OMP ST:1089 ! Escherichia coli K-12 JW2409

Strain Summary

Strain Name	Escherichia coli K-12 JW2409
Synonyms	JW2409-1
Taxon Information	Pangenome: Escherichia coli Subspecies and/or strain: K-12 NCBI Taxonomy ID: 83333
Genotype	DE(araD-araB)567 DE(rhaD-rhaB)568 F- Lambda- hsdR514 lacZ4787(del)(::rrnB-3) rph-1 ptsI745(del)::kan
Strain Reference	Coli Genetic Stock Center
Strain availability	Coli Genetic Stock Center
Ancestry	parent:OMP ST:800 ! Escherichia coli K-12 BW25113
Annotated phenotypes
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Phenotype Annotation

Annotation ID	Qualifier	OMP ID	OMP term name	Relative phenotype information	Experimental condition	ECO ID	ECO term name	Reference	Annotation Extension	Notes
OMP_AN:1001		OMP:0007092	decreased cell motility by swarming	Relative to: OMP_AN:881 Genotype differences: +DE(araD-araB)567 +DE(rhaD-rhaB)568 +hsdR514 +lacZ4787(del)( +ptsI745(del) -IN(rrnD-rrnE)1 Condition differences: no differences	temperature:33C medium:swarming motility medium	ECO:0005803	motility assay evidence	PMID:17122336
OMP_AN:31668		OMP:0007879	decreased resistance to an antimicrobial agent	Relative to: OMP_AN:27550 Genotype differences: +lacZ4787(del)( +ptsI745(del) -lacZ4787(del) Condition differences: no differences	temperature:37C medium:LB agar medium:Ethidium Bromide	ECO:0007005	high throughput direct assay evidence used in manual assertion	PMID:21185072	towards some (CHEBI:4883) Ethidium Bromide	chemical concentration= 50 ug/ml normalized fitness= -3.12
OMP_AN:31669		OMP:0007075	decreased growth factor requirement	Relative to: OMP_AN:27606 Genotype differences: +lacZ4787(del)( +ptsI745(del) -lacZ4787(del) Condition differences: no differences	temperature:37C medium:M9 medium:N-Acetyl-D-glucosamine	ECO:0007005	high throughput direct assay evidence used in manual assertion	PMID:21185072	towards some (CHEBI:506227) N-Acetyl-D-glucosamine	chemical concentration= 0.15% normalized fitness= -10.43
OMP_AN:31670		OMP:0007070	decreased organic carbon source utilization	Relative to: OMP_AN:27616 Genotype differences: +lacZ4787(del)( +ptsI745(del) -lacZ4787(del) Condition differences: no differences	temperature:37C medium:M9 medium:N-Acetyl-D-glucosamine	ECO:0007005	high throughput direct assay evidence used in manual assertion	PMID:21185072	towards some (CHEBI:506227) N-Acetyl-D-glucosamine	chemical concentration= 0.15% normalized fitness= -10.43
OMP_AN:31671		OMP:0007075	decreased growth factor requirement	Relative to: OMP_AN:27602 Genotype differences: +lacZ4787(del)( +ptsI745(del) -lacZ4787(del) Condition differences: no differences	temperature:37C medium:M9 medium:D-(+) Glucosamine hydrochloride	ECO:0007005	high throughput direct assay evidence used in manual assertion	PMID:21185072	towards some (CID:91431) D-(+) Glucosamine hydrochloride	chemical concentration= 0.2% normalized fitness= -9.7
OMP_AN:31672		OMP:0007070	decreased organic carbon source utilization	Relative to: OMP_AN:27612 Genotype differences: +lacZ4787(del)( +ptsI745(del) -lacZ4787(del) Condition differences: no differences	temperature:37C medium:M9 medium:D-(+) Glucosamine hydrochloride	ECO:0007005	high throughput direct assay evidence used in manual assertion	PMID:21185072	towards some (CID:91431) D-(+) Glucosamine hydrochloride	chemical concentration= 0.2% normalized fitness= -9.7
OMP_AN:31673		OMP:0007075	decreased growth factor requirement	Relative to: OMP_AN:27603 Genotype differences: +lacZ4787(del)( +ptsI745(del) -lacZ4787(del) Condition differences: no differences	temperature:37C medium:M9 medium:D-Glucose anhydrous	ECO:0007005	high throughput direct assay evidence used in manual assertion	PMID:21185072	towards some (CHEBI:42758) D-Glucose anhydrous	chemical concentration= 0.2% normalized fitness= -9.51
OMP_AN:31674		OMP:0007070	decreased organic carbon source utilization	Relative to: OMP_AN:27613 Genotype differences: +lacZ4787(del)( +ptsI745(del) -lacZ4787(del) Condition differences: no differences	temperature:37C medium:M9 medium:D-Glucose anhydrous	ECO:0007005	high throughput direct assay evidence used in manual assertion	PMID:21185072	towards some (CHEBI:42758) D-Glucose anhydrous	chemical concentration= 0.2% normalized fitness= -9.51
