OMP ST:71018 ! Escherichia coli K-12 BW25113 lpxC(G210S)-kan

Strain Summary

Strain Name	Escherichia coli K-12 BW25113 lpxC(G210S)-kan
Synonyms	ECK0097-B (Nichols et al. 2011. Supplemental Table S2)
Taxon Information	Pangenome: Escherichia coli Subspecies and/or strain: K-12 NCBI Taxonomy ID: 83333
Genotype	DE(araD-araB)567 DE(rhaD-rhaB)568 F- Lambda- hsdR514 lacZ4787(del)(::rrnB-3) rph-1 lpxC(G210S)-kan
Strain Reference	PMID:21185072
Strain availability
Ancestry	parent:OMP ST:800 ! Escherichia coli K-12 BW25113
Annotated phenotypes
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Phenotype Annotation

Annotation ID	Qualifier	OMP ID	OMP term name	Relative phenotype information	Experimental condition	ECO ID	ECO term name	Reference	Annotation Extension	Notes
OMP_AN:37442		OMP:0007249	decreased resistance to a chemical	Relative to: OMP_AN:27571 Genotype differences: +lacZ4787(del)( +lpxC(G210S)-kan -lacZ4787(del) Condition differences: no differences	temperature:37C medium:LB agar medium:CCCP (Carbonyl cyanide 3-chlorophenylhydrazone)	ECO:0007005	high throughput direct assay evidence used in manual assertion	PMID:21185072	towards some (CHEBI:3259) CCCP (Carbonyl cyanide 3-chlorophenylhydrazone)	chemical concentration= 2 ug/ml normalized fitness= -10.94
OMP_AN:37443		OMP:0007249	decreased resistance to a chemical	Relative to: OMP_AN:27514 Genotype differences: +lacZ4787(del)( +lpxC(G210S)-kan -lacZ4787(del) Condition differences: no differences	temperature:37C medium:LB agar medium:Chlorpromazine hydrochloride	ECO:0007005	high throughput direct assay evidence used in manual assertion	PMID:21185072	towards some (CHEBI:3649) Chlorpromazine hydrochloride	chemical concentration= 6 uM normalized fitness= -18.51
OMP_AN:37444		OMP:0007903	decreased resistance to bile acids	Relative to: OMP_AN:27585 Genotype differences: +lacZ4787(del)( +lpxC(G210S)-kan -lacZ4787(del) Condition differences: no differences	temperature:37C medium:LB agar medium:Cholate sodium hydrate	ECO:0007005	high throughput direct assay evidence used in manual assertion	PMID:21185072	towards some (CID:23679061) Cholate sodium hydrate	chemical concentration= 2 % normalized fitness= -3.21
OMP_AN:37445		OMP:0007250	increased resistance to a chemical	Relative to: OMP_AN:27511 Genotype differences: +lacZ4787(del)( +lpxC(G210S)-kan -lacZ4787(del) Condition differences: no differences	temperature:37C medium:LB agar medium:(-)-Epinephrine	ECO:0007005	high throughput direct assay evidence used in manual assertion	PMID:21185072	towards some (CHEBI:28918) (-)-Epinephrine	chemical concentration= 50 uM normalized fitness= 3.63
OMP_AN:37446		OMP:0007879	decreased resistance to an antimicrobial agent	Relative to: OMP_AN:27550 Genotype differences: +lacZ4787(del)( +lpxC(G210S)-kan -lacZ4787(del) Condition differences: no differences	temperature:37C medium:LB agar medium:Ethidium Bromide	ECO:0007005	high throughput direct assay evidence used in manual assertion	PMID:21185072	towards some (CHEBI:4883) Ethidium Bromide	chemical concentration= 50 ug/ml normalized fitness= -6.28
OMP_AN:37447		OMP:0007249	decreased resistance to a chemical	Relative to: OMP_AN:27570 Genotype differences: +lacZ4787(del)( +lpxC(G210S)-kan -lacZ4787(del) Condition differences: no differences	temperature:37C medium:LB agar medium:Propidium iodide	ECO:0007005	high throughput direct assay evidence used in manual assertion	PMID:21185072	towards some (CHEBI:51240) Propidium iodide	chemical concentration= 20 ug/ml normalized fitness= -3.69
OMP_AN:37448		OMP:0007249	decreased resistance to a chemical	Relative to: OMP_AN:27513 Genotype differences: +lacZ4787(del)( +lpxC(G210S)-kan -lacZ4787(del) Condition differences: no differences	temperature:37C medium:LB agar medium:Phenazine methosulfate (PMS)	ECO:0007005	high throughput direct assay evidence used in manual assertion	PMID:21185072	towards some (CHEBI:8055) Phenazine methosulfate (PMS)	chemical concentration= 0.1 mM normalized fitness= -3.4
OMP_AN:37449		OMP:0007879	decreased resistance to an antimicrobial agent	Relative to: OMP_AN:27564 Genotype differences: +lacZ4787(del)( +lpxC(G210S)-kan -lacZ4787(del) Condition differences: no differences	temperature:37C medium:LB agar medium:Phleomycin	ECO:0007005	high throughput direct assay evidence used in manual assertion	PMID:21185072	towards some (CHEBI:75044) Phleomycin	chemical concentration= 1 ug/ml normalized fitness= -3.5
