OMP ST:6127 ! Escherichia coli K-12 JW3710

From OMPwiki

Strain Summary

Strain Name

Escherichia coli K-12 JW3710

Synonyms

JW3710-1

Taxon Information
  • Pangenome: Escherichia coli
  • Subspecies and/or strain: K-12
  • NCBI Taxonomy ID: 83333
Genotype

DE(araD-araB)567 DE(rhaD-rhaB)568 F- Lambda- hsdR514 lacZ4787(del)(::rrnB-3) rph-1 atpD761(del)::kan

Strain Reference

Coli Genetic Stock Center

Strain availability

Coli Genetic Stock Center

Ancestry

parent:OMP ST:800 ! Escherichia coli K-12 BW25113

Annotated phenotypes



Notes

Phenotype Annotation

Annotation ID Qualifier OMP ID OMP term name Relative phenotype information Experimental condition ECO ID ECO term name Reference Annotation Extension Notes

OMP_AN:1192

OMP:0007092

decreased cell motility by swarming

Relative to: OMP_AN:881


Genotype differences:

+DE(araD-araB)567 +DE(rhaD-rhaB)568 +hsdR514 +lacZ4787(del)( +atpD761(del) -IN(rrnD-rrnE)1


Condition differences:

no differences

  • temperature:33C
  • medium:swarming motility medium
ECO:0005803

motility assay evidence

PMID:17122336[1]

OMP_AN:1193

OMP:0007093

decreased cell motility by swimming

Relative to: OMP_AN:882


Genotype differences:

+DE(araD-araB)567 +DE(rhaD-rhaB)568 +hsdR514 +lacZ4787(del)( +atpD761(del) -IN(rrnD-rrnE)1


Condition differences:

no differences

  • temperature:33C
  • medium:swimming motility medium
ECO:0005803

motility assay evidence

PMID:17122336[1]

OMP_AN:34059

OMP:0007249

decreased resistance to a chemical

Relative to: OMP_AN:27594


Genotype differences:

+lacZ4787(del)( +atpD761(del) -lacZ4787(del)


Condition differences:

no differences

ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:64758 + CHEBI:8984) SDS (CHEBI:8984) +EDTA (CHEBI:64758)

  • chemical concentration= 0.5%/0.5mM %/0.5mM
  • normalized fitness= -6.58

OMP_AN:34060

OMP:0007879

decreased resistance to an antimicrobial agent

Relative to: OMP_AN:27557


Genotype differences:

+lacZ4787(del)( +atpD761(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:A22
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CID:2830968) A22

  • chemical concentration= 5 ug/ml
  • normalized fitness= -3.35

OMP_AN:34061

OMP:0007075

decreased growth factor requirement

Relative to: OMP_AN:27601


Genotype differences:

+lacZ4787(del)( +atpD761(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:M9
  • medium:Sodium Acetate anhydrous
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:32954) Sodium Acetate anhydrous

  • chemical concentration= 0.6%
  • normalized fitness= -4.64

OMP_AN:34062

OMP:0007070

decreased organic carbon source utilization

Relative to: OMP_AN:27611


Genotype differences:

+lacZ4787(del)( +atpD761(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:M9
  • medium:Sodium Acetate anhydrous
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:32954) Sodium Acetate anhydrous

  • chemical concentration= 0.6%
  • normalized fitness= -4.64

OMP_AN:34063

OMP:0006044

decreased resistance to beta-lactam

Relative to: OMP_AN:27531


Genotype differences:

+lacZ4787(del)( +atpD761(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Ampicillin
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:28971) Ampicillin

  • chemical concentration= 2 ug/ml
  • normalized fitness= -4.32

OMP_AN:34064

OMP:0007879

decreased resistance to an antimicrobial agent

Relative to: OMP_AN:27552


Genotype differences:

+lacZ4787(del)( +atpD761(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Azidothymidine
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:10110) Azidothymidine

  • chemical concentration= 0.5 ng/ml
  • normalized fitness= -4.11

OMP_AN:34065

OMP:0007896

decreased resistance to a surfactant

Relative to: OMP_AN:27581


Genotype differences:

+lacZ4787(del)( +atpD761(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Benzalkonium chloride
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:3020) Benzalkonium chloride

  • chemical concentration= 10 ug/ml
  • normalized fitness= -4.04

OMP_AN:34066

OMP:0006044

decreased resistance to beta-lactam

Relative to: OMP_AN:27534


Genotype differences:

+lacZ4787(del)( +atpD761(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Cefoxitin sodium hydrate
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:3501) Cefoxitin sodium hydrate

  • chemical concentration= 1 ug/ml
  • normalized fitness= -4.8

OMP_AN:34067

OMP:0007249

decreased resistance to a chemical

Relative to: OMP_AN:27514


Genotype differences:

+lacZ4787(del)( +atpD761(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Chlorpromazine hydrochloride
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:3649) Chlorpromazine hydrochloride

  • chemical concentration= 6 uM
  • normalized fitness= -4.19

OMP_AN:34068

OMP:0007903

decreased resistance to bile acids

Relative to: OMP_AN:27585


Genotype differences:

+lacZ4787(del)( +atpD761(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Cholate sodium hydrate
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CID:23679061) Cholate sodium hydrate

