OMP ST:71032 ! Escherichia coli K-12 BW25113 lpxA::SPA-kan

Strain Summary

Strain Name	Escherichia coli K-12 BW25113 lpxA::SPA-kan
Synonyms
Taxon Information	Pangenome: Escherichia coli Subspecies and/or strain: K-12 NCBI Taxonomy ID: 83333
Genotype	DE(araD-araB)567 DE(rhaD-rhaB)568 F- Lambda- hsdR514 lacZ4787(del)(::rrnB-3) rph-1 lpxA::SPA-kan
Strain Reference	PMID:21185072
Strain availability
Ancestry	parent:OMP ST:800 ! Escherichia coli K-12 BW25113
Annotated phenotypes
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Phenotype Annotation

Annotation ID	Qualifier	OMP ID	OMP term name	Relative phenotype information	Experimental condition	ECO ID	ECO term name	Reference	Annotation Extension	Notes
OMP_AN:37424		OMP:0007249	decreased resistance to a chemical	Relative to: OMP_AN:27571 Genotype differences: +lacZ4787(del)( +lpxA -lacZ4787(del) Condition differences: no differences	temperature:37C medium:LB agar medium:CCCP (Carbonyl cyanide 3-chlorophenylhydrazone)	ECO:0007005	high throughput direct assay evidence used in manual assertion	PMID:21185072	towards some (CHEBI:3259) CCCP (Carbonyl cyanide 3-chlorophenylhydrazone)	chemical concentration= 2 ug/ml normalized fitness= -3.78
OMP_AN:37425		OMP:0007879	decreased resistance to an antimicrobial agent	Relative to: OMP_AN:27549 Genotype differences: +lacZ4787(del)( +lpxA -lacZ4787(del) Condition differences: no differences	temperature:37C medium:LB agar medium:CHIR-090	ECO:0007005	high throughput direct assay evidence used in manual assertion	PMID:21185072	towards some (CHEBI:134107 CID:11546620) CHIR-090	chemical concentration= 0.025 ug/ml normalized fitness= -3.35
OMP_AN:37426		OMP:0007249	decreased resistance to a chemical	Relative to: OMP_AN:27514 Genotype differences: +lacZ4787(del)( +lpxA -lacZ4787(del) Condition differences: no differences	temperature:37C medium:LB agar medium:Chlorpromazine hydrochloride	ECO:0007005	high throughput direct assay evidence used in manual assertion	PMID:21185072	towards some (CHEBI:3649) Chlorpromazine hydrochloride	chemical concentration= 6 uM normalized fitness= -14.95
OMP_AN:37427		OMP:0006044	decreased resistance to beta-lactam	Relative to: OMP_AN:27537 Genotype differences: +lacZ4787(del)( +lpxA -lacZ4787(del) Condition differences: no differences	temperature:37C medium:LB agar medium:Mecillinam	ECO:0007005	high throughput direct assay evidence used in manual assertion	PMID:21185072	towards some (CHEBI:51208) Mecillinam	chemical concentration= 0.06 ug/ml normalized fitness= -5.99
OMP_AN:37428		OMP:0007250	increased resistance to a chemical	Relative to: OMP_AN:27573 Genotype differences: +lacZ4787(del)( +lpxA -lacZ4787(del) Condition differences: no differences	temperature:37C medium:LB agar medium:Procaine hydrochloride	ECO:0007005	high throughput direct assay evidence used in manual assertion	PMID:21185072	towards some (CHEBI:8431) Procaine hydrochloride	chemical concentration= 30 mM normalized fitness= 3.7
OMP_AN:37429		OMP:0006040	decreased resistance to quinolone	Relative to: OMP_AN:27543 Genotype differences: +lacZ4787(del)( +lpxA -lacZ4787(del) Condition differences: no differences	temperature:37C medium:LB agar medium:Streptonigrin	ECO:0007005	high throughput direct assay evidence used in manual assertion	PMID:21185072	towards some (CHEBI:9287) Streptonigrin	chemical concentration= 0.5 ug/ml normalized fitness= -7.44
OMP_AN:37430		OMP:0007879	decreased resistance to an antimicrobial agent	Relative to: OMP_AN:27588 Genotype differences: +lacZ4787(del)( +lpxA -lacZ4787(del) Condition differences: no differences	temperature:37C medium:LB agar medium:Triclosan/Irgasan	ECO:0007005	high throughput direct assay evidence used in manual assertion	PMID:21185072	towards some (CHEBI:164200) Triclosan/Irgasan	chemical concentration= 0.05 ug/ml normalized fitness= -6.41
OMP_AN:37431		OMP:0006045	increased resistance to beta-lactam compound	Relative to: OMP_AN:27532 Genotype differences: +lacZ4787(del)( +lpxA -lacZ4787(del) Condition differences: no differences	temperature:37C medium:LB agar medium:Aztreonam	ECO:0007005	high throughput direct assay evidence used in manual assertion	PMID:21185072	towards some (CHEBI:161680) Aztreonam	chemical concentration= 0.04 ug/ml normalized fitness= 3.77
OMP_AN:37432		OMP:0007879	decreased resistance to an antimicrobial agent	Relative to: OMP_AN:27560 Genotype differences: +lacZ4787(del)( +lpxA -lacZ4787(del) Condition differences: no differences	temperature:37C medium:LB agar medium:Bacitracin Zinc Sulfate	ECO:0007005	high throughput direct assay evidence used in manual assertion	PMID:21185072	towards some (CID:16219032) Bacitracin Zinc Sulfate	chemical concentration= 300 ug/ml normalized fitness= -4.91
OMP_AN:37433		OMP:0007250	increased resistance to a chemical	Relative to: OMP_AN:27569 Genotype differences: +lacZ4787(del)( +lpxA -lacZ4787(del) Condition differences: no differences	temperature:37C medium:LB agar medium:EtOH	ECO:0007005	high throughput direct assay evidence used in manual assertion	PMID:21185072	towards some (CHEBI:16236) EtOH	chemical concentration= 4 % normalized fitness= 3.77
OMP_AN:37434		OMP:0007520	increased population growth in high osmolarity	Relative to: OMP_AN:27595 Genotype differences: +lacZ4787(del)( +lpxA -lacZ4787(del) Condition differences: no differences	temperature:37C medium:LB agar medium:NaCl	ECO:0007005	high throughput direct assay evidence used in manual assertion	PMID:21185072	towards some (CHEBI:26710) NaCl	chemical concentration= 450 mM normalized fitness= 4.23
OMP_AN:37435		OMP:0006040	decreased resistance to quinolone	Relative to: OMP_AN:27541 Genotype differences: +lacZ4787(del)( +lpxA -lacZ4787(del) Condition differences: no differences	temperature:37C medium:LB agar medium:Nalidixic acid sodium salt	ECO:0007005	high throughput direct assay evidence used in manual assertion	PMID:21185072	towards some (CID:3864541) Nalidixic acid sodium salt	chemical concentration= 2 ug/ml normalized fitness= -3.92
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References

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