB agar
  • medium:High temperature
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[1]

  • chemical concentration= 40 C
  • normalized fitness= -6.19

OMP_AN:37195

OMP:0007249

decreased resistance to a chemical

Relative to: OMP_AN:27514


Genotype differences:

+lacZ4787(del)( +cydA -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Chlorpromazine hydrochloride
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[1]

towards some (CHEBI:3649) Chlorpromazine hydrochloride

  • chemical concentration= 6 uM
  • normalized fitness= -7.33

OMP_AN:37196

OMP:0007249

decreased resistance to a chemical

Relative to: OMP_AN:27501


Genotype differences:

+lacZ4787(del)( +cydA -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Dibucaine
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[1]

towards some (CHEBI:247956) Dibucaine

  • chemical concentration= 1.2 mM
  • normalized fitness= -4.49

OMP_AN:37197

OMP:0007912

decreased resistance to a chelator

Relative to: OMP_AN:27507


Genotype differences:

+lacZ4787(del)( +cydA -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:EDTA disodium salt dihydrate
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[1]

towards some (CHEBI:64758) EDTA disodium salt dihydrate

  • chemical concentration= 1 mM
  • normalized fitness= -4.43

OMP_AN:37198

OMP:0007879

decreased resistance to an antimicrobial agent

Relative to: OMP_AN:27550


Genotype differences:

+lacZ4787(del)( +cydA -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Ethidium Bromide
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[1]

towards some (CHEBI:4883) Ethidium Bromide

  • chemical concentration= 10 ug/ml
  • normalized fitness= -4.9

OMP_AN:37199

OMP:0007173

decreased resistance to host defense peptides

Relative to: OMP_AN:27563


Genotype differences:

+lacZ4787(del)( +cydA -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Indolicidin
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[1]

towards some (UniProtKB:P33046) Indolicidin

  • chemical concentration= 0.1 ug/ml
  • normalized fitness= -5.22

OMP_AN:37200

OMP:0007249

decreased resistance to a chemical

Relative to: OMP_AN:27593


Genotype differences:

+lacZ4787(del)( +cydA -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:(-)-Norepinephrine
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[1]

towards some (CHEBI:18357) (-)-Norepinephrine

  • chemical concentration= 100 uM
  • normalized fitness= -4.18

OMP_AN:37201

OMP:0007249

decreased resistance to a chemical

Relative to: OMP_AN:27570


Genotype differences:

+lacZ4787(del)( +cydA -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Propidium iodide
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[1]

towards some (CHEBI:51240) Propidium iodide

  • chemical concentration= 20 ug/ml
  • normalized fitness= -7.37

OMP_AN:37202

OMP:0007250

increased resistance to a chemical

Relative to: OMP_AN:27574


Genotype differences:

+lacZ4787(del)( +cydA -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Paraquat dichloride
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[1]

towards some (CHEBI:28786) Paraquat dichloride

  • chemical concentration= 18 uM
  • normalized fitness= 3.63

OMP_AN:37203

OMP:0007878

increased resistance to an antimicrobial agent

Relative to: OMP_AN:27553


Genotype differences:

+lacZ4787(del)( +cydA -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Puromycin dihydrochloride
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[1]

towards some (CHEBI:8642) Puromycin dihydrochloride

  • chemical concentration= 25 ug/ml
  • normalized fitness= 4.04

OMP_AN:37204

OMP:0007249

decreased resistance to a chemical

Relative to: OMP_AN:27568


Genotype differences:

+lacZ4787(del)( +cydA -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:(±)-Verapamil hydrochloride
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[1]

towards some (CHEBI:53188) (±)-Verapamil hydrochloride

  • chemical concentration= 1 mM
  • normalized fitness= -3.66

OMP_AN:37205

OMP:0006044

decreased resistance to beta-lactam

Relative to: OMP_AN:27532


Genotype differences:

+lacZ4787(del)( +cydA -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Aztreonam
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[1]

towards some (CHEBI:161680) Aztreonam

  • chemical concentration= 0.02 ug/ml
  • normalized fitness= -6.4

OMP_AN:37206

OMP:0007879

decreased resistance to an antimicrobial agent

Relative to: OMP_AN:27589


Genotype differences:

+lacZ4787(del)( +cydA -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Cefsulodin + Mecillinam
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[1]

towards some (CHEBI:31380 + CHEBI:51208) Cefsulodin + Mecillinam

  • chemical concentration= 6 + 0.03 ug/ml
  • normalized fitness= -7.76


Additional Information

Links

References

See Help:References for how to manage references in OMPwiki.

  1. 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 1.12 1.13 1.14 Nichols, RJ et al. (2011) Phenotypic landscape of a bacterial cell. Cell 144 143-56 PubMed OMPwiki page