OMP ST:71025 ! Escherichia coli K-12 BW25113 frr::SPA-kan

Strain Summary

Strain Name	Escherichia coli K-12 BW25113 frr::SPA-kan
Synonyms
Taxon Information	Pangenome: Escherichia coli Subspecies and/or strain: K-12 NCBI Taxonomy ID: 83333
Genotype	DE(araD-araB)567 DE(rhaD-rhaB)568 F- Lambda- hsdR514 lacZ4787(del)(::rrnB-3) rph-1 frr::SPA-kan
Strain Reference	PMID:21185072
Strain availability
Ancestry	parent:OMP ST:800 ! Escherichia coli K-12 BW25113
Annotated phenotypes
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Phenotype Annotation

Annotation ID	Qualifier	OMP ID	OMP term name	Relative phenotype information	Experimental condition	ECO ID	ECO term name	Reference	Annotation Extension	Notes
OMP_AN:37254		OMP:0006036	decreased resistance to macrolide	Relative to: OMP_AN:27518 Genotype differences: +lacZ4787(del)( +frr -lacZ4787(del) Condition differences: no differences	temperature:37C medium:LB agar medium:Azithromycin	ECO:0007005	high throughput direct assay evidence used in manual assertion	PMID:21185072	towards some (CHEBI:2955) Azithromycin	chemical concentration= 1 ug/ml normalized fitness= -5.79
OMP_AN:37255		OMP:0007249	decreased resistance to a chemical	Relative to: OMP_AN:27514 Genotype differences: +lacZ4787(del)( +frr -lacZ4787(del) Condition differences: no differences	temperature:37C medium:LB agar medium:Chlorpromazine hydrochloride	ECO:0007005	high throughput direct assay evidence used in manual assertion	PMID:21185072	towards some (CHEBI:3649) Chlorpromazine hydrochloride	chemical concentration= 6 uM normalized fitness= -11.84
OMP_AN:37256		OMP:0007879	decreased resistance to an antimicrobial agent	Relative to: OMP_AN:27550 Genotype differences: +lacZ4787(del)( +frr -lacZ4787(del) Condition differences: no differences	temperature:37C medium:LB agar medium:Ethidium Bromide	ECO:0007005	high throughput direct assay evidence used in manual assertion	PMID:21185072	towards some (CHEBI:4883) Ethidium Bromide	chemical concentration= 50 ug/ml normalized fitness= -3.67
OMP_AN:37257		OMP:0007903	decreased resistance to bile acids	Relative to: OMP_AN:27584 Genotype differences: +lacZ4787(del)( +frr -lacZ4787(del) Condition differences: no differences	temperature:37C medium:LB agar medium:Taurocholic acid sodium salt hydrate	ECO:0007005	high throughput direct assay evidence used in manual assertion	PMID:21185072	towards some (CID:23687511) Taurocholic acid sodium salt hydrate	chemical concentration= 1 % normalized fitness= -4.87
OMP_AN:37258		OMP:0007074	increased growth factor requirement	Relative to: OMP_AN:27598 Genotype differences: +lacZ4787(del)( +frr -lacZ4787(del) Condition differences: no differences	temperature:37C medium:MOPS medium:Iron excess-FeSO4 heptahydride	ECO:0007005	high throughput direct assay evidence used in manual assertion	PMID:21185072	towards some (CHEBI:75836) Iron excess-FeSO4 heptahydride	chemical concentration= 1mM (MOPS; normal 100uM) normalized fitness= 3.71
OMP_AN:37259		OMP:0007888	increased resistance to a metal	Relative to: OMP_AN:27608 Genotype differences: +lacZ4787(del)( +frr -lacZ4787(del) Condition differences: no differences	temperature:37C medium:MOPS medium:Iron excess-FeSO4 heptahydride	ECO:0007005	high throughput direct assay evidence used in manual assertion	PMID:21185072	towards some (CHEBI:75836) Iron excess-FeSO4 heptahydride	chemical concentration= 1mM (MOPS; normal 100uM) normalized fitness= 3.71
OMP_AN:37260		OMP:0007074	increased growth factor requirement	Relative to: OMP_AN:27599 Genotype differences: +lacZ4787(del)( +frr -lacZ4787(del) Condition differences: no differences	temperature:37C medium:MOPS medium:Iron starvation-FeSO4·7H2O	ECO:0007005	high throughput direct assay evidence used in manual assertion	PMID:21185072	towards some (CHEBI:75836) Iron starvation-FeSO4·7H2O	chemical concentration= 2uM (MOPS; normal 100uM) normalized fitness= 3.45
OMP_AN:37261		OMP:0007936	increased resistance to nutrient limitation	Relative to: OMP_AN:27609 Genotype differences: +lacZ4787(del)( +frr -lacZ4787(del) Condition differences: no differences	temperature:37C medium:MOPS medium:Iron starvation-FeSO4·7H2O	ECO:0007005	high throughput direct assay evidence used in manual assertion	PMID:21185072	towards some (CHEBI:75836) Iron starvation-FeSO4·7H2O	chemical concentration= 2uM (MOPS; normal 100uM) normalized fitness= 3.45
OMP_AN:37262		OMP:0006036	decreased resistance to macrolide	Relative to: OMP_AN:27521 Genotype differences: +lacZ4787(del)( +frr -lacZ4787(del) Condition differences: no differences	temperature:37C medium:LB agar medium:Spiramycin	ECO:0007005	high throughput direct assay evidence used in manual assertion	PMID:21185072	towards some (CID:6419898) Spiramycin	chemical concentration= 5 ug/ml normalized fitness= -6.81
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References

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