  • chemical concentration= 0.1 %
  • normalized fitness= -5.79

OMP_AN:34069

OMP:0007903

decreased resistance to bile acids

Relative to: OMP_AN:27586


Genotype differences:

+lacZ4787(del)( +atpD761(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Deoxycholate sodium
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:9177) Deoxycholate sodium

  • chemical concentration= 2 %
  • normalized fitness= -4.14

OMP_AN:34070

OMP:0007249

decreased resistance to a chemical

Relative to: OMP_AN:27501


Genotype differences:

+lacZ4787(del)( +atpD761(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Dibucaine
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:247956) Dibucaine

  • chemical concentration= 0.4 mM
  • normalized fitness= -3.62

OMP_AN:34071

OMP:0007879

decreased resistance to an antimicrobial agent

Relative to: OMP_AN:27590


Genotype differences:

+lacZ4787(del)( +atpD761(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Fosfomycin (CID:6093162) +Glucose-6-phosphate (CHEBI:4170)
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CID:6093162 + CHEBI:4170) Fosfomycin (CID:6093162) +Glucose-6-phosphate (CHEBI:4170)

  • chemical concentration= 0.2 ug/ml (in 50 mg/ml G6P)
  • normalized fitness= -3.5

OMP_AN:34072

OMP:0007879

decreased resistance to an antimicrobial agent

Relative to: OMP_AN:27503


Genotype differences:

+lacZ4787(del)( +atpD761(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Nitrofurantoin
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:71415) Nitrofurantoin

  • chemical concentration= 1.5 ug/ml
  • normalized fitness= -3.61

OMP_AN:34073

OMP:0007249

decreased resistance to a chemical

Relative to: OMP_AN:27593


Genotype differences:

+lacZ4787(del)( +atpD761(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:(-)-Norepinephrine
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:18357) (-)-Norepinephrine

  • chemical concentration= 1000 uM
  • normalized fitness= -4.66

OMP_AN:34074

OMP:0006057

decreased resistance to aminoglycoside

Relative to: OMP_AN:27528


Genotype differences:

+lacZ4787(del)( +atpD761(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Streptomycin sulfate
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:32158) Streptomycin sulfate

  • chemical concentration= 0.05 ug/ml
  • normalized fitness= -3.56

OMP_AN:34075

OMP:0007075

decreased growth factor requirement

Relative to: OMP_AN:27607


Genotype differences:

+lacZ4787(del)( +atpD761(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:M9
  • medium:Succinic acid
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:15741) Succinic acid

  • chemical concentration= 0.3%
  • normalized fitness= -6.47

OMP_AN:34076

OMP:0007070

decreased organic carbon source utilization

Relative to: OMP_AN:27617


Genotype differences:

+lacZ4787(del)( +atpD761(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:M9
  • medium:Succinic acid
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:15741) Succinic acid

  • chemical concentration= 0.3%
  • normalized fitness= -6.47

OMP_AN:34077

OMP:0007896

decreased resistance to a surfactant

Relative to: OMP_AN:27583


Genotype differences:

+lacZ4787(del)( +atpD761(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Triton X-100
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:9750) Triton X-100

  • chemical concentration= 0.01 %
  • normalized fitness= -3.81

OMP_AN:34078

OMP:0007879

decreased resistance to an antimicrobial agent

Relative to: OMP_AN:27554


Genotype differences:

+lacZ4787(del)( +atpD761(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Tunicamycin
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:29699) Tunicamycin

  • chemical concentration= 7.5 ug/ml
  • normalized fitness= -8.36

OMP_AN:34079

OMP:0006045

increased resistance to beta-lactam compound

Relative to: OMP_AN:27532


Genotype differences:

+lacZ4787(del)( +atpD761(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Aztreonam
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:161680) Aztreonam

  • chemical concentration= 0.04 ug/ml
  • normalized fitness= 4.44

OMP_AN:34080

OMP:0006044

decreased resistance to beta-lactam

Relative to: OMP_AN:27596


Genotype differences:

+lacZ4787(del)( +atpD761(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Cefaclor
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:3478) Cefaclor

  • chemical concentration= 3 ug/ml
  • normalized fitness= -4.75

OMP_AN:34081

OMP:0006040

decreased resistance to quinolone

Relative to: OMP_AN:27542


Genotype differences:

+lacZ4787(del)( +atpD761(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Norfloxacin
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:100246) Norfloxacin

  • chemical concentration= 0.01 ug/ml
  • normalized fitness= -6.46


Additional Information

Links

References

See Help:References for how to manage references in OMPwiki.

  1. 1.0 1.1 Inoue, T et al. (2007) Genome-wide screening of genes required for swarming motility in Escherichia coli K-12. J. Bacteriol. 189 950-7 PubMed OMPwiki page
  2. 2.00 2.01 2.02 2.03 2.04 2.05 2.06 2.07 2.08 2.09 2.10 2.11 2.12 2.13 2.14 2.15 2.16 2.17 2.18 2.19 2.20 2.21 2.22 Nichols, RJ et al. (2011) Phenotypic landscape of a bacterial cell. Cell 144 143-56 PubMed OMPwiki page