OMP ST:1130 ! Escherichia coli K-12 JW2866

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Strain Summary

Strain Name

Escherichia coli K-12 JW2866

Synonyms

JW2866-3

Taxon Information
  • Pangenome: Escherichia coli
  • Subspecies and/or strain: K-12
  • NCBI Taxonomy ID: 83333
Genotype

DE(araD-araB)567 DE(rhaD-rhaB)568 F- Lambda- hsdR514 lacZ4787(del)(::rrnB-3) rph-1 ygfZ749(del)::kan

Strain Reference

Coli Genetic Stock Center

Strain availability

Coli Genetic Stock Center

Ancestry

parent:OMP ST:800 ! Escherichia coli K-12 BW25113

Annotated phenotypes


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Notes

Phenotype Annotation

Annotation ID Qualifier OMP ID OMP term name Relative phenotype information Experimental condition ECO ID ECO term name Reference Annotation Extension Notes

OMP_AN:1046

OMP:0007092

decreased cell motility by swarming

Relative to: OMP_AN:881

Genotype differences:

+DE(araD-araB)567 +DE(rhaD-rhaB)568 +hsdR514 +lacZ4787(del)( +ygfZ749(del) -IN(rrnD-rrnE)1

Condition differences:

no differences

  • temperature:33C
  • medium:swarming motility medium
 ECO:0005803

motility assay evidence

PMID:17122336[1]

OMP_AN:32674

OMP:0007249

decreased resistance to a chemical

Relative to: OMP_AN:27575

Genotype differences:

+lacZ4787(del)( +ygfZ749(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Acriflavine (acriflavinium chloride)
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:74728) Acriflavine (acriflavinium chloride)

  • chemical concentration= 10 ug/ml
  • normalized fitness= -3.82

OMP_AN:32675

OMP:0006036

decreased resistance to macrolide

Relative to: OMP_AN:27518

Genotype differences:

+lacZ4787(del)( +ygfZ749(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Azithromycin
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:2955) Azithromycin

  • chemical concentration= 0.1 ug/ml
  • normalized fitness= -4.44

OMP_AN:32676

OMP:0007249

decreased resistance to a chemical

Relative to: OMP_AN:27510

Genotype differences:

+lacZ4787(del)( +ygfZ749(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Mitomycin C
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:27504) Mitomycin C

  • chemical concentration= 0.1 ug/ml
  • normalized fitness= -4.7

OMP_AN:32677

OMP:0007886

decreased resistance to a metal

Relative to: OMP_AN:27580

Genotype differences:

+lacZ4787(del)( +ygfZ749(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Nickel stress-Ni(II)Cl2 hexahydrate
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:53542) Nickel stress-Ni(II)Cl2 hexahydrate

  • chemical concentration= 0.1 mM (LB)
  • normalized fitness= -4.15

OMP_AN:32678

OMP:0007896

decreased resistance to a surfactant

Relative to: OMP_AN:27582

Genotype differences:

+lacZ4787(del)( +ygfZ749(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:SDS
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:8984) SDS

  • chemical concentration= 0.5 %
  • normalized fitness= -4.36

OMP_AN:32679

OMP:0007249

decreased resistance to a chemical

Relative to: OMP_AN:27567

Genotype differences:

+lacZ4787(del)( +ygfZ749(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Theophylline anhydrous
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:28177) Theophylline anhydrous

  • chemical concentration= 100 ug/ml
  • normalized fitness= -3.55

OMP_AN:32680

OMP:0007879

decreased resistance to an antimicrobial agent

Relative to: OMP_AN:27504

Genotype differences:

+lacZ4787(del)( +ygfZ749(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Novobiocin sodium salt
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:31924) Novobiocin sodium salt

  • chemical concentration= 8 ug/ml
  • normalized fitness= -3.82
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Additional Information

Links

References

See Help:References for how to manage references in OMPwiki.

  1. ↑ Inoue, T et al. (2007) Genome-wide screening of genes required for swarming motility in Escherichia coli K-12. J. Bacteriol. 189 950-7 PubMed OMPwiki page
  2. ↑ 2.0 2.1 2.2 2.3 2.4 2.5 2.6 Nichols, RJ et al. (2011) Phenotypic landscape of a bacterial cell. Cell 144 143-56 PubMed OMPwiki page
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