OMP_AN:37450		OMP:0007879	decreased resistance to an antimicrobial agent	Relative to: OMP_AN:27546 Genotype differences: +lacZ4787(del)( +lpxC(G210S)-kan -lacZ4787(del) Condition differences: no differences	temperature:37C medium:LB agar medium:Pyocyanin	ECO:0007005	high throughput direct assay evidence used in manual assertion	PMID:21185072	towards some (CHEBI:8653) Pyocyanin	chemical concentration= 10 ug/ml normalized fitness= -3.5
OMP_AN:37451		OMP:0006040	decreased resistance to quinolone	Relative to: OMP_AN:27543 Genotype differences: +lacZ4787(del)( +lpxC(G210S)-kan -lacZ4787(del) Condition differences: no differences	temperature:37C medium:LB agar medium:Streptonigrin	ECO:0007005	high throughput direct assay evidence used in manual assertion	PMID:21185072	towards some (CHEBI:9287) Streptonigrin	chemical concentration= 0.5 ug/ml normalized fitness= -9.56
OMP_AN:37452		OMP:0007879	decreased resistance to an antimicrobial agent	Relative to: OMP_AN:27588 Genotype differences: +lacZ4787(del)( +lpxC(G210S)-kan -lacZ4787(del) Condition differences: no differences	temperature:37C medium:LB agar medium:Triclosan/Irgasan	ECO:0007005	high throughput direct assay evidence used in manual assertion	PMID:21185072	towards some (CHEBI:164200) Triclosan/Irgasan	chemical concentration= 0.05 ug/ml normalized fitness= -14.3
OMP_AN:37453		OMP:0007249	decreased resistance to a chemical	Relative to: OMP_AN:27569 Genotype differences: +lacZ4787(del)( +lpxC(G210S)-kan -lacZ4787(del) Condition differences: no differences	temperature:37C medium:LB agar medium:EtOH	ECO:0007005	high throughput direct assay evidence used in manual assertion	PMID:21185072	towards some (CHEBI:16236) EtOH	chemical concentration= 6 % normalized fitness= -3.26
OMP_AN:37454		OMP:0007520	increased population growth in high osmolarity	Relative to: OMP_AN:27595 Genotype differences: +lacZ4787(del)( +lpxC(G210S)-kan -lacZ4787(del) Condition differences: no differences	temperature:37C medium:LB agar medium:NaCl	ECO:0007005	high throughput direct assay evidence used in manual assertion	PMID:21185072	towards some (CHEBI:26710) NaCl	chemical concentration= 450 mM normalized fitness= 3.77
OMP_AN:37455		OMP:0007008	decreased population growth at acidic pH	Relative to: OMP_AN:27622 Genotype differences: +lacZ4787(del)( +lpxC(G210S)-kan -lacZ4787(del) Condition differences: no differences	temperature:37C medium:LB agar medium:MES-HOMOPIPES	ECO:0007005	high throughput direct assay evidence used in manual assertion	PMID:21185072	towards some (**SID:329817955 SID:57651422) MES-HOMOPIPES	chemical concentration= pH 4 normalized fitness= -3.75
OMP_AN:37456		OMP:0007009	increased population growth at acidic pH	Relative to: OMP_AN:27622 Genotype differences: +lacZ4787(del)( +lpxC(G210S)-kan -lacZ4787(del) Condition differences: no differences	temperature:37C medium:LB agar medium:MES-HOMOPIPES	ECO:0007005	high throughput direct assay evidence used in manual assertion	PMID:21185072	towards some (**SID:329817955 SID:57651422) MES-HOMOPIPES	chemical concentration= 6 normalized fitness= 3.82
OMP_AN:37457		OMP:0007879	decreased resistance to an antimicrobial agent	Relative to: OMP_AN:27551 Genotype differences: +lacZ4787(del)( +lpxC(G210S)-kan -lacZ4787(del) Condition differences: no differences	temperature:37C medium:LB agar medium:Rifampicin	ECO:0007005	high throughput direct assay evidence used in manual assertion	PMID:21185072	towards some (CHEBI:28077) Rifampicin	chemical concentration= 2 ug/ml normalized fitness= -8.14
OMP_AN:37458		OMP:0006057	decreased resistance to aminoglycoside	Relative to: OMP_AN:27527 Genotype differences: +lacZ4787(del)( +lpxC(G210S)-kan -lacZ4787(del) Condition differences: no differences	temperature:37C medium:LB agar medium:Spectinomycin dihydrochloride pentahydrate	ECO:0007005	high throughput direct assay evidence used in manual assertion	PMID:21185072	towards some (CHEBI:9217) Spectinomycin dihydrochloride pentahydrate	chemical concentration= 4 ug/ml normalized fitness= -5.27
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References

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