OMP ST:1287 ! Escherichia coli K-12 JW3587

From OMPwiki

Strain Summary

Strain Name

Escherichia coli K-12 JW3587

Synonyms

JW3587-1

Taxon Information
  • Pangenome: Escherichia coli
  • Subspecies and/or strain: K-12
  • NCBI Taxonomy ID: 83333
Genotype

DE(araD-araB)567 DE(rhaD-rhaB)568 F- Lambda- hsdR514 lacZ4787(del)(::rrnB-3) rph-1 gpmM724(del)::kan

Strain Reference

Coli Genetic Stock Center

Strain availability

Coli Genetic Stock Center

Ancestry

parent:OMP ST:800 ! Escherichia coli K-12 BW25113

Annotated phenotypes



Notes

Gene gpmM referred to by its old name (gpmI) in PMID:17122336

Phenotype Annotation

Annotation ID Qualifier OMP ID OMP term name Relative phenotype information Experimental condition ECO ID ECO term name Reference Annotation Extension Notes

OMP_AN:1124

OMP:0007092

decreased cell motility by swarming

Relative to: OMP_AN:881


Genotype differences:

+DE(araD-araB)567 +DE(rhaD-rhaB)568 +hsdR514 +lacZ4787(del)( +gpmM724(del) -IN(rrnD-rrnE)1


Condition differences:

no differences

  • temperature:33C
  • medium:swarming motility medium
ECO:0005803

motility assay evidence

PMID:17122336[1]

OMP_AN:33739

OMP:0007879

decreased resistance to an antimicrobial agent

Relative to: OMP_AN:27559


Genotype differences:

+lacZ4787(del)( +gpmM724(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Actinomycin D
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:27666 CID:457193) Actinomycin D

  • chemical concentration= 5 ug/ml
  • normalized fitness= -3.53

OMP_AN:33740

OMP:0007895

increased resistance to a surfactant

Relative to: OMP_AN:27581


Genotype differences:

+lacZ4787(del)( +gpmM724(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Benzalkonium chloride
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:3020) Benzalkonium chloride

  • chemical concentration= 1 ug/ml
  • normalized fitness= 3.55

OMP_AN:33741

OMP:0007879

decreased resistance to an antimicrobial agent

Relative to: OMP_AN:27561


Genotype differences:

+lacZ4787(del)( +gpmM724(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Bleomycin sulfate
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:34582) Bleomycin sulfate

  • chemical concentration= 0.1 ug/ml
  • normalized fitness= -3.92

OMP_AN:33742

OMP:0007888

increased resistance to a metal

Relative to: OMP_AN:27579


Genotype differences:

+lacZ4787(del)( +gpmM724(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Copper stress-CuCl2 dihydrate
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:86318) Copper stress-CuCl2 dihydrate

  • chemical concentration= 2 mM (LB)
  • normalized fitness= 3.56

OMP_AN:33743

OMP:0007903

decreased resistance to bile acids

Relative to: OMP_AN:27586


Genotype differences:

+lacZ4787(del)( +gpmM724(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Deoxycholate sodium
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:9177) Deoxycholate sodium

  • chemical concentration= 2 %
  • normalized fitness= -3.57

OMP_AN:33744

OMP:0007912

decreased resistance to a chelator

Relative to: OMP_AN:27507


Genotype differences:

+lacZ4787(del)( +gpmM724(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:EDTA disodium salt dihydrate
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:64758) EDTA disodium salt dihydrate

  • chemical concentration= 1 mM
  • normalized fitness= -5.33

OMP_AN:33745

OMP:0007250

increased resistance to a chemical

Relative to: OMP_AN:27572


Genotype differences:

+lacZ4787(del)( +gpmM724(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Epigallocatechin gallate (EGCG)
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:4806) Epigallocatechin gallate (EGCG)

  • chemical concentration= 50 uM
  • normalized fitness= 3.57

OMP_AN:33746

OMP:0007250

increased resistance to a chemical

Relative to: OMP_AN:27513


Genotype differences:

+lacZ4787(del)( +gpmM724(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Phenazine methosulfate (PMS)
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:8055) Phenazine methosulfate (PMS)

  • chemical concentration= 0.02 mM
  • normalized fitness= 3.42

OMP_AN:33747

OMP:0007879

decreased resistance to an antimicrobial agent

Relative to: OMP_AN:27564


Genotype differences:

+lacZ4787(del)( +gpmM724(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Phleomycin
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:75044) Phleomycin

  • chemical concentration= 1 ug/ml
  • normalized fitness= -4.02

OMP_AN:33748

OMP:0006057

decreased resistance to aminoglycoside

Relative to: OMP_AN:27528


Genotype differences:

+lacZ4787(del)( +gpmM724(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Streptomycin sulfate
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:32158) Streptomycin sulfate

  • chemical concentration= 0.05 ug/ml
  • normalized fitness= -3.67

OMP_AN:33749

OMP:0006040

decreased resistance to quinolone

Relative to: OMP_AN:27543


Genotype differences:

+lacZ4787(del)( +gpmM724(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Streptonigrin
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:9287) Streptonigrin

  • chemical concentration= 0.5 ug/ml
  • normalized fitness= -3.29

OMP_AN:33750

OMP:0007249

decreased resistance to a chemical

Relative to: OMP_AN:27567


Genotype differences:

+lacZ4787(del)( +gpmM724(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Theophylline anhydrous
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:28177) Theophylline anhydrous

  • chemical concentration= 100 ug/ml
  • normalized fitness= -4.57

OMP_AN:33751

OMP:0007249

decreased resistance to a chemical

Relative to: OMP_AN:27569


Genotype differences:

+lacZ4787(del)( +gpmM724(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:EtOH
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:16236) EtOH

  • chemical concentration= 6 %
  • normalized fitness= -3.78

OMP_AN:33752

OMP:0007520

increased population growth in high osmolarity

Relative to: OMP_AN:27595


Genotype differences:

+lacZ4787(del)( +gpmM724(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:NaCl
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:26710) NaCl

  • chemical concentration= 150 mM
  • normalized fitness= 4.41

OMP_AN:33753

OMP:0006040

decreased resistance to quinolone

Relative to: OMP_AN:27542


Genotype differences:

+lacZ4787(del)( +gpmM724(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Norfloxacin
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:100246) Norfloxacin

  • chemical concentration= 0.04 ug/ml
  • normalized fitness= -3.42


Additional Information

Links

References

See Help:References for how to manage references in OMPwiki.

  1. Inoue, T et al. (2007) Genome-wide screening of genes required for swarming motility in Escherichia coli K-12. J. Bacteriol. 189 950-7 PubMed OMPwiki page
  2. 2.00 2.01 2.02 2.03 2.04 2.05 2.06 2.07 2.08 2.09 2.10 2.11 2.12 2.13 2.14 Nichols, RJ et al. (2011) Phenotypic landscape of a bacterial cell. Cell 144 143-56 PubMed OMPwiki page