OMP_AN:31675		OMP:0007075	decreased growth factor requirement	Relative to: OMP_AN:27604 Genotype differences: +lacZ4787(del)( +ptsI745(del) -lacZ4787(del) Condition differences: no differences	temperature:37C medium:M9 medium:Glycerol	ECO:0007005	high throughput direct assay evidence used in manual assertion	PMID:21185072	towards some (CHEBI:17754) Glycerol	chemical concentration= 0.4% normalized fitness= -3.17
OMP_AN:31676		OMP:0007070	decreased organic carbon source utilization	Relative to: OMP_AN:27614 Genotype differences: +lacZ4787(del)( +ptsI745(del) -lacZ4787(del) Condition differences: no differences	temperature:37C medium:M9 medium:Glycerol	ECO:0007005	high throughput direct assay evidence used in manual assertion	PMID:21185072	towards some (CHEBI:17754) Glycerol	chemical concentration= 0.4% normalized fitness= -3.17
OMP_AN:31677		OMP:0007075	decreased growth factor requirement	Relative to: OMP_AN:27605 Genotype differences: +lacZ4787(del)( +ptsI745(del) -lacZ4787(del) Condition differences: no differences	temperature:37C medium:M9 medium:D-(+) Maltose monohydrate	ECO:0007005	high throughput direct assay evidence used in manual assertion	PMID:21185072	towards some (CID:23615261) D-(+) Maltose monohydrate	chemical concentration= 0.1% normalized fitness= -13.35
OMP_AN:31678		OMP:0007070	decreased organic carbon source utilization	Relative to: OMP_AN:27615 Genotype differences: +lacZ4787(del)( +ptsI745(del) -lacZ4787(del) Condition differences: no differences	temperature:37C medium:M9 medium:D-(+) Maltose monohydrate	ECO:0007005	high throughput direct assay evidence used in manual assertion	PMID:21185072	towards some (CID:23615261) D-(+) Maltose monohydrate	chemical concentration= 0.1% normalized fitness= -13.35
OMP_AN:31679		OMP:0006063	increased resistance to tetracycline	Relative to: OMP_AN:27523 Genotype differences: +lacZ4787(del)( +ptsI745(del) -lacZ4787(del) Condition differences: no differences	temperature:37C medium:LB agar medium:Minocycline hydrochloride	ECO:0007005	high throughput direct assay evidence used in manual assertion	PMID:21185072	towards some (CHEBI:50697) Minocycline hydrochloride	chemical concentration= 0.2 ug/ml normalized fitness= 3.55
OMP_AN:31680		OMP:0007075	decreased growth factor requirement	Relative to: OMP_AN:27600 Genotype differences: +lacZ4787(del)( +ptsI745(del) -lacZ4787(del) Condition differences: no differences	temperature:37C medium:MOPS medium:NH4Cl	ECO:0007005	high throughput direct assay evidence used in manual assertion	PMID:21185072	towards some (CHEBI:31206) NH4Cl	chemical concentration= 9.5mm normalized fitness= -3.03
OMP_AN:31681		OMP:0007032	decreased utilization of inorganic nitrogen source	Relative to: OMP_AN:27610 Genotype differences: +lacZ4787(del)( +ptsI745(del) -lacZ4787(del) Condition differences: no differences	temperature:37C medium:MOPS medium:NH4Cl	ECO:0007005	high throughput direct assay evidence used in manual assertion	PMID:21185072	towards some (CHEBI:31206) NH4Cl	chemical concentration= 9.5mm normalized fitness= -3.03
OMP_AN:31682		OMP:0007879	decreased resistance to an antimicrobial agent	Relative to: OMP_AN:27503 Genotype differences: +lacZ4787(del)( +ptsI745(del) -lacZ4787(del) Condition differences: no differences	temperature:37C medium:LB agar medium:Nitrofurantoin	ECO:0007005	high throughput direct assay evidence used in manual assertion	PMID:21185072	towards some (CHEBI:71415) Nitrofurantoin	chemical concentration= 1.5 ug/ml normalized fitness= -4.47
OMP_AN:31683		OMP:0006057	decreased resistance to aminoglycoside	Relative to: OMP_AN:27527 Genotype differences: +lacZ4787(del)( +ptsI745(del) -lacZ4787(del) Condition differences: no differences	temperature:37C medium:LB agar medium:Spectinomycin dihydrochloride pentahydrate	ECO:0007005	high throughput direct assay evidence used in manual assertion	PMID:21185072	towards some (CHEBI:9217) Spectinomycin dihydrochloride pentahydrate	chemical concentration= 4 ug/ml normalized fitness= -3.77
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References

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